Tissue-specific engineering: 3D bioprinting in regenerative medicine

Wang, Zhen; Kapadia, Wasim; Li, Cuidi; Lin, Feng Huei; Pereira, Rúben F.; Granja, Pedro L.; Sarmento, Bruno; Cui, Wenguo

doi:10.1016/j.jconrel.2020.11.044

Cited by 59 publications

(20 citation statements)

References 199 publications

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“…In the past decade, tissue engineering has attracted great attention as an alternative to traditional tissue regeneration methods, − and great attempts have been made within this field to synthesize and manufacture scaffolds, which can improve tissue regeneration. − Hydrogel is a kind of hydrophilic polymer material with a lightly cross-linked three-dimensional network structure, which is known for absorbing and retaining a large amount of water while maintaining its own structure insoluble in water. , For wound healing, hydrogels can provide a moist environment for the wound site, absorb exudates, and clean up the local environment to accelerate healing without causing toxicity. − Polyethylene glycol (PEG) is one of the most important raw materials for the preparation of hydrogels. , It has the characteristics of non-toxicity, low immunogenicity, and good biocompatibility and can be excreted through the kidneys without accumulation in the body. , In tissue engineering, scaffolds that can be degraded and remodeled as cells that migrate and synthesize a new extracellular matrix are considered to be more conducive to long-term tissue regeneration . In our previous work, we have successfully synthesized a variety of PEG hydrogels and these hydrogels have great potential in tissue engineering scaffolds. − In addition, our previous research work has also proven that the degradation performance of PEG hydrogels can be tuned to meet different needs by changing the ratio of the degradable cross-linker and the non-degradable cross-linker. , In this research, we also introduced hydrolysis degradable ester groups to endow the hydrogel with degradable properties, thereby making it as a degradable wound dressing.…”

Section: Introductionmentioning

confidence: 99%

Injectable and Degradable PEG Hydrogel with Antibacterial Performance for Promoting Wound Healing

Liu

Jiang

Guo

et al. 2021

ACS Appl. Bio Mater.

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Injectable and degradable PEG hydrogel was prepared via Michael-type addition between cross-linking monomer 4-arm-PEG-MAL and two cross-linkers of hydrolysis degradable PEG-diester-dithiol and non-degradable PEG-dithiol, and it had a porous structure with the uniform pore size. The biocompatibility assays in vitro indicated that PEG hydrogel had excellent biocompatibility and can be degraded naturally without leading to any negative impact on cells. The results of antibacterial experiments showed that PEG hydrogel can inhibit the growth of bacteria. Furthermore, the Cell Counting Kit-8 (CCK-8) assay, LIVE/DEAD cell staining, and scratch healing experiments proved that PEG hydrogel can promote cell proliferation and migration, which had been further confirmed in in vivo experiments on the rat wound models. All experimental results demonstrated that PEG hydrogel is an injectable antibacterial dressing, which can promote the process of wound healing and has great potential in the field of wound healing.

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Section: Introductionmentioning

confidence: 99%

Injectable and Degradable PEG Hydrogel with Antibacterial Performance for Promoting Wound Healing

Liu

Jiang

Guo

et al. 2021

ACS Appl. Bio Mater.

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“…Despite the great advances in 3D bioprinting techniques, many challenges are still present. Among them are the printing strategies, multifactorial process to choose the appropriate bioink material, design of the scaffolds, cell vitality, mechanical and heterogenic characters of the printed scaffolds [ 449 ]. Moreover, the 4D bioprinting approach presents a time factor that enables the 3D printed scaffolds to present stimuli-responsive modulation of their shape and/or function.…”

Section: Smart/stimuli-responsive Hydrogels For 3d and 4d Bioprintingmentioning

confidence: 99%

Smart/stimuli-responsive hydrogels: Cutting-edge platforms for tissue engineering and other biomedical applications

El-Husseiny

Mady

Hamabe

et al. 2022

Materials Today Bio

223

139

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Recently, biomedicine and tissue regeneration have emerged as great advances that impacted the spectrum of healthcare. This left the door open for further improvement of their applications to revitalize the impaired tissues. Hence, restoring their functions. The implementation of therapeutic protocols that merge biomimetic scaffolds, bioactive molecules, and cells plays a pivotal role in this track. Smart/stimuli-responsive hydrogels are remarkable three-dimensional (3D) bioscaffolds intended for tissue engineering and other biomedical purposes. They can simulate the physicochemical, mechanical, and biological characters of the innate tissues. Also, they provide the aqueous conditions for cell growth, support 3D conformation, provide mechanical stability for the cells, and serve as potent delivery matrices for bioactive molecules. Many natural and artificial polymers were broadly utilized to design these intelligent platforms with novel advanced characteristics and tailored functionalities that fit such applications. In the present review, we highlighted the different types of smart/stimuli-responsive hydrogels with emphasis on their synthesis scheme. Besides, the mechanisms of their responsiveness to different stimuli were elaborated. Their potential for tissue engineering applications was discussed. Furthermore, their exploitation in other biomedical applications as targeted drug delivery, smart biosensors, actuators, 3D and 4D printing, and 3D cell culture were outlined. In addition, we threw light on smart self-healing hydrogels and their applications in biomedicine. Eventually, we presented their future perceptions in biomedical and tissue regeneration applications. Conclusively, current progress in the design of smart/stimuli-responsive hydrogels enhances their prospective to function as intelligent, and sophisticated systems in different biomedical applications.

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“…Many of the bioprinting approaches mentioned could be adjusted and optimised with or without cell utilisation for bone tissue engineering. The survivability of cells in situ following the printing method is part of many issues related to cell printing [173]. New methods used for obtaining 3D cellcharged structures with proper mechanical and biological properties have been applied with collagen-based bioinks [174].…”

Section: Tissue Engineering (Te)mentioning

confidence: 99%

Additive Manufacturing of Biopolymers for Tissue Engineering and Regenerative Medicine: An Overview, Potential Applications, Advancements, and Trends

Veeman

Sai

Sureshkumar³

et al. 2021

International Journal of Polymer Science

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As a technique of producing fabric engineering scaffolds, three-dimensional (3D) printing has tremendous possibilities. 3D printing applications are restricted to a wide range of biomaterials in the field of regenerative medicine and tissue engineering. Due to their biocompatibility, bioactiveness, and biodegradability, biopolymers such as collagen, alginate, silk fibroin, chitosan, alginate, cellulose, and starch are used in a variety of fields, including the food, biomedical, regeneration, agriculture, packaging, and pharmaceutical industries. The benefits of producing 3D-printed scaffolds are many, including the capacity to produce complicated geometries, porosity, and multicell coculture and to take growth factors into account. In particular, the additional production of biopolymers offers new options to produce 3D structures and materials with specialised patterns and properties. In the realm of tissue engineering and regenerative medicine (TERM), important progress has been accomplished; now, several state-of-the-art techniques are used to produce porous scaffolds for organ or tissue regeneration to be suited for tissue technology. Natural biopolymeric materials are often better suited for designing and manufacturing healing equipment than temporary implants and tissue regeneration materials owing to its appropriate properties and biocompatibility. The review focuses on the additive manufacturing of biopolymers with significant changes, advancements, trends, and developments in regenerative medicine and tissue engineering with potential applications.

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Tissue-specific engineering: 3D bioprinting in regenerative medicine

Cited by 59 publications

References 199 publications

Injectable and Degradable PEG Hydrogel with Antibacterial Performance for Promoting Wound Healing

Injectable and Degradable PEG Hydrogel with Antibacterial Performance for Promoting Wound Healing

Smart/stimuli-responsive hydrogels: Cutting-edge platforms for tissue engineering and other biomedical applications

Additive Manufacturing of Biopolymers for Tissue Engineering and Regenerative Medicine: An Overview, Potential Applications, Advancements, and Trends

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