Tissue engineering: a new paradigm for periodontal regeneration based on molecular and cell biology

Bartold, P. Mark; McCulloch, C; Narayanan, A. Sampath; Pitaru, S.

doi:10.1034/j.1600-0757.2000.2240113.x

Cited by 393 publications

(373 citation statements)

References 95 publications

(131 reference statements)

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“…Cell migration generally plays roles in tissue formation and wound healing as tissue repair takes place following an injury, which involves the processes of inflammation, new tissue formation, and scar formation. 2,3) The migration of hPDL cells is also a critical function in the initiation of periodontal wound healing. 2,4) Transforming growth factor b1 (TGF-b1) is a multifunctional peptide that regulates various cellular activities, including proliferation, differentiation, and expression of extracellular matrix proteins.…”

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confidence: 99%

“…2,3) The migration of hPDL cells is also a critical function in the initiation of periodontal wound healing. 2,4) Transforming growth factor b1 (TGF-b1) is a multifunctional peptide that regulates various cellular activities, including proliferation, differentiation, and expression of extracellular matrix proteins. 5) TGF-b1 is a potent modulator of tissue repair in various tissues.…”

mentioning

confidence: 99%

“…2,4) Transforming growth factor b1 (TGF-b1) is a multifunctional peptide that regulates various cellular activities, including proliferation, differentiation, and expression of extracellular matrix proteins.…”

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confidence: 99%

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Transforming Growth Factor .BETA.1 Promotes Migration of Human Periodontal Ligament Cells through Heat Shock Protein 27 Phosphorylation

Kwon

Kim

Fujii

et al. 2011

Biological & Pharmaceutical Bulletin

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The human periodontal ligament (hPDL) is a highly specialized connective tissue located between the tooth root and alveolar bone. Once the hPDL is severely damaged by periodontal disease, its regeneration is known to be difficult. 1) hPDL cells can serve as an in vitro model to determine the effects of growth factors implicated in periodontal repair or regeneration. Cell migration generally plays roles in tissue formation and wound healing as tissue repair takes place following an injury, which involves the processes of inflammation, new tissue formation, and scar formation.2,3) The migration of hPDL cells is also a critical function in the initiation of periodontal wound healing. 2,4) Transforming growth factor b1 (TGF-b1) is a multifunctional peptide that regulates various cellular activities, including proliferation, differentiation, and expression of extracellular matrix proteins.5) TGF-b1 is a potent modulator of tissue repair in various tissues. It was reported that TGF-b1 alone or in combination with other growth factors accelerates various phases of wound healing.6,7) TGF-b1 may also play an important role in the modulation of tissue formation and development of the periodontium.8) The addition of TGF-b1 to gingival and periodontal ligament fibroblast cell cultures was shown to enhance RNA and protein synthesis. 9)Heat shock protein 27 (Hsp27) acts as ATP-independent molecular chaperone.10) In addition to its chaperone function, Hsp27 also seems to be an important regulator of structural integrity and membrane stability, actin polymerization and intermediate filament cytoskeleton formation, cell migration, epithelial cell-cell adhesion, cell cycle progression, proinflammatory gene expression, signal transduction pathways, mRNA stabilization, differentiation, and apoptosis.11,12) Previous results strongly suggested that phosphorylated Hsp27 was critically involved in the migration process, possibly through the association with signal transducer and activator of transcription 3 (STAT3) and focal adhesion kinase (FAK).13) Cell migration is regulated by various signaling pathway including the b1 integrin, Akt, extracellular signalregulated kinase (ERK) and nuclear factor-kappaB-dependent pathways through TGF-b1 in human cancer cells.14,15) In our previous study, we confirmed that TGF-b1-induced Hsp27 activation promotes the migration of mouse dental papilla-derived MDPC-23 cells.16) Dentinal healing and repair are the result of successive and interrelated processes that include proliferation, chemotaxis, and differentiation of dental pulp cells into odontoblasts, leading to reparative dentin formation. 17) However, the underlying mechanism of TGF-b1 responsible for regulating hPDL cell migration has not been fully elucidated. Because hPDL cells are responsible for tissue regeneration and mineralized tissue matrix formation, and cell migration are critical processes in tissue regeneration, it is valuable to clarify the precise mechanism of the cell migration of hPDL cells. In this study, we therefore investig...

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Transforming Growth Factor .BETA.1 Promotes Migration of Human Periodontal Ligament Cells through Heat Shock Protein 27 Phosphorylation

Kwon

Kim

Fujii

et al. 2011

Biological & Pharmaceutical Bulletin

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“…It is believed that periodontal regeneration can be successfully attained via the migration of periodontal ligament stem cells (4,2). Seo et al verified that only some of the progenitor cells of PDL can be considered stem cells.…”

Section: Introductionmentioning

confidence: 99%

“…Successful periodontal regeneration includes re-establishment of the epithelial seal, formation of connective tissue fibers attached to the root surface, regeneration of acellular cementum on the root surface, and obtaining adequate alveolar bone height. To achieve this goal, several techniques have been recommended for use in the clinical setting, such as guided tissue regeneration (GTR); implantation of autografts, allografts or alloplastic materials; chemical root conditioning; use of growth factors; or a combination of these approaches (1,2). one of the newest methods for regenerating lost tissue is using stem cells.…”

Section: Introductionmentioning

confidence: 99%

Effect of Calcitriol on Differentiation of Periodontal Ligament Stem Cells to Osteoblasts

Soheilifar¹,

Amiri²,

Bidgoli³

et al. 2016

Avicenna J Dent Res

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Growth factors and cytokines modulate gene expression of cell-surface proteoglycans in human periodontal ligament cells

Worapamorn

Haase

et al. 2001

J. Cell. Physiol.

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Cell-surface proteoglycans have been known to be involved in many functions including interactions with components of the extracellular microenvironment, and act as co-receptors which bind and modify the action of various growth factors and cytokines. The purpose of this study was to determine the regulation by growth factors and cytokines on cell-surface proteoglycan gene expression in cultured human periodontal ligament (PDL) cells. Subconfluent, quiescent PDL cells were treated with various concentrations of serum, bFGF, PDGF-BB, TGF-beta1, IL-beta1, and IFN-gamma. RT-PCR technique was used, complemented with Northern blot for syndecan-1, to examine the effects of these agents on the mRNA expression of five cell-surface proteoglycans (syndecan-1, syndecan-2, syndecan-4, glypican and betaglycan). Syndecan-1 mRNA levels increased in response to serum, bFGF and PDGF-BB, but decreased in response to TGF-beta1, IL-1beta and IFN-gamma. In contrast, syndecan-2 mRNA levels were upregulated by TGF-beta1 and IL-1beta stimulation, but remained unchanged with the other agents. Betaglycan gene expression decreased in response to serum, but was upregulated by TGF-beta1 and unchanged by the other stimulants. Additionally, syndecan-4 and glypican were not significantly altered in response to the regulator molecules studied, with the exception that glypican is decreased in response to IFN-gamma. These data demonstrate that the gene expression of the five cell-surface proteoglycans studied is differentially regulated in PDL cells lending support to the notion of distinct functions for these cell-surface proteoglycans.

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Tissue engineering: a new paradigm for periodontal regeneration based on molecular and cell biology

Cited by 393 publications

References 95 publications

Transforming Growth Factor .BETA.1 Promotes Migration of Human Periodontal Ligament Cells through Heat Shock Protein 27 Phosphorylation

Transforming Growth Factor .BETA.1 Promotes Migration of Human Periodontal Ligament Cells through Heat Shock Protein 27 Phosphorylation

Effect of Calcitriol on Differentiation of Periodontal Ligament Stem Cells to Osteoblasts

Growth factors and cytokines modulate gene expression of cell-surface proteoglycans in human periodontal ligament cells

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