Tissue Trace Elements and Lipid Peroxidation in Breeding Female Bank Voles Myodes glareolus

Abstract

Recent studies have demonstrated that reproduction reduces oxidative damage in various tissues of small mammal females. The present work was designed to determine whether the reduction of oxidative stress in reproductive bank vole females was associated with changes in tissue trace elements (iron, copper, zinc) that play an essential role in the production of reactive oxygen species. Lipid peroxidation (a marker of oxidative stress) and iron concentration in liver, kidneys, and skeletal muscles of reproducing bank vole females that weaned one litter were significantly lower than in non-reproducing females; linear regression analysis confirmed a positive relation between the tissue iron and lipid peroxidation. The concentrations of copper were significantly lower only in skeletal muscles of reproductive females and correlated positively with lipid peroxidation. No changes in tissue zinc were found in breeding females when compared with non-breeding animals. These data indicate that decreases in tissue iron and copper concentrations may be responsible for the reduction of oxidative stress in reproductive bank vole females.

Using Machine Learning to Expedite the Screening of Environmental Factors Associated with the Risk of Spontaneous Preterm Birth: From Exposure Mixtures to Key Molecular Events

“…For FA(21:0), as one of the important saturated fatty acids (SFA), we speculate that it may affect the incidence of PB by involving the inflammatory process and fetal development like SFA. − In addition, we found that various metals in maternal serum had a strong correlation with lipid features. Such relationships had been demonstrated by previous studies, e.g., rare earth elements, essential trace elements, and toxic metals . This suggests that high maternal exposure to various metals may be associated with SPB risk due to their interfering with GP metabolism.…”

Total reflection X-ray fluorescence spectrometry for trace determination of iron and some additional elements in biological samples

“…(Abbreviations: - , data not given; RT , room temperature; GFAAS , graphite furnace atomic absorption spectrometry; ICP-OES , inductively coupled plasma-optical emission spectrometry; ICP-MS , inductively coupled plasma-mass spectrometry; HC , hepatocytes; KC , Kupffer cells; LSEC , liver sinusoidal endothelial cells; HSC , hepatic stellate cells; BMDMs , bone marrow–derived macrophages) Measurement technique Sample type Sample preparation (a) Reagents and treatment (b) Duration (c) Approx. sample amount Analyzed elements Validation by recovery in reference material or by reference method LOD Ref Colorimetric assay Rat Brain cells (astrocytes) (a) Lysis in NaOH for 2 h, then mixing with HCl and iron-releasing reagent (mixture of HCl and KMnO 4 ) for 2 h at 60 °C followed by staining with iron detection reagent (ferrozone, neocuproine, ammonium acetate, ascorbic acid) for 30 min (b) 4.5 h (c) 100 μL of cell lysates Fe Validated by GFAAS as reference method - [ 5 ] Mouse Liver (a) Digestion at 90 °C in HNO 3 and H 2 SO 4 followed by addition of H 2 O 2 (b) Duration not given: Incubation in ferrozine, ascorbic acid, Tris, and HCl at pH = 4 30 min (c) Not given Fe - - [ 4 ] GFAAS Mouse Liver Kidney Gastrocnemius muscle (a) Microwave-assisted digestion in a mixture of HNO 3 and H 2 O 2 ; evaporated to dryness and re-dissolved in water at 200 °C (b) 40 min (c) 250 mg Fe Cu Zn SRM 1577c, bovine liver 95–101% 89–95% 90–95% - [ 9 ] Mouse and human Liver Macrophages (a) Digestion in HNO 3 followed by ultrasonication at 30–40 kHz; if necessary appropriate dilution in water (b) > 30 min (c) Not given Fe - - [ 7 ] ICP-OES SRM Bovine liver (a) M...…”

“…Furthermore, the performance of the proposed method was compared with that of other methods reported recently in literature (see Table 4). Most common practices for iron determination in the field of biomedical research have been and still are colorimetric assays, i.e., photometric detection after adequate staining of the samples (e.g., [4,5]), and/or application of GFAAS (e.g., [5,7,9]). Sensitivity of colorimetric assays is typically much lower than that of atomic spectrometric techniques, allowing detection of Fe in a concentration range between a few micrograms per liter to low milligrams per liter.…”

“…A widely used and established approach for iron determination in this field involves staining of the samples and photometric detection, i.e., colorimetric assays using most commonly ferrozine as complexing and staining agent [ 4 – 6 ]. Besides, instrumental atomic spectrometric techniques for quantification, such as graphite furnace atomic absorption (GFAAS), have been used [ 7 – 9 ]. This technique can also be applied to suspensions, slurries, and even solids; however, it is restricted to the determination of single elements and requires external calibration.…”