Tissue engineered in-vitro vascular patch fabrication using hybrid 3D printing and electrospinning

Mayoral, Isabel; Bevilacqua, Elisa; Gómez, Gorka; Hmadcha, Abdelkrim; González-Loscertales, Ignacio; Reina, Esther; Sotelo, Julio; Domínguez, Antonia; Pérez-Alcántara, Pedro; Smani, Younes; González-Puertas, Patricia; Méndez, A.; Uribe, Sergio; Smani, Tarik; Ordóñez, Antonio; Valverde, Israel

doi:10.1016/j.mtbio.2022.100252

Cited by 21 publications

(17 citation statements)

References 81 publications

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“…3D -Printed Materials/Nanofibers Composites for Vascular Graft Applications. With respect to vascular graft materials, Mayoral et al 134 designed a patient-specific patch using a hybrid 3D print in conjunction with vascular smooth muscle cell (VSMC) differentiation. They assessed the most hemodynamically effective aortic patch surgical repair using computational modeling and medical images of a 2-month-old girl with aortic arch hypoplasia.…”

Section: D-printed Materials/nanofibersmentioning

confidence: 99%

Integration of 3D Printing–Coelectrospinning: Concept Shifting in Biomedical Applications

et al. 2023

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Porous structures with sizes between the submicrometer and nanometer scales can be produced using efficient and adaptable electrospinning technology. However, to approximate desirable structures, the construction lacks mechanical sophistication and conformance and requires three-dimensional solitary or multifunctional structures. The diversity of high-performance polymers and blends has enabled the creation of several porous structural conformations for applications in advanced materials science, particularly in biomedicine. Two promising technologies can be combined, such as electrospinning with 3D printing or additive manufacturing, thereby providing a straightforward yet flexible technique for digitally controlled shape-morphing fabrication. The hierarchical integration of configurations is used to imprint complex shapes and patterns onto mesostructured, stimulus-responsive electrospun fabrics. This technique controls the internal stresses caused by the swelling/contraction mismatch in the in-plane and interlayer regions, which, in turn, controls the morphological characteristics of the electrospun membranes. Major innovations in 3D printing, along with additive manufacturing, have led to the production of materials and scaffold systems for tactile and wearable sensors, filtration structures, sensors for structural health monitoring, tissue engineering, biomedical scaffolds, and optical patterning. This review discusses the synergy between 3D printing and electrospinning as a constituent of specific microfabrication methods for quick structural prototypes that are expected to advance into next-generation constructs. Furthermore, individual techniques, their process parameters, and how the fabricated novel structures are applied holistically in the biomedical field have never been discussed in the literature. In summary, this review offers novel insights into the use of electrospinning and 3D printing as well as their integration for cutting-edge applications in the biomedical field.

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Section: D-printed Materials/nanofibersmentioning

confidence: 99%

Integration of 3D Printing–Coelectrospinning: Concept Shifting in Biomedical Applications

et al. 2023

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“…By adjusting the flow rate of microfluidic phase, the size and height of emulsion can be adjusted to produce microcarriers with controllable macropores. 24 Although alginates are used in cell-based therapies are promising for application in tissue engineering and regenerative medicine, there are several major challenges limit its use in cell-based therapies. Alginate microspheres, placed in microenvironment with monovalent cations, are easy to decomposed because of ion exchange reactions of monovalent cations and divalent metal ions, which direct alginate microspheres are difficult to keep mechanical stability in the long term.…”

Section: Alginatementioning

confidence: 99%

“…Wang et al 23 developed a cell microcarriers with controllable macropores and heterogeneous microstructures based on a capillary array microfluidic technology for the formation of AMS with diameter ranging from 45 nm to 1680 μm (Figure 3D). By adjusting the flow rate of microfluidic phase, the size and height of emulsion can be adjusted to produce microcarriers with controllable macropores 24 …”

Section: Natural Polymer‐based Cell Encapsulation Headingmentioning

confidence: 99%

Advances in polymer‐based cell encapsulation and its applications in tissue repair

Xia

Chen

2023

Biotechnology Progress

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Cell microencapsulation is a more widely accepted area of biological encapsulation.In most cases, it involves fixing cells in polymer scaffolds or semi-permeable hydrogel capsules, providing the environment for protecting cells, allowing the exchange of nutrients and oxygen, and protecting cells against the attack of the host immune system by preventing the entry of antibodies and cytotoxic immune cells. Hydrogel encapsulation provides a three-dimensional (3D) environment similar to that experienced in vivo, so it can maintain normal cellular functions to produce tissues similar to those in vivo. Embedded cells can be genetically modified to release specific therapeutic products directly at the target site, thereby eliminating the side effects of systemic treatments. Cellular microcarriers need to meet many extremely high standards regarding their biocompatibility, cytocompatibility, immunoseparation capacity, transport, mechanical, and chemical properties. In this article, we discuss the biopolymer gels used in tissue engineering applications and the brief introduction of cell encapsulation for therapeutic protein production. Also, we review polymer biomaterials and methods for preparing cell microcarriers for biomedical applications. At the same time, in order to improve the application performance of cell microcarriers in vivo, we also summarize the main limitations and improvement strategies of cell encapsulation. Finally, the main applications of polymer cell microcarriers in regenerative medicine are summarized.

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“…The “vascularized tissue engineering chamber” provides vascular support, nutrients, and room for tissue growth by inserting blood vessels into a tissue engineering chamber made of biologically inert materials with a certain degree of hardness ( Hofer et al, 2003 ). This solves the problem that traditional tissue engineering products are difficult to develop and produce stable tissue constructs because nutrition can only be infiltrated by surrounding tissues (200–300 μm) ( Mian et al, 2000 ; Dolderer et al, 2007 ; Eto et al, 2012 ; Kato et al, 2014 ; Dew et al, 2015 ; Mashiko and Yoshimura, 2015 ; Mayoral et al, 2022 ). We consider the construction of an in vivo vascularized tissue engineering chamber of fascia-ear prosthesis model for ear reconstructive surgery by introducing vascular bundles and pre-positioning a 3D-printed ear framework.…”

Section: Introductionmentioning

confidence: 99%

Construction of a vascularized fascia-prosthesis compound model with axial pedicle for ear reconstruction surgery

Chen

Shan

et al. 2023

Front. Bioeng. Biotechnol.

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Background: To design a vascular pedicled fascia-prosthesis compound model that can be used for ear reconstruction surgery.Methods: A vascularized tissue engineering chamber model was constructed in New Zealand rabbits, and fresh tissues were obtained after 4 weeks. The histomorphology and vascularization of the newly born tissue compound were analyzed and evaluated by tissue staining and Micro-CT scanning.Results: The neoplastic fibrous tissue formed in the vascularized tissue engineering chamber with the introduction of abdominal superficial vessels, similar to normal fascia, was superior to the control group in terms of vascularization, vascular density, total vascular volume, and total vascular volume/total tissue volume.Conclusion:In vivo, introducing abdominal superficial vessels in the tissue engineering chamber prepped for ear prosthesis may form a well-vascularized pedicled fascia-prosthesis compound that can be used for ear reconstruction.

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Tissue engineered in-vitro vascular patch fabrication using hybrid 3D printing and electrospinning

Cited by 21 publications

References 81 publications

Integration of 3D Printing–Coelectrospinning: Concept Shifting in Biomedical Applications

Integration of 3D Printing–Coelectrospinning: Concept Shifting in Biomedical Applications

Advances in polymer‐based cell encapsulation and its applications in tissue repair

Construction of a vascularized fascia-prosthesis compound model with axial pedicle for ear reconstruction surgery

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