Tissue-specific transcriptional modulation of fatty acid-binding protein genes, fabp2, fabp3 and fabp6, by fatty acids and the peroxisome proliferator, clofibrate, in zebrafish (Danio rerio)

Fatty acid binding proteins have the potential to channel dietary fatty acids into enterocyte nuclei

“…Levels of fabp1b.1 and fabp2 transcripts were upregulated in the anterior intestine after feeding, both at the larval stages and in adults. As previously reported ( 45,47 ), these levels were modulated according to the lipid composition of the diet. Lipid-mediated regulation of gene transcription via the activation of nuclear receptors to modify the expression of genes coding for proteins involved in the lipid metabolism triggered the proliferation of intestinal cells and modulated the expression of molecular signals that may affect homeostasis ( 26,36 ).…”

“…Different fabp genes were expressed in the zebrafi sh intestine ( 41,(44)(45)(46)(47), including high transcript levels of fabp1b.1 and fabp2 and trace amounts of fabp1a and fabp1b.2 ( 44,45,47,65 ). By carefully removing the ventral white adipose tissue closely associated with the digestive tract and dissecting the anterior intestine, which is the major site of fat absorption ( 41-43 ), we retrieved similarly high transcript levels of fabp1b.1 and fabp2 and smaller amounts of fabp1b.2 , irrespective of the animals' nutritional status.…”

“…FABP1 and FABP2 are the most strongly expressed FABP family members in the human small intestine ( 13,14 ), and these proteins are found in abundance in absorptive cells ( 39,40 ). In zebrafi sh, the anterior intestine is the major site of fat absorption (41)(42)(43), and different fabp genes were found expressed in the intestine including fabp1a , fabp1b.1 , fabp1b.2 , fabp2 , fabp3 , fabp6 , fabp7a / fabp7b , fabp10a / fabp10b , and fabp11a ( 41,(44)(45)(46)(47). The teleost ancestor experienced a whole-genome duplication event at the base of the teleost radiation ( 48 ) leading, in some cases, to the retention of pairs of duplicate genes (e.g., fabp1a/fabp1b ).…”

Chronic effects of clofibric acid in zebrafish (Danio rerio): A multigenerational study

“…CA was dosed through the diet, at nominal contents of 1 and 10 mg/g of food and the exposure period was from 5 to 140 dpf. These contents were selected based on human therapeutic doses and according to previous studies that used a similar range of bezofibrate and clofibrate contents incorporated in the diet in zebrafish (Velasco-Santamaria et al, 2011;Venkatachalam et al, 2012;Venkatachalam et al, 2013). CA was incorporated in the diet using acetone as vehicle.…”

“…Considering their effects in mammalian lipid metabolism, these pharmaceuticals might also interfere with lipid homeostasis and growth of non-target exposed animals, like fish as recently shown by the regulation of the genes coding for fatty acid-binding proteins (Venkatachalam et al, 2012;Venkatachalam et al, 2013) and the enzyme fatty acyl-coenzyme-A oxidase (FAO) involved in fatty acid oxidation (Weston et al, 2009). Previous studies on the effects of CA on the growth of fish were inconclusive as both reduced growth (Owen et al, 2010;Raldua et al, 2008) or no effects at all have been observed following exposure (Owen et al, 2010).…”