Tissue engineering of a bioartificial kidney

Cieslinski, D A; Humes, H. David

doi:10.1002/bit.260430718

Cited by 89 publications

(41 citation statements)

References 20 publications

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“…Also, it allows for transplantation of cells from nonhuman origin, i.e., xenografts or genetically engineered cells, [1][2][3][4] which overcomes the obstacle of limited supply of donor tissue. Because of these benefits, the feasibility of transplanting cells in immunoisolating membranes is under study for the treatment of a wide variety of endocrine diseases such as diabetes mellitus, 5 central nervous system insufficiencies, 6 anemia, 7 dwarfism, 3 Hemophilia B, 8 pituitary, 9 kidney inadequacies, 10 and liver 11 failure. A commonly used procedure for immunoisolation is microencapsulation of tissues in alginate-poly-Llysine (PLL)-based capsules as originally described by Lim and Sun.…”

Section: Introductionmentioning

confidence: 99%

Tissue responses against immunoisolating alginate‐PLL capsules in the immediate posttransplant period

Vos

Hoogmoed

Haan

et al. 2002

J. Biomed. Mater. Res.

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Alginate-polylysine (PLL) capsules are commonly applied for immunoisolation of living cells for the treatment of a wide variety of diseases. Large-scale application of the technique, however, is hampered by insufficient biocompatibility of the capsules with failure of the grafts as a consequence. Most studies addressing biocompatibility issues of alginate-PLL capsules have focused on the degree of overgrowth on the capsules after graft failure and not on the reaction against the capsules in the immediate posttransplant period. Therefore, capsules were implanted in the peritoneal cavity of rats and retrieved 1, 5, and 7 days later for histological examination and X-ray photoelectron spectroscopy analysis for evaluation of chemical changes at the capsule surface. After implantation, the nitrogen signal increased from 5% on day 0, to 8.6% on day 7, illustrating protein adsorption on the capsule's surface. This increase in protein content of the membrane was accompanied by an increase in the percentage of overgrown capsules from 0.5 +/- 0.3% on day 1 to 3.3 +/- 1.6% on day 7. The cellular overgrowth was composed of monocytes/macrophages, granulocytes, fibroblasts, erythrocytes, multinucleated giant cells, and basophils. This overgrowth was not statical as generally assumed but rather dynamic as illustrated by our observation that at day 1 after implantation we mainly found monocytes/macrophages and granulocytes that on later time points were substituted by fibroblasts. As the inflammatory reaction predictably interfere with survival of encapsulated cells, efforts should be made to suppress activities or recruitment of inflammatory cells. These efforts may be temporary rather than permanent because most inflammatory cells have disappeared after 2 weeks of implantation.

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Section: Introductionmentioning

confidence: 99%

Tissue responses against immunoisolating alginate‐PLL capsules in the immediate posttransplant period

Vos

Hoogmoed

Haan

et al. 2002

J. Biomed. Mater. Res.

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“…Also, it allows for transplantation of cells from nonhuman origin, i.e., xenografts, which overcomes the obstacle of limited supply of donor tissue. Because of these benefits, the feasibility of transplanting cells in immunoprotective membranes is under study for the treatment of a wide variety of endocrine diseases, including anemia, 1 dwarfism, 2 hemophilia B, 3 kidney 4 and liver 5 failure, pituitary 6 and central nervous system insufficiencies, 7 and diabetes mellitus. 8 A commonly used procedure for immunoprotection is microencapsulation of tissues in alginate-poly-Llysine (PLL)-based capsules as originally described by Lim and Sun.…”

Section: Introductionmentioning

confidence: 99%

Chemistry and biocompatibility of alginate‐PLL capsules for immunoprotection of mammalian cells

Vos

Hoogmoed

Busscher

2002

J. Biomed. Mater. Res.

104

106

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Transplantation of encapsulated living cells is a promising approach for the treatment of a wide variety of diseases. Large-scale application of the technique, however, is hampered by insufficient biocompatibility of the capsules. In order to get means to study factors influencing the biocompatibility of capsule for encapsulation of living cells, we have correlated the chemical composition of the surface of commonly applied alginate-PLL capsules with the biological response in rats. Capsules prepared of alginates with an intermediate guluronic (G) acid content proved to be biocompatible, whereas capsules prepared of high-G alginates were overgrown by inflammatory cells. We applied X-ray photoelectron spectroscopy to correlate the biological responses with the chemical compositions of the capsule surfaces. High-G alginate capsules proved to have a higher PLL content but less surface binding sites for PLL than low-G alginates. This study, shows for the first time that biological responses against capsules can be successfully correlated to its chemical characteristics.

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“…In einem anderen Versuchsansatz wurden Röhrchen aus Polysulfon mit extrazellulärer Matrix behaftet und mit renal tubulären und endothelialen Zellen besiedelt [58]. Dieses System zeigte einen permiselektiven konvektiven Flüssigkeits-transfer und einen aktiven Transport von [62].…”

Section: Niereunclassified

Tissue engineering in der Urologie

Bartsch

Atala

2003

Urologe [A]

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Tissue engineering is a rather new field of science. Despite this fact, some experimental investigations have already been applied in clinical studies. Compared to other medical fields, tissue engineering in urology is well established. Tissue-engineered bulking agents and tissue-engineered bladder augments are being investigated in clinical trials. Even though the knowledge gained in recent years is promising, the results of cellular therapies need to be critically judged before being finally applied in patients. Genetic engineering and stem cell research (adult undifferentiated cells) have had major impact on the field of tissue engineering over the past 2 years. By using the technology of genetic engineering, biochemical and functional qualities of tissues may be modified. Adult stem cells may help to substitute lost tissue in an autologous fashion by isolating undifferentiated cells from the body and by differentiating them into a desired cell type. These cells may be used to form native functional tissue to replace a diseased organ or organ part.

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Tissue engineering of a bioartificial kidney

Cited by 89 publications

References 20 publications

Tissue responses against immunoisolating alginate‐PLL capsules in the immediate posttransplant period

Tissue responses against immunoisolating alginate‐PLL capsules in the immediate posttransplant period

Chemistry and biocompatibility of alginate‐PLL capsules for immunoprotection of mammalian cells

Tissue engineering in der Urologie

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