Tissue expression pattern of two aquaporin-encoding genes in different organs of the seagrass Posidonia oceanica

Cozza, Radiana; Pangaro, T.

doi:10.1016/j.aquabot.2009.03.007

Cited by 12 publications

(4 citation statements)

References 14 publications

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“…Only in leaves of seedlings kept in hypersaline water, the expression levels of PIPs did not change significantly, suggesting the inability of the seedlings to deal with this stressful condition. The over-expression of PIP1 and PIP2 isoforms analyzed confirmed at the gene level the results of previous studies that showed an increment of both PIP1 transcripts and peptides in P. oceanica leaf tissue following salt stress (Maestrini et al 2004;Cozza and Pangaro 2009;Serra et al 2011), strongly suggesting that both these aquaporins are involved in osmotic balance maintenance in seagrasses. In addition, after 48 h of exposure, PIP1 and PIP2 expression levels were lower than at 24 h, except for PIP2 in seedlings exposed to pH variation.…”

Section: Discussionsupporting

confidence: 88%

Reference genes assessment for the seagrass Posidonia oceanica in different salinity, pH and light conditions

et al. 2012

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The stability on the expression level of putative reference genes (RGs) for reverse transcriptase-quantitative PCR (RT-qPCR) was tested in the seagrass Posidonia oceanica for plants collected in different environmental conditions (at different depths and in different salinity and pH). Besides adult plants, seedlings were also used for pH and salinity experiments. The putative RGs encompassed the most frequently used in other species to normalize RT-qPCR in a range of experimental conditions. Assignment of the best RGs has been performed with three Excelbased applets, BestKeeper, geNorm and NormFinder, and the results showed that best RGs may change depending on the experimental conditions tested, plant age and on the software utilized. Preferred sets of RGs are proposed for each of the conditions and a general procedure concerning their selection is discussed. In addition, expression levels of four genes involved in plant response to light, salinity and pH variation have also been evaluated.

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Section: Discussionsupporting

confidence: 88%

Reference genes assessment for the seagrass Posidonia oceanica in different salinity, pH and light conditions

et al. 2012

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“…However, in monocots, information on CHL P orthologs exists mainly as a result of sequencing projects (Zea mays, GenBank: NM_001155910, and Oryza sativa, GenBank: Q6Z2T6) or direct database submission (Triticum aestivum, GenBank: DQ139268) (Alexandrov et al 2009, International Rice Genome Sequencing Project 2005. To date, the function of CHL P has not been examined in any monocot, including all of the seagrasses, although a limited number of genes was generally investigated in the latter (Giordani et al 2000, Cozza & Pangaro 2009). In addition, a partial expressed sequence tag (EST) database of P. oceanica reporting 1219 tentative unigenes has recently been released (Wissler et al 2009).…”

Section: Introductionmentioning

confidence: 99%

PoCHL P expression pattern in Posidonia oceanica is related to critical light conditions

Bruno¹,

Bruno²,

Chiappetta³

et al. 2010

Mar. Ecol. Prog. Ser.

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In past decades, meadows of Posidonia oceanica (L) Delile have declined extensively in the Mediterranean basin mainly due to disturbances associated with reduced light availability. To investigate the molecular mechanisms of photoacclimation in this seagrass, we selected the CHL P gene as a marker of plant response to light availability because it is involved in the chlorophylltocopherols biosynthetic pathways. After characterizing the PoCHL P gene, its expression pattern was analyzed in leaves of plants growing in 2 distinct meadows that were previously categorized as disturbed and preserved. Plants were sampled during different seasons and light availability was monitored in the selected meadows over a 1 yr period. PoCHL P expression was also analyzed in leaves of plants that were exposed to controlled light conditions in aquaria. We found that PoCHL P was expressed differentially with respect to leaf developmental stage and that its expression pattern changed rapidly under light conditions far below the compensation value established for P. oceanica. Hence, the recognition of this gene as a reasonable marker of critical light conditions provides an important step towards the functional characterization of stress-related genes in seagrasses. KEY WORDS: PoCHL P expression · Posidonia oceanica · Light availabilityResale or republication not permitted without written consent of the publisher

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“…In Posidonia oceanica (Seagrass), significant role of PIPs have been observed in salinity tolerance and maintaining water balance in the leaves ( Serra et al, 2013 ). Similarly, expression profiling performed by Cozza and Pangaro (2009) also suggested the role of AQPs in salinity and water balance in seagrass. Apart from such few reports, no significant study evaluating physiological role of AQPs has been performed in aquatic plants.…”

Section: Discussionmentioning

confidence: 87%

Understanding Aquaporin Transport System in Eelgrass (Zostera marina L.), an Aquatic Plant Species

et al. 2017

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Aquaporins (AQPs) are a class of integral membrane proteins involved in the transport of water and many other small solutes. The AQPs have been extensively studied in many land species obtaining water and nutrients from the soil, but their distribution and evolution have never been investigated in aquatic plant species, where solute assimilation is mostly through the leaves. In this regard, identification of AQPs in the genome of Zostera marina L. (eelgrass), an aquatic ecological model species could reveal important differences underlying solute uptake between land and aquatic species. In the present study, genome-wide analysis led to the identification of 25 AQPs belonging to four subfamilies, plasma membrane intrinsic proteins (PIPs), tonoplast intrinsic proteins (TIPs), nodulin 26-like intrinsic proteins (NIPs), small basic intrinsic proteins (SIPs) in eelgrass. As in other monocots, the XIP subfamily was found to be absent from the eelgrass genome. Further classification of subfamilies revealed a unique distribution pattern, namely the loss of the NIP2 (NIP-III) subgroup, which is known for silicon (Si) transport activity and ubiquitously present in monocot species. This finding has great importance, since the eelgrass population stability in natural niche is reported to be associated with Si concentrations in water. In addition, analysis of available RNA-seq data showed evidence of expression in 24 out of the 25 AQPs across four different tissues such as root, vegetative tissue, male flower and female flower. In contrast to land plants, higher expression of PIPs was observed in shoot compared to root tissues. This is likely explained by the unique plant architecture of eelgrass where most of the nutrients and water are absorbed by shoot rather than root tissues. Similarly, higher expression of the TIP1 and TIP5 families was observed specifically in male flowers suggesting a role in pollen maturation. This genome-wide analysis of AQP distribution, evolution and expression dynamics can find relevance in understanding the adaptation of aquatic and land species to their respective environments.

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Tissue expression pattern of two aquaporin-encoding genes in different organs of the seagrass Posidonia oceanica

Cited by 12 publications

References 14 publications

Reference genes assessment for the seagrass Posidonia oceanica in different salinity, pH and light conditions

Reference genes assessment for the seagrass Posidonia oceanica in different salinity, pH and light conditions

PoCHL P expression pattern in Posidonia oceanica is related to critical light conditions

Understanding Aquaporin Transport System in Eelgrass (Zostera marina L.), an Aquatic Plant Species

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