Tissue Spectroscopy and Optical Clearing of Colorectal Mucosa in the Pursuit of New Cancer Diagnostic Approaches

Fernandes, Luís Gustavo Romani; Silva, Hugo F.; Martins, Inês; Carvalho, Sónia; Carneiro, Isa; Henrique, Rui; Tuchin, Valery V.; Oliveira, Luís

doi:10.18287/jbpe21.07.040302

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

2021

2024

Publication Types

Select...

Article2

Other1

Relationship

Self Cite0

Independent3

Authors

Journals

Cited by 3 publications

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

Optimized reconstruction of the absorption spectra of kidney tissues from the spectra of tissue components using the least squares method

Pinheiro,

Fernandes,

Carneiro

et al. 2024

Journal of Biophotonics

View full text Add to dashboard Cite

With the objective of developing new methods to acquire diagnostic information, the reconstruction of the broadband absorption coefficient spectra (μa[λ]) of healthy and chromophobe renal cell carcinoma kidney tissues was performed. By performing a weighted sum of the absorption spectra of proteins, DNA, oxygenated, and deoxygenated hemoglobin, lipids, water, melanin, and lipofuscin, it was possible to obtain a good match of the experimental μa(λ) of both kidney conditions. The weights used in those reconstructions were estimated using the least squares method, and assuming a total water content of 77% in both kidney tissues, it was possible to calculate the concentrations of the other tissue components. It has been shown that with the development of cancer, the concentrations of proteins, DNA, oxygenated hemoglobin, lipids, and lipofuscin increase, and the concentration of melanin decreases. Future studies based on minimally invasive spectral measurements will allow cancer diagnosis using the proposed approach.

show abstract