Tissue Engineering

Burg, Karen J.L.; Thomas, Chuck B.

doi:10.1002/9780471740360.ebs1203

Cited by 3 publications

(7 citation statements)

References 23 publications

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“…We further postulate that, through the use of biofabrication, these mobile units may be distributed in a heterogeneous manner throughout a gel to form a biologically relevant engineered tissue (Burg & Boland 2003). We and others have demonstrated the use of mobile units, in the form of beads and granules, to form homogenous cellular composites for a variety of applications (Burg et al 2000;Eiselt et al 2000;Halberstadt et al 2000;Marijnissen et al 2000;McGlohorn et al 2004;Brown et al 2005;Burg 2006); the challenge remains in using select configurations to form heterogeneous, complex tissue structures.…”

Section: (B) Tissue-engineered Test Systemsmentioning

confidence: 97%

“…Our long-term objective is to develop composite engineered-tissue systems, capitalizing on technologies developed for our injectable composite systems for regenerative medicine (Burg et al 2000;McGlohorn et al 2004;Brown et al 2005;Burg 2006;Gomillion et al 2007). We have focused on building a toolbox of techniques and methodologies to allow the development of tissue systems that are specific to a particular goal-i.e.…”

Section: Establishment Of the Relevant Toolbox And Challenges In Creamentioning

confidence: 99%

“…Consequently, we first suggested that the positive attributes of a gel could be enhanced by incorporating smaller, more mobile units of rigid scaffold materials (Burg et al 2000;Burg 2006) to accommodate a diversity of cells and structures to form a tissue-engineered composite. We further postulate that, through the use of biofabrication, these mobile units may be distributed in a heterogeneous manner throughout a gel to form a biologically relevant engineered tissue (Burg & Boland 2003).…”

Section: Introductionmentioning

confidence: 99%

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Building off-the-shelf tissue-engineered composites

Burg

Cass

Groff

et al. 2010

Phil. Trans. R. Soc. A.

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Rapid advances in technology have created the realistic possibility of personalized medicine. In 2000, Time magazine listed tissue engineering as one of the ‘hottest 10 career choices’. However, in the past decade, only a handful of tissue-engineered products were translated to the clinical market and none were financially viable. The reality of complex business planning and the high-investment, high-technology environment was not apparent, and the promise of tissue engineering was overstated. In the meantime, biologists were steadily applying three-dimensional benchtop tissue-culture systems for cellular research, but the systems were gelatinous and thus limited in their ability to facilitate the development of complex tissues. Now, the bioengineering literature has seen an emergence of literature describing biofabrication of tissues and organs. However, if one looks closely, again, the viable products appear distant. ‘Rapid’ prototyping to reproduce the intricate patterns of whole organs using large volumes of cellular components faces daunting challenges. Homogenous forms are being labelled ‘tissues’, but, in fact, do not represent the heterogeneous structure of the native biological system. In 2003, we disclosed the concept of combining rapid prototyping techniques with tissue engineering technologies to facilitate precision development of heterogeneous complex tissue-test systems, i.e. systems to be used for drug discovery and the study of cellular behaviour, biomedical devices and progression of disease. The focus of this paper is on the challenges we have faced since that time, moving this concept towards reality, using the case of breast tissue as an example.

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Section: (B) Tissue-engineered Test Systemsmentioning

confidence: 97%

Section: Establishment Of the Relevant Toolbox And Challenges In Creamentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Building off-the-shelf tissue-engineered composites

Burg

Cass

Groff

et al. 2010

Phil. Trans. R. Soc. A.

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“…It's use is mainly due to ease of controlling its mechanical properties and biodegradation rate. [ 3 4 5 ] PLGA hollow tubes have been long studied for use in nerve graft and showed potentially encouraging results. [ 3 4 5 ] Neurotrophic factors within the artificial nerve conduit improved the adhesion, survival and movement of the neuronal cells and assisted alignment of the nerve axons during the regeneration comparable to the nerve regeneration in autografts.…”

Section: Introductionmentioning

confidence: 99%

“…[ 3 4 5 ] PLGA hollow tubes have been long studied for use in nerve graft and showed potentially encouraging results. [ 3 4 5 ] Neurotrophic factors within the artificial nerve conduit improved the adhesion, survival and movement of the neuronal cells and assisted alignment of the nerve axons during the regeneration comparable to the nerve regeneration in autografts. [ 6 7 ] Previously, artificial nerve conduits filled with neural-differentiated human mesenchymal stem cells (hMSCs) and Schwann cells have been studied for use in nerve graft.…”

Section: Introductionmentioning

confidence: 99%

Sciatic nerve repair with tissue engineered nerve: Olfactory ensheathing cells seeded poly(lactic-co-glygolic acid) conduit in an animal model

Tan

Htwe

et al. 2013

IJOO

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Background and Aim:Synthetic nerve conduits have been sought for repair of nerve defects as the autologous nerve grafts causes donor site morbidity and possess other drawbacks. Many strategies have been investigated to improve nerve regeneration through synthetic nerve guided conduits. Olfactory ensheathing cells (OECs) that share both Schwann cell and astrocytic characteristics have been shown to promote axonal regeneration after transplantation. The present study was driven by the hypothesis that tissue-engineered poly(lactic-co-glycolic acid) (PLGA) seeded with OECs would improve peripheral nerve regeneration in a long sciatic nerve defect.Materials and Methods:Sciatic nerve gap of 15 mm was created in six adult female Sprague-Dawley rats and implanted with PLGA seeded with OECs. The nerve regeneration was assessed electrophysiologically at 2, 4 and 6 weeks following implantation. Histopathological examination, scanning electron microscopic (SEM) examination and immunohistochemical analysis were performed at the end of the study.Results:Nerve conduction studies revealed a significant improvement of nerve conduction velocities whereby the mean nerve conduction velocity increases from 4.2 ΁ 0.4 m/s at week 2 to 27.3 ΁ 5.7 m/s at week 6 post-implantation (P < 0.0001). Histological analysis revealed presence of spindle-shaped cells. Immunohistochemical analysis further demonstrated the expression of S100 protein in both cell nucleus and the cytoplasm in these cells, hence confirming their Schwann-cell-like property. Under SEM, these cells were found to be actively secreting extracellular matrix.Conclusion:Tissue-engineered PLGA conduit seeded with OECs provided a permissive environment to facilitate nerve regeneration in a small animal model.

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Cellulosic-Based Conductive Hydrogels for Electro-Active Tissues: A Review Summary

Gebeyehu

Sui

Adamu

et al. 2022

Gels

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The use of hydrogel in tissue engineering is not entirely new. In the last six decades, researchers have used hydrogel to develop artificial organs and tissue for the diagnosis of real-life problems and research purposes. Trial and error dominated the first forty years of tissue generation. Nowadays, biomaterials research is constantly progressing in the direction of new materials with expanded capabilities to better meet the current needs. Knowing the biological phenomenon at the interaction among materials and the human body has promoted the development of smart bio-inert and bio-active polymeric materials or devices as a result of vigorous and consistent research. Hydrogels can be tailored to contain properties such as softness, porosity, adequate strength, biodegradability, and a suitable surface for adhesion; they are ideal for use as a scaffold to provide support for cellular attachment and control tissue shapes. Perhaps electrical conductivity in hydrogel polymers promotes the interaction of electrical signals among artificial neurons and simulates the physiological microenvironment of electro-active tissues. This paper presents a review of the current state-of-the-art related to the complete process of conductive hydrogel manufacturing for tissue engineering from cellulosic materials. The essential properties required by hydrogel for electro-active-tissue regeneration are explored after a short overview of hydrogel classification and manufacturing methods. To prepare hydrogel from cellulose, the base material, cellulose, is first synthesized from plant fibers or generated from bacteria, fungi, or animals. The natural chemistry of cellulose and its derivatives in the fabrication of hydrogels is briefly discussed. Thereafter, the current scenario and latest developments of cellulose-based conductive hydrogels for tissue engineering are reviewed with an illustration from the literature. Finally, the pro and cons of conductive hydrogels for tissue engineering are indicated.

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Tissue Engineering

Cited by 3 publications

References 23 publications

Building off-the-shelf tissue-engineered composites

Building off-the-shelf tissue-engineered composites

Sciatic nerve repair with tissue engineered nerve: Olfactory ensheathing cells seeded poly(lactic-co-glygolic acid) conduit in an animal model

Cellulosic-Based Conductive Hydrogels for Electro-Active Tissues: A Review Summary

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