Tissue-engineered cholecyst-derived extracellular matrix: a biomaterial for in vivo autologous bladder muscular wall regeneration

Kajbafzadeh, Abdol‐Mohammad; Sabetkish, Shabnam; Heidari, Reza; Ebadi, Maryam

doi:10.1007/s00383-014-3474-1

Cited by 20 publications

(12 citation statements)

References 23 publications

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“…Decellularization and further in vivo application of some scaffolds have been discussed in our previous articles (Kajbafzadeh et al . , , ,b,c; Sabetkish et al . ,b).…”

Section: Discussionmentioning

confidence: 99%

A novel technique for simultaneous whole‐body and multi‐organ decellularization: umbilical artery catheterization as a perfusion‐based method in a sheep foetus model

Kajbafzadeh

Khorramirouz

Akbarzadeh

et al. 2015

Int J Experimental Path

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The aim of this study was to develop a method to generate multi-organ acellular matrices. Using a foetal sheep model have developed a method of systemic pulsatile perfusion via the umbilical artery which allows for simultaneous multi-organ decellularization. Twenty sheep foetuses were systemically perfused with Triton X-100 and sodium dodecyl sulphate. Following completion of the whole-body decellularization, multiple biopsy samples were taken from different parts of 21 organs to ascertain complete cell component removal in the preserved extracellular matrices. Both the natural and decellularized organs were subjected to several examinations. The samples were obtained from the skin, eye, ear, nose, throat, cardiovascular, respiratory, gastrointestinal, urinary, musculoskeletal, central nervous and peripheral nervous systems. The histological results depicted well-preserved extracellular matrix (ECM) integrity and intact vascular structures, without any evidence of residual cellular materials, in all decellularized bioscaffolds. Scanning electron microscope (SEM) and biochemical properties remained intact, similar to their age-matched native counterparts. Preservation of the collagen structure was evaluated by a hydroxyproline assay. Dense organs such as bone and muscle were also completely decellularized, with a preserved ECM structure. Thus, as shown in this study, several organs and different tissues were decellularized using a perfusion-based method, which has not been previously accomplished. Given the technical challenges that exist for the efficient generation of biological scaffolds, the current results may pave the way for obtaining a variety of decellularized scaffolds from a single donor. In this study, there have been unique responses to the single acellularization protocol in foetuses, which may reflect the homogeneity of tissues and organs in the developing foetal body.

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“…Decellularization and further in vivo application of some scaffolds have been discussed in our previous articles (Kajbafzadeh et al . , , ,b,c; Sabetkish et al . ,b).…”

Section: Discussionmentioning

confidence: 99%

A novel technique for simultaneous whole‐body and multi‐organ decellularization: umbilical artery catheterization as a perfusion‐based method in a sheep foetus model

Kajbafzadeh

Khorramirouz

Akbarzadeh

et al. 2015

Int J Experimental Path

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“…Cholecyst-derived scaffold (CDS) is a promising scaffold of animal origin that can be used as xenograft for buttressing staples for reinforcing suture lines during abdominal surgery, 16,17 bladder reconstruction, 18 and skin-wound healing. 19 It has been reported that compared with scaffolds isolated from small intestine and urinary bladder, CDS prepared by a non-detergent/enzymatic method contains relatively less residual cellular components.…”

Section: Introductionmentioning

confidence: 99%

Comparative local immunogenic potential of scaffolds prepared from porcine cholecyst, jejunum, and urinary bladder in rat subcutaneous model

et al. 2014

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Extracellular matrices isolated from several mammalian organs/tissues have found several clinical uses as xenografts or implants. However, they may cause complications because of adverse immunologic reactions. Scaffolds that promote favorable graft-acceptance reaction are preferred for fabricating xenografts. The objective of this study was to evaluate the immunogenic potential of a porcine cholecyst-derived scaffold (CDS), prepared by a non-detergent/enzymatic method, in comparison with jejunum and urinary bladder-derived scaffolds in a rat subcutaneous model. Key graft-rejection/acceptance reaction was evaluated at the site of implantation by studying the occurrence and/or function of immunocompetent cells in the tissue reaction. There was differential occurrence of M1-macrophage, M2-macrophage, T-helper cells, T-cytotoxic cells, B-cells, and mast cells in the tissue reaction and the CDS attracted few cells compared with other scaffolds. Real-time polymerase chain reaction for evaluating mRNA of functional markers like inducible nitric oxide synthase (M1 macrophage), arginase 1 (M2 macrophage), interferon gamma (TH1 lymphocytes), and interlukin-4 (TH2 lymphocytes) suggested that the CDS, compared with the scaffolds prepared from small intestine and urinary bladder, elicited M2 macrophage and TH2 lymphocyte polarization that are congenial graft-acceptance reactions. The results indicated that CDS has less immunogenic potential compared with the scaffolds prepared from jejunum and urinary bladder when used as subcutaneous graft in rats. It was concluded that CDS is a promising animal-derived xenograft for biomedical application.

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“…No evidence of inflammatory response was revealed 12 weeks postoperatively, and after 24 weeks seeded grafts resulted in whole bladder and muscular layer regeneration and in new micro vessel formation, suggesting that fragment-seeded cholecyst-derived ECM can enhance the properties of acellular gallbladder in terms of bladder wall regeneration [147]. Even if a decellularized scaffold seems to be the direct and straightforward solution for tissue engineering of tissues and organs, several issues need to be addressed as well.…”

Section: Bladdermentioning

confidence: 99%

Tissue Engineered Scaffolds for an Effective Healing and Regeneration: Reviewing Orthotopic Studies

Baiguera

Urbani

Gaudio

2014

BioMed Research International

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It is commonly stated that tissue engineering is the most promising approach to treat or replace failing tissues/organs. For this aim, a specific strategy should be planned including proper selection of biomaterials, fabrication techniques, cell lines, and signaling cues. A great effort has been pursued to develop suitable scaffolds for the restoration of a variety of tissues and a huge number of protocols ranging from in vitro to in vivo studies, the latter further differentiating into several procedures depending on the type of implantation (i.e., subcutaneous or orthotopic) and the model adopted (i.e., animal or human), have been developed. All together, the published reports demonstrate that the proposed tissue engineering approaches spread toward multiple directions. The critical review of this scenario might suggest, at the same time, that a limited number of studies gave a real improvement to the field, especially referring to in vivo investigations. In this regard, the present paper aims to review the results of in vivo tissue engineering experimentations, focusing on the role of the scaffold and its specificity with respect to the tissue to be regenerated, in order to verify whether an extracellular matrix-like device, as usually stated, could promote an expected positive outcome.

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Tissue-engineered cholecyst-derived extracellular matrix: a biomaterial for in vivo autologous bladder muscular wall regeneration

Abstract: The current study confirmed that autologous fragment-seeded CDECM can be considered as a reliable natural collagen scaffold for bladder augmentation.

Cited by 20 publications

References 23 publications

A novel technique for simultaneous whole‐body and multi‐organ decellularization: umbilical artery catheterization as a perfusion‐based method in a sheep foetus model

A novel technique for simultaneous whole‐body and multi‐organ decellularization: umbilical artery catheterization as a perfusion‐based method in a sheep foetus model

Comparative local immunogenic potential of scaffolds prepared from porcine cholecyst, jejunum, and urinary bladder in rat subcutaneous model

Tissue Engineered Scaffolds for an Effective Healing and Regeneration: Reviewing Orthotopic Studies

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