Tissue engineering strategies for human hair follicle regeneration: How far from a hairy goal?

Castro, Ana Rita; Logarinho, Elsa

doi:10.1002/sctm.19-0301

Cited by 49 publications

(47 citation statements)

References 90 publications

(188 reference statements)

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“…To the present time, stem cell based tissue engineering has emerged as the most thriving approach to achieve de novo HF neogenesis, including stem cell transplantation, stem cell‐derived conditioned medium and stem cell derived exosomes. [ 48,49 ] Recent studies have provided encouraging outcomes to therapeutic potential of stem‐cell based approaches to hair loss management, such as bioengineered follicle germ rearranged from embryonic derived follicle cells [ 50 ] and effective 3D‐printing of skin substitutes with human HFs. [ 51 ] However, despite recent progress in the field, each one of them has its own limitations that need to be overcome, thus clinical applications of tissue engineering strategies for hair loss are still absent.…”

Section: Discussionmentioning

confidence: 99%

Regenerating Hair in Prevascularized Tissue Space Formed by a Controllable Foreign Body Reaction

Yang

Miao

Liu

et al. 2020

Adv Funct Materials

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Intracutaneous transplantation of trichogenic cells is a currently endorsed strategy to realize hair regeneration in vivo. However, skin is not the most advantageous transplant site due to the robust mechanical property and deficient physiological perfusion. Herein, a subcutaneous space made of prevascularized collagen fibers (PVCF) generated by controlling the duration of in situ foreign body reaction is reported. In contrast to skin, an optimally preprogrammed PVCF presents a larger tissue volume (171 mm3), low Young's modulus (1/2‐fold), high flexibility high mechanical energy dissipation rate (1.3‐fold), highly permeable surface structure, and plentiful vascular network. Remarkably, cells transplanted into PVCF suffer less apoptosis and necrosis, and generate more mature hairs (≈289 per site) than that in intracutaneous transplantation (≈177 per site). The RNA‐sequencing profiling indicates similar trends under molecular level. This work provides a promising strategy to improve graft site microenvironment physiologically and mechanically, with a low‐cost and simple fabrication process.

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Section: Discussionmentioning

confidence: 99%

Regenerating Hair in Prevascularized Tissue Space Formed by a Controllable Foreign Body Reaction

Yang

Miao

Liu

et al. 2020

Adv Funct Materials

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“…Although we used accessible cell types as a proof of concept study, cell sources should ideally be of human origin and most preferably autologous to cells in clinical setting. Hence, human DP cells 40 and dermal sheath cup cells 41 from patient’s own hair follicles would be the most promising candidates. Regarding epithelial cell sources, hair follicle stem cells located in the bulge region reportedly possess de novo hair generation ability 42 .…”

Section: Resultsmentioning

confidence: 99%

Hair follicle germs containing vascular endothelial cells for hair regenerative medicine

Kageyama

Chun

Fukuda

2021

Sci Rep

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Hair regenerative medicine has emerged as a promising approach for the treatment of severe hair loss. Recent advances in three-dimensional tissue engineering, such as formation of hair follicle germs (HFGs), have considerably improved hair regeneration after transplantation in animal models. Here, we proposed an approach for fabricating HFGs containing vascular endothelial cells. Epithelial, dermal papilla, and vascular endothelial cells initially formed a single aggregate, which subsequently became a dumbbell-shaped HFG, wherein the vascular endothelial cells localized in the region of dermal papilla cells. The HFGs containing vascular endothelial cells exhibited higher expression of hair morphogenesis-related genes in vitro, along with higher levels of hair shaft regeneration upon transplantation to the dorsal side of nude mice, than those without vascular endothelial cells. The generated hair follicles represented functional characteristics, such as piloerection, as well as morphological characteristics comparable to those of natural hair shafts. This approach may provide a promising strategy for fabricating tissue grafts with higher hair inductivity for hair regenerative medicine.

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“…However, enzymatic method would disrupt specified extracellular matrix microenvironment, which is fundamental for maintaining hair inductive features. Therefore, lots of effort has been made to find out an alternative in vitro expansion procedure to preserve hair inductive ability of DPCs [49].…”

Section: Isolation and Culture Methods Of Dpcsmentioning

confidence: 99%

“…The co-culture of human dermal papilla with keratinocytes is costly and requires the establishment of a cell bank and accurate quality control tests for safety. The co-culture of DPCs with the exogenous Wnt protein is associated with potential problems and the stability of the Wnt gene expression during the cell culture expansion [49, 50].…”

Section: Effective Methods For Maintaining the Potential Hair Inductimentioning

confidence: 99%

“…Biodegradability, biocompatibility, and cost-effectiveness are essential features of scaffolds for hair follicle regeneration, which has been performed using different natural and synthetic scaffolds, including hydrophilic polyvinyl alcohol, chitosan and hydrogel [59][60][61] silk-gelatin, hyaluronic acid, and collagen [62]. In another study, in vitro human hair follicles similar to vellus were recreated with a mixture of DPCs, keratinocytes, and melanocytes in a collagen scaffold, and named "microfollicles" [49]. In a recent study, combination of hydrogel scaffold with mouse adult DPCs and epidermal cells enhances de novo hair follicle formation in mice model.…”

Section: Tissue Engineering Strategies For Human Hair Follicle Regenementioning

confidence: 99%

See 1 more Smart Citation

Maintaining Hair Inductivity in Human Dermal Papilla Cells: A Review of Effective Methods

Taghiabadi

Nilforoushzadeh

Aghdami

2020

Skin Pharmacol Physiol

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The dermal papilla comprises mesenchymal cells in hair follicles, which play the main role in regulating hair growth. Maintaining the potential hair inductivity of dermal papilla cells (DPCs) and dermal sheath cells during cell culture is the main factor in in vitro morphogenesis and regeneration of hair follicles. Using common methods for the cultivation of human dermal papilla reduces the maintenance requirements of the inductive capacity of the dermal papilla and the expression of specific dermal papilla biomarkers. Optimizing culture conditions is therefore crucial for DPCs. Moreover, exosomes appear to play a key role in regulating the hair follicle growth through a paracrine mechanism and provide a functional method for treating hair loss. The present review investigated the biology of DPCs, the molecular and cell signaling mechanisms contributing to hair follicle growth in humans, the properties of the dermal papilla, and the effective techniques in maintaining hair inductivity in DPC cultures in humans as well as hair follicle bioengineering.

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Tissue engineering strategies for human hair follicle regeneration: How far from a hairy goal?

Cited by 49 publications

References 90 publications

Regenerating Hair in Prevascularized Tissue Space Formed by a Controllable Foreign Body Reaction

Regenerating Hair in Prevascularized Tissue Space Formed by a Controllable Foreign Body Reaction

Hair follicle germs containing vascular endothelial cells for hair regenerative medicine

Maintaining Hair Inductivity in Human Dermal Papilla Cells: A Review of Effective Methods

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