Tissue engineering using a combined cell sheet technology and scaffolding approach

Зурина, И.М.; Presniakova, Viktoria S.; Butnaru, Denis; Svistunov, Andrey; Timashev, P. S.; Rochev, Yury

doi:10.1016/j.actbio.2020.06.016

Cited by 50 publications

(37 citation statements)

References 138 publications

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“…As a part of regenerative medicine, the desideratum of tissue engineering (TE) is represented by the fabrication of nonviable complex biocompatible systems that are able to revive the structural integrity and functionality of damaged tissues by restoring, replacing or regenerate them [ 297 ]. Nanostructured biomaterials represent a suitable choice for TE applications, not only because they properly interact with living systems and possess specific and selective therapeutic purpose, but also because they possess versatile and tunable characteristics which enable the achievement of particular requirements, such as (i) biocompatibility (a complex feature that relies on the bidirectional interactions between nanomaterials and host cells or tissues); (ii) physicochemical properties (microstructure, phase transitions, porosity, wettability, morphology, topography, composition, stability, reactivity); and (iii) circumstantial bioactivity [ 298 , 299 ].…”

Section: Silver Nanoparticles For Tissue Engineeringmentioning

confidence: 99%

An Updated Review on Silver Nanoparticles in Biomedicine

et al. 2020

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Silver nanoparticles (AgNPs) represent one of the most explored categories of nanomaterials for new and improved biomaterials and biotechnologies, with impressive use in the pharmaceutical and cosmetic industry, anti-infective therapy and wound care, food and the textile industry. Their extensive and versatile applicability relies on the genuine and easy-tunable properties of nanosilver, including remarkable physicochemical behavior, exceptional antimicrobial efficiency, anti-inflammatory action and antitumor activity. Besides commercially available and clinically safe AgNPs-based products, a substantial number of recent studies assessed the applicability of nanosilver as therapeutic agents in augmented and alternative strategies for cancer therapy, sensing and diagnosis platforms, restorative and regenerative biomaterials. Given the beneficial interactions of AgNPs with living structures and their nontoxic effects on healthy human cells, they represent an accurate candidate for various biomedical products. In the present review, the most important and recent applications of AgNPs in biomedical products and biomedicine are considered.

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Section: Silver Nanoparticles For Tissue Engineeringmentioning

confidence: 99%

An Updated Review on Silver Nanoparticles in Biomedicine

et al. 2020

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“…In cell sheet engineering, the cells cultured on the surface of a stimulus‐sensitive polymer are released as an intact cell sheet after the formation of confluence cell sheet without the need for proteolytic enzymes, which avoids damages on the deposited ECM (Takahashi & Okano, 2019). In the cell sheet technology, unlike the conventional method (proteolytic enzymes such as trypsin and dispase, or chelating agents such as EDTA) that damages the cell‐cell junctions and ECM components, the cell–cell and cell‐ECM interactions are intactly preserved and constructed sheets that have a high cellular density with uniform cell distribution, which mimics a natural microenvironment and maintains the mechanical support for the cells (Y. Lu et al, 2019; Zurina et al, 2020). In the cell sheet technology, various factors such as cell surface proteins, cellular communication signals, cytokines, and growth factors are included in the ECM, therefore the cell sheet will have more potential in tissue regeneration.…”

Section: Cell Sheet Engineeringmentioning

confidence: 99%

“…To increase the ECM production, the cultivation time can be extended and a stimulant can be used such as vitamin C. Macromolecular crowding methods can also be applied. Yet, the most practical method is the use of cell sheet combinations with scaffolds supporter (Zurina et al, 2020). Williams et al, 2020).…”

Section: General Overview Of Cell Sheet Strategiesmentioning

confidence: 99%

Corneal endothelium tissue engineering: An evolution of signaling molecules, cells, and scaffolds toward 3D bioprinting and cell sheets

Khalili

Asadi

Kahroba

et al. 2020

Journal Cellular Physiology

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Cornea is an avascular and transparent tissue that focuses light on retina. Cornea is supported by the corneal‐endothelial layer through regulation of hydration homeostasis. Restoring vision in patients afflicted with corneal endothelium dysfunction‐mediated blindness most often requires corneal transplantation (CT), which faces considerable constrictions due to donor limitations. An emerging alternative to CT is corneal endothelium tissue engineering (CETE), which involves utilizing scaffold‐based methods and scaffold‐free strategies. The innovative scaffold‐free method is cell sheet engineering, which typically generates cell layers surrounded by an intact extracellular matrix, exhibiting tunable release from the stimuli‐responsive surface. In some studies, scaffold‐based or scaffold‐free technologies have been reported to achieve promising outcomes. However, yet some issues exist in translating CETE from bench to clinical practice. In this review, we compare different corneal endothelium regeneration methods and elaborate on the application of multiple cell types (stem cells, corneal endothelial cells, and endothelial precursors), signaling molecules (growth factors, cytokines, chemical compounds, and small RNAs), and natural and synthetic scaffolds for CETE. Furthermore, we discuss the importance of three‐dimensional bioprinting strategies and simulation of Descemet's membrane by biomimetic topography. Finally, we dissected the recent advances, applications, and prospects of cell sheet engineering for CETE.

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“…Tissue engineering is an interdisciplinary subject that utilizes the principles of engineering and life science to study and develop bioactive articial substitutes for the maintenance, restoration, or construction of human damaged tissues. 1,2 The fundamental goal of tissue engineering is to promote formation and integration of tissue to functional structures or organs. Cells, scaffolds and growth factors are the three elements of tissue engineering.…”

Section: Introductionmentioning

confidence: 99%