Tissue engineering of biphasic cartilage constructs using various biodegradable scaffolds: an in vitro study

Wang, Xuanhui; Grogan, Shawn P.; Rieser, Franz; Winkelmann, Verena; Maquet, Véronique; Berge, Martine La; Mainil‐Varlet, Pierre

doi:10.1016/j.biomaterials.2003.10.102

Cited by 105 publications

(64 citation statements)

References 29 publications

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“…Polylactic/polyglycolic acids, both individually and in combination have been investigated as scaffold material to repair cartilage defects for more than two decades [3,94,168,171,172].…”

Section: Scaffoldsmentioning

confidence: 99%

“…Compared to fibrin, collagen, Poly(L-lactic acid) (PLLA), and poly (DL-lactic-co-glycolic acid) (PLGA), PGA was shown to provide a better scaffold for in vitro cartilage regeneration, as demonstrated by cell densities equivalent to those found in natural tissues, and by continuous cellular production of type II collagen [174]. Although such engineered constructs have also been tested for articular cartilage repair in animal models, mainly in rabbits [94,171,[175][176][177]), they have not been applied in human patients. The possible reasons include the graft induction of foreign body giant cell reaction [100] and the hydrolytic activity of the polymer substrate, which yields both toxic and partially cytotoxic degradation products.…”

Section: Scaffoldsmentioning

confidence: 99%

“…The second approach aims to produce neocartilaginous tissue combining cells with various biomaterials, bioreactor systems and growth factor cocktails [89][90][91][92][93][94]. Both type of cell-based approaches comprise isolation of cells from a low-load bearing area and subsequent expansion in vitro.…”

mentioning

confidence: 99%

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Cartilage tissue engineering for degenerative joint disease☆

Nešić

Whiteside

Brittberg

et al. 2006

Advanced Drug Delivery Reviews

211

156

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Pain in the joint is often due to cartilage degeneration and represents a serious medical problem affecting people of all ages. Although many, mostly surgical techniques, are currently employed to treat cartilage lesions, none has given satisfactory results in the long term. Recent advances in biology and material science have brought tissue engineering to the forefront of new cartilage repair techniques. The combination of autologous cells, specifically designed scaffolds, bioreactors, mechanical stimulations and growth factors together with the knowledge that underlies the principles of cell biology offers promising avenues for cartilage tissue regeneration. The present review explores basic biology mechanisms for cartilage reconstruction and summarizes the advances in the tissue engineering approaches. Furthermore, the limits of the new methods and their potential application in the osteoarthritic conditions are discussed.

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“…Polylactic/polyglycolic acids, both individually and in combination have been investigated as scaffold material to repair cartilage defects for more than two decades [3,94,168,171,172].…”

Section: Scaffoldsmentioning

confidence: 99%

Section: Scaffoldsmentioning

confidence: 99%

See 1 more Smart Citation

Cartilage tissue engineering for degenerative joint disease☆

Nešić

Whiteside

Brittberg

et al. 2006

Advanced Drug Delivery Reviews

211

156

View full text Add to dashboard Cite

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“…Tissue engineering applications aim to replace or regenerate damaged tissues through the combinations of cells, three-dimensional scaffolds and signalling molecules [4][5]. A number of strategies have been implemented to engineer osteochondral constructs including bi-phasic scaffolding [6][7][8][9][10][11][12], bioreactor technologies [13][14][15][16], and growth factor/gene delivery [17][18][19][20]. Engineered anatomically accurate osteochondral grafts have also been suggested as a potential approach to joint condyle repair [21][22].…”

Section: Introductionmentioning

confidence: 99%

Engineering osteochondral constructs through spatial regulation of endochondral ossification

et al. 2013

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Chondrogenically primed bone marrow derived mesenchymal stem cells (MSCs) have been shown to become hypertrophic and undergo endochondral ossification when implanted in vivo. Modulating this endochondral phenotype may be an attractive approach to engineering the osseous phase of an osteochondral implant. The objective of this study was to engineer an osteochondral tissue by promoting endochondral ossification in one layer of a bi-layered construct and stable cartilage in the other. The top-half of bi-layered agarose hydrogels were seeded with culture expanded chondrocytes (termed chondral layer) and the bottom half of the bi-layered agarose hydrogels with MSCs (termed osseous layer). Constructs were cultured in a chondrogenic medium for 21 days and thereafter were either maintained in a chondrogenic medium, transferred to a hypertrophic medium, or implanted subcutaneously into nude mice. This structured chondrogenic bi-layered co-culture was found to enhance chondrogenesis in the chondral layer, appearing to help re-establish the chondrogenic phenotype that is lost in chondrocytes during monolayer expansion. Furthermore, the bilayered co-culture appeared to suppress hypertrophy and mineralisation in the osseous layer.The addition of hypertrophic factors to the media was found to induce mineralisation of the osseous layer in vitro. A similar result was observed in vivo where endochondral ossification was restricted to the osseous layer of the construct leading to the development of an osteochondral tissue. This novel approach represents a potential new treatment strategy for the repair of osteochondral defects.

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“…Currently, the majority of cartilage tissue engineering scaffolds are seeded with fullthickness chondrocytes derived from all the three zones of articular cartilage. [29][30][31] Given the promising results of the aforementioned cell-based approaches to interface tissue engineering, this study will compare the response of DZC, the cells residing in the deep zone of cartilage and directly above the calcified cartilage interface, 32 with that of the commonly used full-thickness chondrocytes. It is hypothesized that the presence of the HA phase within the alginate hydrogel scaffold will promote the formation of a calcified cartilage matrix, and differences in biosynthesis due to cell population are also expected.…”

Section: Introductionmentioning

confidence: 99%

A Hydrogel-Mineral Composite Scaffold for Osteochondral Interface Tissue Engineering

Khanarian

Jiang

Wan

et al. 2012

Tissue Engineering Part A

109

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Osteoarthritis is the leading cause of physical disability among Americans, and tissue engineered cartilage grafts have emerged as a promising treatment option for this debilitating condition. Currently, the formation of a stable interface between the cartilage graft and subchondral bone remains a significant challenge. This study evaluates the potential of a hybrid scaffold of hydroxyapatite (HA) and alginate hydrogel for the regeneration of the osteochondral interface. Specifically, the effects of HA on the response of chondrocytes were determined, focusing on changes in matrix production and mineralization, as well as scaffold mechanical properties over time. Additionally, the optimal chondrocyte population for interface tissue engineering was evaluated. It was observed that the HA phase of the composite scaffold promoted the formation of a proteoglycan-and type II collagen-rich matrix when seeded with deep zone chondrocytes. More importantly, the elevated biosynthesis translated into significant increases in both compressive and shear moduli relative to the mineral-free control. Presence of HA also promoted chondrocyte hypertrophy and type X collagen deposition. These results demonstrate that the hydrogel-calcium phosphate composite supported the formation of a calcified cartilage-like matrix and is a promising scaffold design for osteochondral interface tissue engineering.

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Tissue engineering of biphasic cartilage constructs using various biodegradable scaffolds: an in vitro study

Cited by 105 publications

References 29 publications

Cartilage tissue engineering for degenerative joint disease☆

Cartilage tissue engineering for degenerative joint disease☆

Engineering osteochondral constructs through spatial regulation of endochondral ossification

A Hydrogel-Mineral Composite Scaffold for Osteochondral Interface Tissue Engineering

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