Tissue-engineered PLLA/gelatine nanofibrous scaffold promoting the phenotypic expression of epithelial and smooth muscle cells for urethral reconstruction

Liu, Guochang; Fu, Ming; Li, Feng; Fu, Wen; Zhang, Zhao; Xia, Hongmei; Niu, Yuqing

doi:10.1016/j.msec.2020.110810

Cited by 53 publications

(48 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Scaffold for bone defect repair should possessed good mechanical properties so that to provide enough support for cell and tissue [ 41 ]. In the present study, compressive properties of the composite scaffolds were examined.…”

Section: Resultsmentioning

confidence: 99%

Accelerated degradation of HAP/PLLA bone scaffold by PGA blending facilitates bioactivity and osteoconductivity

Shuai

Yang

Feng

et al. 2021

Bioactive Materials

277

124

View full text Add to dashboard Cite

The incorporation of hydroxyapatite (HAP) into poly- l -lactic acid (PLLA) matrix serving as bone scaffold is expected to exhibit bioactivity and osteoconductivity to those of the living bone. While too low degradation rate of HAP/PLLA scaffold hinders the activity because the embedded HAP in the PLLA matrix is difficult to contact and exchange ions with body fluid. In this study, biodegradable polymer poly (glycolic acid) (PGA) was blended into the HAP/PLLA scaffold fabricated by laser 3D printing to accelerate the degradation. The results indicated that the incorporation of PGA enhanced the degradation rate of scaffold as indicated by the weight loss increasing from 3.3% to 25.0% after immersion for 28 days, owing to the degradation of high hydrophilic PGA and the subsequent accelerated hydrolysis of PLLA chains. Moreover, a lot of pores produced by the degradation of the scaffold promoted the exposure of HAP from the matrix, which not only activated the deposition of bone like apatite on scaffold but also accelerated apatite growth. Cytocompatibility tests exhibited a good osteoblast adhesion, spreading and proliferation, suggesting the scaffold provided a suitable environment for cell cultivation. Furthermore, the scaffold displayed excellent bone defect repair capacity with the formation of abundant new bone tissue and blood vessel tissue, and both ends of defect region were bridged after 8 weeks of implantation.

show abstract

Section: Resultsmentioning

confidence: 99%

Accelerated degradation of HAP/PLLA bone scaffold by PGA blending facilitates bioactivity and osteoconductivity

Shuai

Yang

Feng

et al. 2021

Bioactive Materials

277

124

View full text Add to dashboard Cite

show abstract

“…However, this polymer does not possess surface receptor sites for cell adhesion; therefore, it is often combined with other natural biopolymers [ 24 ]. Gelatin is a natural hydrophilic polymer and possesses natural cell adhesion sites because of its RGD sequences [ 25 ]. Thus a combination of these biopolymers will provide excellent mechanical and biological properties that are essential for full depth wound healing [ 25 , 26 ].…”

Section: Introductionmentioning

confidence: 99%

Synthesis and Characterization of Exopolysaccharide Encapsulated PCL/Gelatin Skin Substitute for Full-Thickness Wound Regeneration

et al. 2021

View full text Add to dashboard Cite

Loss of skin integrity can lead to serious problems and even death. In this study, for the first time, the effect of exopolysaccharide (EPS) produced by cold-adapted yeast R. mucilaginosa sp. GUMS16 on a full-thickness wound in rats was evaluated. The GUMS16 strain’s EPS was precipitated by adding cold ethanol and then lyophilized. Afterward, the EPS with polycaprolactone (PCL) and gelatin was fabricated into nanofibers with two single-needle and double-needle procedures. The rats’ full-thickness wounds were treated with nanofibers and Hematoxylin and eosin (H&E) and Masson’s Trichrome staining was done for studying the wound healing in rats. Obtained results from SEM, DLS, FTIR, and TGA showed that EPS has a carbohydrate chemical structure with an average diameter of 40 nm. Cell viability assessments showed that the 2% EPS loaded sample exhibits the highest cell activity. Moreover, in vivo implantation of nanofiber webs on the full-thickness wound on rat models displayed a faster healing rate when EPS was loaded into a nanofiber. These results suggest that the produced EPS can be used for skin tissue engineering applications.

show abstract

“…Nanomedicine provides nanomaterials that can be used as a substrate for cell growth and proliferation and can deliver medicine and biological molecules, which eventually results in new tissue or organ replacing the damaged body part under controlled conditions [104]. Among the various nanomedicine techniques is the guided regeneration technique, where synthetic biodegradable nanoplatforms like poly(L-lactic acid) (PLLA), polyglycolic acid (PGA), poly(D,L-lactide-co-glycolide) (PLGA), polycaprolactone (PCL), polyphosphazenes, etc., are used for directing cell growth [105][106][107][108][109].…”

Section: Regenerative Medicinementioning

confidence: 99%

“…Many of the nanomaterials are promising, with appropriate physicochemical properties, and have been subsequently used for neural tissue regeneration applications. These have shown promising outcomes that can help cell attachment and expansion, advance neuronal cell separation and upgrade the recovery of neurons [109]. Some inorganic materials have been used in the regeneration of damaged nerves, such as metallic NPs [108], silica NPs [109], magnetic NPs [110] and quantum dots [111].…”

Section: Regenerative Medicinementioning

confidence: 99%

“…These have shown promising outcomes that can help cell attachment and expansion, advance neuronal cell separation and upgrade the recovery of neurons [109]. Some inorganic materials have been used in the regeneration of damaged nerves, such as metallic NPs [108], silica NPs [109], magnetic NPs [110] and quantum dots [111]. On the other hand, distinctive organic nanomaterials have been studied for neural tissue regeneration applications [112].…”

Section: Regenerative Medicinementioning

confidence: 99%

See 1 more Smart Citation

Nanomaterials for Biomedical Applications: Production, Characterisations, Recent Trends and Difficulties

et al. 2021

View full text Add to dashboard Cite

Designing of nanomaterials has now become a top-priority research goal with a view to developing specific applications in the biomedical fields. In fact, the recent trends in the literature show that there is a lack of in-depth reviews that specifically highlight the current knowledge based on the design and production of nanomaterials. Considerations of size, shape, surface charge and microstructures are important factors in this regard as they affect the performance of nanoparticles (NPs). These parameters are also found to be dependent on their synthesis methods. The characterisation techniques that have been used for the investigation of these nanomaterials are relatively different in their concepts, sample preparation methods and obtained results. Consequently, this review article aims to carry out an in-depth discussion on the recent trends on nanomaterials for biomedical engineering, with a particular emphasis on the choices of the nanomaterials, preparation methods/instruments and characterisations techniques used for designing of nanomaterials. Key applications of these nanomaterials, such as tissue regeneration, medication delivery and wound healing, are also discussed briefly. Covering this knowledge gap will result in a better understanding of the role of nanomaterial design and subsequent larger-scale applications in terms of both its potential and difficulties.

show abstract

Tissue-engineered PLLA/gelatine nanofibrous scaffold promoting the phenotypic expression of epithelial and smooth muscle cells for urethral reconstruction

Cited by 53 publications

References 33 publications

Accelerated degradation of HAP/PLLA bone scaffold by PGA blending facilitates bioactivity and osteoconductivity

Accelerated degradation of HAP/PLLA bone scaffold by PGA blending facilitates bioactivity and osteoconductivity

Synthesis and Characterization of Exopolysaccharide Encapsulated PCL/Gelatin Skin Substitute for Full-Thickness Wound Regeneration

Nanomaterials for Biomedical Applications: Production, Characterisations, Recent Trends and Difficulties

Contact Info

Product

Resources

About