Tissue-specific patterns of synthesis of heat-shock proteins and thermal tolerance of the fathead minnow (<i>Pimephales promelas</i>)

Dyer, Scott D.; Dickson, Kenneth L.; Zimmerman, Earl G.; Sanders, Brenda Μ.

doi:10.1139/z91-282

Cited by 83 publications

(39 citation statements)

References 21 publications

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“…This is in contrast to previous studies showing heat-shock-induced increases in Hsp70 levels in brain tissue of other fishes, including killifish (Koban et al, 1991), fathead minnow (Dyer et al, 1991), buffalo sculpin (Enophrys bison), speckled sanddab (Citharichthys stigmaeus), and English sole (Parophrys vetulus) (Dietz and Somero, 1993). Similar to our previous studies on green sturgeon fingerlings , there was a high degree of variability in brain Hsc/Hsp70 levels of the most feed restricted green and white sturgeon, regardless of temperature.…”

Section: Hsc/hsp70 Protein Levelscontrasting

confidence: 99%

“…Highly induced Hsp70 levels in response to heat shock in the same tissues have also been reported in other fishes (e.g., fathead minnow, Pimephales promelas (Dyer et al, 1991); mummichog, Fundulus heteroclitus (Koban et al, 1991)). The discrepancy in Hsp70 responses to increased temperature between the current study and Wang et al Fig.…”

Section: Hsc/hsp70 Protein Levelssupporting

confidence: 61%

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Effects of feed restriction on the upper temperature tolerance and heat shock response in juvenile green and white sturgeon

Lee

Hung

Fangue

et al. 2016

Comparative Biochemistry and Physiology Part A: Molecular &

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The objective of the current study was to investigate the effects of feed restriction on whole-organism upper thermal tolerance and the heat shock response of green and white sturgeon to determine how changes in food amount might influence physiological performance of each species when faced with temperature stress. Two parallel feed restriction trials were carried out for juvenile green (202 g; 222-day post hatch: dph) and white sturgeon (205 g; 197-dph) to manipulate nutritional status at 12.5%, 25%, 50%, or 100% of optimum feeding rate (100% OFR were 1.6% and 1.8% body weight/day, respectively) for four weeks. Following the trials, the critical thermal maximum (CTMax, 0.3°C/min) of sturgeon (N = 12/treatment/species) was assessed as an indicator of whole-organism upper thermal tolerance. To assess temperature sensitivity, sturgeon (N = 9/treatment/species) were acutely transferred to two temperature treatments (28°C and 18°C as a handling control) for 2 h followed by 2 h of recovery at 18°C before being sacrificed, and gill, brain, and mucus sampled for measurements of 70-kDa heat shock protein levels (Hsc/Hsp70). Feeding rate had species-specific effects on CTMax in green and white sturgeon such that CTMax of green sturgeon decreased as the magnitude of feed restriction increased; whereas, CTMax of white sturgeon did not change with feed restriction. Elevated temperature (28°C) and feed restriction increased Hsc/Hsp70 levels in the gill tissue of green sturgeon, while heat shock increased Hsc/ Hsp70 levels in the mucus of white sturgeon. Our results suggest that green sturgeon may be more susceptible to temperature stress under food-limited conditions.

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Section: Hsc/hsp70 Protein Levelscontrasting

confidence: 99%

Section: Hsc/hsp70 Protein Levelssupporting

confidence: 61%

Effects of feed restriction on the upper temperature tolerance and heat shock response in juvenile green and white sturgeon

Lee

Hung

Fangue

et al. 2016

Comparative Biochemistry and Physiology Part A: Molecular &

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“…The tissue-specific differences in hsp60 expression found in this study are similar to those observed for hsp70 expression in carp, Cyprinus carpio L. 1758 (Wang et al, 2007). Similarly, tissue-specific hsp70 expression was reported in the killifish Fundulus heteroclitus (L. 1766) (Koban et al, 1991), fathead minnow Pimephales promelas Rafinesque 1820 (Dyer et al, 1991), brown trout Salmo trutta L. 1758 (Schmidt et al, 1998), and sea lamprey Petromyzon marinus L. 1758 (Wood et al, 1999). The fact that stress responses differ between tissues supports the hypothesis that the thermal limits of an organism are governed by certain tissues more than others (Dyer et al, 1991).…”

Section: Discussionsupporting

confidence: 86%

“…Similarly, tissue-specific hsp70 expression was reported in the killifish Fundulus heteroclitus (L. 1766) (Koban et al, 1991), fathead minnow Pimephales promelas Rafinesque 1820 (Dyer et al, 1991), brown trout Salmo trutta L. 1758 (Schmidt et al, 1998), and sea lamprey Petromyzon marinus L. 1758 (Wood et al, 1999). The fact that stress responses differ between tissues supports the hypothesis that the thermal limits of an organism are governed by certain tissues more than others (Dyer et al, 1991). In this study, a prominent induction of hsp60 synthesis was clearly apparent in the liver.…”

Section: Discussionmentioning

confidence: 67%

Short Communication Effect of heat stress on heat-shock protein (Hsp60) mRNA expression in rainbow trout Oncorhynchus mykiss

Shi¹,

Liu²,

Zhang³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. The enhanced expression of heat shock proteins (hsps) in organisms can be detected in response to many kinds of stressor. For fish, high temperature is an important stressor, and hsp expression is associated with differences in environmental temperature. In this study, rainbow trout (Oncorhynchus mykiss) that were accustomed to an aquatic temperature of 18°C were exposed to an elevated temperature (25°C), and hsp60 expression in the gill, liver, spleen, heart, and head kidney was quantified using real-time polymerase chain reaction in unstressed and heat-stressed animals. The fish responded to heat stress in a time-and tissue-specific manner. Cardiac hsp60 mRNA levels were largely unchanged, and the greatest induction of hsp60 in heat-stressed animals was recorded in the liver, suggesting that protein damage and the consequent requirement for the Hsp60 protein are probably greater in hepatic tissue. Therefore, fish must be provided with optimal 5281 Hsp60 expression response to heat stress in trout ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (2): 5280-5286 (2015) temperature conditions in order to realize their potential growth and maximize fish farm profits.

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“…Many different factors, like temperature (Dyer et al 1990), metals (Levinson et al 1980), hypoxia (Sciandra et al 1984), and osmolarity (SheikhHamad et al 1994) can induce perturbations in the physico-chemical environment, becoming stressors for animals living in that environment. Fish are constantly exposed to stressful conditions, and as a consequence have evolved different stress response systems, which have been expressed at all levels of biological organization.…”

Section: Introductionmentioning

confidence: 99%

Expression of stress proteins 70 in tilapia (Oreochromis mossambicus) during confinement and crowding stress

Dini¹,

Lanubile²,

Tarantino³

et al. 2006

Italian Journal of Zoology

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The knowledge of fish biology of stress response to unfavourable environmental conditions is of interest for both basic and applied purpose, providing useful information for fish farming and environmental risk assessment. In this study, tilapias (Oreochromis mossambicus), confined in nets inside the tanks for 4 or 5 h, were examined for induction of Hsp 70 in various organs by western blotting and for cortisol levels in the blood. In net-confined fish, cortisol, primary stress indicator, was found to consistently increase with differences between 4 and 5 h of confinement. Hsp 70 expression, evaluated in striated and cardiac muscle, gill, liver and brain showed fluctuations in confined animals. Indeed, increase or decrease of Hsp 70 expression in stressed fish was modified in organ-and stress duration-dependent manner and subjective fluctuations were minimal. Morphological observations (LM and EM) were carried out on liver, brain and gill from control and stressed animals. In particular, the liver underwent major modifications while the brain was only slightly modified and the gills were unmodified during confinement stress. Taken together, our data suggest that Hsp 70 expression could be an indicator of stress state in fish when measured in the significant responsive organs.

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Tissue-specific patterns of synthesis of heat-shock proteins and thermal tolerance of the fathead minnow (Pimephales promelas)

Cited by 83 publications

References 21 publications

Effects of feed restriction on the upper temperature tolerance and heat shock response in juvenile green and white sturgeon

Effects of feed restriction on the upper temperature tolerance and heat shock response in juvenile green and white sturgeon

Short Communication Effect of heat stress on heat-shock protein (Hsp60) mRNA expression in rainbow trout Oncorhynchus mykiss

Expression of stress proteins 70 in tilapia (Oreochromis mossambicus) during confinement and crowding stress

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