Tissue-specific functional interaction between apolipoproteins A1 and E in cold-induced adipose organ mitochondrial energy metabolism

Gao

et al. 2022

Genes

Cold stress is an important factor affecting cattle health, production performance, and reproductive efficiency. Understanding of the potential mechanism underlying genetic adaptation to local environments, particularly extreme cold environment, is limited. Here, by using FLK and hapFLK methods, we found that the Zinc finger CCCH-type containing 10 (ZC3H10) gene underwent positive selection in the Menggu, Fuzhou, Anxi, and Shigatse humped cattle breeds that are distributed in the cold areas of China. Furthermore, ZC3H10 expression significantly increased in bovine fetal fibroblast (BFF) cells at 28 °C for 4 h. ZC3H10 knockout BFFs were generated using CRISPR/Cas9. Wild and ZC3H10-deleted BFFs were treated at two temperatures and were divided into four groups (WT, wild and cultured at 38 °C; KO, ZC3H10−/− and 38 °C; WT_LT, wild, and 28 °C for 4 h; and KO_LT, ZC3H10−/− and 28 °C for 4 h. A total of 466, 598, 519, and 650 differently expressed genes (two-fold or more than two-fold changes) were identified by determining transcriptomic difference (KO_LT vs. KO, WT_LT vs. WT, KO vs. WT, and KO_LT vs. WT_LT, respectively). Loss of ZC3H10 dysregulated pathways involved in thermogenesis and immunity, and ZC3H10 participated in immunity-related pathways induced by cold stress and regulated genes involved in glucose and lipid metabolism and lipid transport (PLTP and APOA1), thereby facilitating adaptability to cold stress. Our findings provide a foundation for further studies on the function of ZC3H10 in cold stress and development of bovine breeding strategies for combatting the influences of cold climate.

Section: Discussionmentioning

confidence: 99%

Selection Signature and CRISPR/Cas9-Mediated Gene Knockout Analyses Reveal ZC3H10 Involved in Cold Adaptation in Chinese Native Cattle

Gao

et al. 2022

Genes

Current Opinion in Lipidology

“…Moreover, apo A-I and apo E appear to exert differential tissue-specific effects on thermogenesis. HDL-associated apo A-I stimulates thermogenesis in BAT, a process inhibited by HDL-associated apo E3 [63 ▪▪ ]. In contrast, both HDL-associated apo A-I and apo E3 are necessary to stimulate thermogenesis in WAT [63 ▪▪ ].…”

Section: High-density Lipoprotein and Energy Expenditurementioning

confidence: 99%

“…Furthermore, apo A-I in tandem with apo E2 stimulates thermogenesis in WAT, while apo A-I together with apo E4 triggers heat production in BAT [63 ▪▪ ]. Apo E3 can also be necessary to stimulate heat production in WAT, but it exerts the opposite effect on BAT [63 ▪▪ ].…”

Section: High-density Lipoprotein and Energy Expenditurementioning

confidence: 99%

“…HDL-associated apo A-I stimulates thermogenesis in BAT, a process inhibited by HDL-associated apo E3 [63 ▪▪ ]. In contrast, both HDL-associated apo A-I and apo E3 are necessary to stimulate thermogenesis in WAT [63 ▪▪ ]. The relationship between apo E and thermogenesis regulation is however more complex and depends on the apo E isoform.…”

Section: High-density Lipoprotein and Energy Expenditurementioning

confidence: 99%

See 1 more Smart Citation

Sweet swell of burning fat: emerging role of high-density lipoprotein in energy homeostasis

Kontush,

Martin,

Brites

2023

Purpose of review Metabolism of lipids and lipoproteins, including high-density lipoprotein (HDL), plays a central role in energy homeostasis. Mechanisms underlying the relationship between energy homeostasis and HDL however remain poorly studied. Recent findings Available evidence reveals that HDL is implicated in energy homeostasis. Circulating high-density lipoprotein-cholesterol (HDL-C) levels are affected by energy production, raising with increasing resting metabolic rate. Lipolysis of triglycerides as a source of energy decreases plasma levels of remnant cholesterol, increases levels of HDL-C, and can be cardioprotective. Switch to preferential energy production from carbohydrates exerts opposite effects. Summary Low HDL-C may represent a biomarker of inefficient energy production from fats. HDL-C-raising can be beneficial when it reflects enhanced energy production from burning fat.

“…In terms of the thermogenesis mechanism, the classical BAT mainly stimulates β3-adrenergic receptors to increase intracellular lipolysis through norepinephrine (NE) released by the sympathetic nervous system. Fatty acids are used for oxidation and they bind directly to activate UCP1, which uncouples oxidative phosphorylation and mitochondrial ATP synthesis to generate heat 30 - 32 . During this process, NE activates cyclic adenosine monophosphate (cAMP) through β3-AR on the cell membrane of the adipose tissue, and cAMP stimulates cAMP-dependent protein kinase A to phosphorylate downstream proteins, such as cAMP-responsive element-binding protein (CREB) 33 .…”

Section: The Brown and Beige Adipose Tissue Characteristics Of Thermo...mentioning

confidence: 99%

Regulation of Adipose Thermogenesis and its Critical Role in Glucose and Lipid Metabolism

Wang¹,

Qiu²,

Gan³

et al. 2022

Int. J. Biol. Sci.

The function of the adipose tissue is influenced by complex interactions between genetics, epigenetics, and the environment, and its dysfunction can cause a variety of metabolic diseases, such as obesity or type 2 diabetes (T2D). The beige/brown adipose tissue plays a crucial role in regulating glucose and lipid metabolism by increasing energy metabolism to generate heat. The adipose tissue thermogenic program is a complex network that involves many signaling pathways regulated by coding RNAs (cRNAs) that encode transcription factor, and non-coding RNAs (ncRNAs) including microRNAs (miRNAs) and long noncoding RNAs (lncRNAs). This article discusses factors that regulate adipose tissue thermogenesis, including cRNAs and ncRNAs, and the important role of thermogenic adipose tissue in obesity-related metabolic syndrome. Several studies have shown that some cRNAs and ncRNAs can modulate the thermogenic function of adipose tissue in different ways. This article reviews the roles of cRNAs and ncRNAs in regulating thermogenesis in the beige/brown adipose tissue and the important role of the beige/brown adipose tissue in maintaining the balance of glucose and lipid metabolism in the body.