Tissue Engineering Scaffolds

Das, Suman; Hollister, S. J.

doi:10.1016/b0-08-043152-6/01880-5

Cited by 4 publications

(1 citation statement)

References 21 publications

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“…Adverse reactions to alloplastic, nonbiological materials result in compromised functional outcome in patients and autogenous grafts can lead to complications elsewhere in the patient [1,2]. Tissue engineering may overcome these limitations by the use of scaffolds that fit into anatomic defects, possess mechanical properties that will bear in vivo loads, enhance tissue in-growth, and produce biocompatible degradation byproducts [3][4][5][6][7][8][9].…”

Section: Introductionmentioning

confidence: 99%

Tissue Engineered Bone Using Polycaprolactone Scaffolds Made by Selective Laser Sintering

et al. 2004

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Polycaprolactone is a bioresorbable polymer that has potential for tissue engineering of bone and cartilage. In this work, we report on the computational design and freeform fabrication of porous polycaprolactone scaffolds using selective laser sintering, a rapid prototyping technique. The microstructure and mechanical properties of the fabricated scaffolds were assessed and compared to designed porous architectures and computationally predicted properties. Compressive modulus and yield strength were within the lower range of reported properties for human trabecular bone. Finite element analysis showed that mechanical properties of scaffold designs and of fabricated scaffolds can be computationally predicted. Scaffolds were seeded with BMP-7 transduced fibroblasts and implanted subcutaneously in immunocompromised mice. Histological evaluation and micro-computed tomography (gCT) analysis confirmed that bone was generated in vivo. Finally, we have demonstrated the clinical application of this technology by producing a prototype mandibular condyle scaffold based on an actual pig condyle.

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Section: Introductionmentioning

confidence: 99%

Tissue Engineered Bone Using Polycaprolactone Scaffolds Made by Selective Laser Sintering

et al. 2004

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Selective Laser Sintering Process Optimization for Layered Manufacturing of CAPA® 6501 Polycaprolactone Bone Tissue Engineering Scaffolds

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Hollister

Das

2005

Journal of Manufacturing Science and Engineering

120

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Tissue engineering combines principles of the life sciences and engineering to replace and repair damaged human tissue. Present tissue engineering methods generally require the use of porous, bioresorbable scaffolds to serve as temporary three-dimensional templates to guide cell attachment, differentiation, proliferation, and subsequent regenerate tissue formation. Such scaffolds are anticipated to play an important role in allowing physicians to simultaneously reconstruct and regenerate damaged human tissues such as bone, cartilage, ligament, and tendon. Recent research strongly suggests that the choice of scaffold material and its internal porous architecture significantly influence regenerate tissue structure and function. However, a lack of versatile biomaterials processing and manufacturing methods capable of meeting the complex geometric and compositional requirements of tissue engineering scaffolds has slowed progress towards fully testing these promising findings. It is widely accepted that layered manufacturing methods such as selective laser sintering (SLS) have the potential to address these requirements. We have investigated SLS as a technique to fabricate tissue engineering scaffolds composed of polycaprolactone (PCL), one of the most widely investigated biocompatible, bioresorbable materials for tissue engineering applications. In this article, we report on our development of optimal SLS processing parameters for CAPA® 6501 PCL powder using systematic factorial design of experiments. Using the optimal parameters, we manufactured test scaffolds with designed porous channels and achieved dimensional accuracy to within 3%–8% of design specifications and densities approximately 94% relative to full density. Finally, using the optimal SLS process parameters, we demonstrated the successful fabrication of bone tissue engineering scaffolds based on actual minipig and human condyle scaffold designs.

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