Tissue Engineering of the Bladder—Reality or Myth? A Systematic Review

Sloff, Marije; Simaioforidis, Vasileios; Vries, Rob de; Oosterwijk, Egbert; Feitz, W.F.J.

doi:10.1016/j.juro.2014.03.116

Cited by 53 publications

(38 citation statements)

References 45 publications

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“…A systematic review of 28 preclinical studies was performed to evaluate the potential of tissue engineering of the bladder [59]. In this review, outcomes were compared with available clinical evidence.…”

Section: Bladder Reconstructionmentioning

confidence: 99%

Genitourinary System

Abolbashari

Atala

Yoo

2015

Translational Regenerative Medicine

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Section: Bladder Reconstructionmentioning

confidence: 99%

Genitourinary System

Abolbashari

Atala

Yoo

2015

Translational Regenerative Medicine

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“…The field has progressed markedly over recent decades, and stands poised to provide viable clinical therapies for the repair of skin 1 , cartilage 2,3 , bladder 4,5 , vasculature 6 and bone 7 . Research is increasingly being focused on the regeneration of 'complex' tissues with multi-layered, multi-cellular and highly vascularised structures 8 .…”

Section: Introduction: the Tissue Engineering Imperativementioning

confidence: 99%

Tissue engineering with gellan gum

et al. 2016

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Engineering complex tissues for research and clinical applications relies on high-performance biomaterials that are amenable to biofabrication, maintain mechanical integrity, support specific cell behaviours, and, ultimately, biodegrade. In most cases, complex tissues will need to be fabricated from not one, but many biomaterials, which collectively fulfill these demanding requirements. Gellan gum is an anionic polysaccharide with potential to fill several key roles in engineered tissues, particularly after modification and blending. This review focuses on the present state of research into gellan gum, from its origins, purification and modification, through processing and biofabrication options, to its performance as a cell scaffold for both soft tissue and load bearing applications. Overall, we find gellan gum to be a highly versatile backbone material for tissue engineering research, upon which a broad array of form and functionality can be built. Disciplines Engineering | Physical Sciences and Mathematics Publication DetailsStevens, L. R., Gilmore, K. J., Wallace, G. Engineering complex tissues for research and clinical applications relies on high-performance biomaterials that are amenable to biofabrication, maintain mechanical integrity, support specific cell behaviours, and, ultimately, biodegrade. In most cases, complex tissues will need to be fabricated from not one, but many biomaterials, which collectively fulfill these demanding requirements. Gellan gum is an anionic polysaccharide with potential to fill several key roles in engineered tissues, particularly after modification and blending. This review focuses on the present state of research into gellan gum, from its origins, purification and modification, through processing and biofabrication options, to its performance as a cell scaffold for both soft tissue and load bearing applications. Overall, we find gellan gum to be a highly versatile backbone material for tissue engineering research, upon which a broad array of form and functionality can be built.

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“…8 Unfortunately, postoperative bladder capacity was not augmented compared to preoperative status in large animals according to a systematic review. 9 Although collagen-based scaffolds showed promising short-term outcomes in a pilot clinical trial, 10 the favorable outcomes in preclinical studies were not substantiated in recent clinical trials that tested the long-term follow-up of polyglycolic-acid (PGA)/poly (lactic-co-glycolic acid) (PLGA) copolymer and small intestinal submucosa (SIS). 11,12 Synthetic polymers such as PGA and PLGA were denoted by its inclination to inammation and brosis, as well as unsatised degradation rate.…”

Section: Introductionmentioning

confidence: 99%

Comparison of morphological and functional restoration between asymmetric bilayer chitosan and bladder acellular matrix graft for bladder augmentation in a rat model

Xiao

Wang

et al. 2017

RSC Adv.

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Because of the limitations of current scaffolds and unfavorable results of clinical trials, proper scaffolds facilitating bladder reconstruction are highly desirable. The aim of this study was to evaluate a novel asymmetric bilayer chitosan scaffold compared with conventional bladder acellular matrix graft (BAMG) in a rat model of bladder augmentation. Twenty-four 8 week-old male Sprague-Dawley rats were randomly assigned to the chitosan scaffold, BAMG and cystotomy groups. The rats' bladders were sampled for cystography and routine histological examination at 21 and 70 days. Immunofluorescence photometry, conscious cystometry, quantitative real-time polymerase chain reaction, and western blot analyses were performed using bladders at 70 days. Compared with BAMG, the chitosan scaffold consisted of a membrane-like compact layer and a sponge-like porous layer with an excellent combination of mechanical strength and flexibility. The chitosan group showed better performances than the BAMG group in radiographic cystography, smooth muscle regeneration, blood vessel numbers and functional restoration. In contrast to reduced bladder compliance induced by BAMG, bladder augmented by chitosan displayed nearly 1.5-fold increased bladder capacity with comparable compliance to that of the cystotomy group at 70 days. The chitosan group exhibited higher levels of VEGF and VEGFR2, associated with the activation of the hypoxia-related SDF-1a/CXCR4 pathway. These results suggested that the asymmetric bilayer chitosan scaffold is a promising scaffold for bladder reconstruction.

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Tissue Engineering of the Bladder—Reality or Myth? A Systematic Review

Cited by 53 publications

References 45 publications

Genitourinary System

Genitourinary System

Tissue engineering with gellan gum

Comparison of morphological and functional restoration between asymmetric bilayer chitosan and bladder acellular matrix graft for bladder augmentation in a rat model

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