Tissue Engineering

Kaihara, Satoshi; Vacanti, Joseph P.

doi:10.1001/archsurg.134.11.1184

Cited by 44 publications

(4 citation statements)

References 53 publications

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“…Studies using hepatocytes as cellular transplantation revealed the short life span of these injected cells. The introduction of scaffolds was proposed to improve cell viability and function [4].…”

Section: Introductionmentioning

confidence: 99%

Decellularized liver bioscaffold: a histological and immunohistochemical comparison between normal, fibrotic and hepatocellular carcinoma

Salim¹,

Issa

Farrag

et al. 2019

ceh

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Aim of the studyIncreasing demand for liver transplantation represents an important health burden. Decellularized liver bioscaffold can be a suitable alternative for whole organ transplantation. However, various pathologies can affect the structure of decellularized scaffolds.This work discusses differences between hepatic fibrosis (HF), hepatocellular carcinoma (HCC) and normal decellularized liver bioscaffolds.Material and methodsMurine models of HF and HCC were created, livers from normal, HF and HCC were decellularized, and evaluation of decellularization was done using morphological, histological and DNA analysis examination. Also, immunohistochemical staining using collagen, laminin, fibronectin and alphafetoprotein was done. Deposition area and intensity of the used immunohistochemical staining in liver capsules and the staining deposition thickness in the blood vessels and hepatic capsule walls were measured for comparison between the three models.ResultsNormal, HF and HCC livers were decellularized efficiently as confirmed by histological and DNA estimation. HCC decellularized samples showed significantly higher collagen, fibronectin and laminin deposition in both capsule and blood vessels, followed by HF decellularized samples, which also showed the highest thickness of laminin deposition in both capsule and blood vessels, then the normal model, which recorded the lowest value. Alphafetoprotein positive cells were absent in normal and HF, with rare cells in HCC.ConclusionsEven pathologic livers, HF and HCC, can be efficiently decellularized, showing normal morphology and architecture. However, HCC and HF showed significantly higher deposition of extracellular matrix proteins: collagen, fibronectin and laminin. The impact of these differences on physiological and immunological functions of the bioscaffold requires recellularization experiments.

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Section: Introductionmentioning

confidence: 99%

Decellularized liver bioscaffold: a histological and immunohistochemical comparison between normal, fibrotic and hepatocellular carcinoma

Salim¹,

Issa

Farrag

et al. 2019

ceh

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“…Hip and knee implants harbour problems, principally infection, tissue rejection, limited lifespan of the implant and unsatisfactory cosmesis. Furthermore, the costs of technically challenging surgery and prolonged high‐input hospital care have led to research for alternative therapies [5].…”

Section: Introductionmentioning

confidence: 99%

Biomaterials and scaffold design: key to tissue‐engineering cartilage

Raghunath

Rollo

Sales

et al. 2007

Biotech and App Biochem

190

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Cartilage remains one of the most challenging tissues to reconstruct or replace, owing to its complex geometry in facial structures and mechanical strength at articular surfaces in joints. This non-vascular tissue has poor replicative capacity and damage results in its functionally inferior repair tissue, fibrocartilage. This has led to a drive for advancements in tissue engineering. The variety of polymers and fabrication techniques available continues to expand. Pore size, porosity, biocompatibility, shape specificity, integration with native tissue, degradation tailored to rate of neocartilage formation and cost efficiency are important factors which need consideration in the development of a scaffold. The present review considers the current polymers and fabrication methodologies used in scaffold engineering for cartilage and postulates whether we are closer to developing the ideal scaffold for clinical application.

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“…Kaihara et al [1] defined cartilage tissue engineering as "A new field, tissue engineering, applies the principles of biology and engineering toward the development of biological substitutes that restore, maintain, or improve tissue function". There is no blood vessel in cartilage tissue, and the cell composition is single, so cartilage tissue engineering is progressing rapidly.…”

mentioning

confidence: 99%

Research and progress of cartilage tissue-engineering scaffold materials

Zhai¹,

Wu²

2015

DCC

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Due to the limited self healing capacity of human cartilage, the repair of defects gives rise to a challenging clinical problem. Cartilage tissue engineering provides a new method to solve cartilage repair. However, the search for a suitable biological vector material has long been the focus of research interest in this regard. In this paper, the present situation of cartilage tissue engineering vector materials is reviewed.

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Tissue Engineering

Cited by 44 publications

References 53 publications

Decellularized liver bioscaffold: a histological and immunohistochemical comparison between normal, fibrotic and hepatocellular carcinoma

Decellularized liver bioscaffold: a histological and immunohistochemical comparison between normal, fibrotic and hepatocellular carcinoma

Biomaterials and scaffold design: key to tissue‐engineering cartilage

Research and progress of cartilage tissue-engineering scaffold materials

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