Tissue transglutaminase antibodies in celiac disease, comparison of an enzyme linked immunosorbent assay and a dot blot assay

Mankaï, Amani; Sakly, Wahiba; Landolsi, Hanène; Gueddah, L.; Sriha, Badreddine; Ayadi, A.; Sfar, Mohamed Tahar; Skandrani, K.; Harbi, A.; Essoussi, A. S.; Korbi, S.; Fabien, Nicole; Jeddi, M.; Ghedira, Ibtissem

doi:10.1016/j.patbio.2004.07.022

Cited by 19 publications

(13 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Anti-endomysium and Anti-tissue Transglutaminase Antibodies Anti-endomysium antibodies were detected by indirect immunofluorescence using human umbilical cord cryostat sections (4 μm) as described previously [21]. Investigation of IgA class antibodies was done using fluorescein labeled anti-human IgA antibodies (Bio-Rad, Marnes-La Coquette, France).…”

Section: Methodsmentioning

confidence: 99%

Anti-Saccharomyces cerevisiae Antibodies are Frequent in Type 1 Diabetes

et al. 2010

View full text Add to dashboard Cite

Anti-Saccharomyces cerevisiae antibodies (ASCA) have been described in many autoimmune diseases in which there is an increased intestinal permeability. Also in type 1 diabetes (T1D), there is an increased intestinal permeability. Since no data are available about ASCA in T1D, we evaluated, retrospectively, the frequency of ASCA in this disease. ASCA, IgG, and IgA, were determined by ELISA in sera of 224 T1D patients in which coeliac disease has been excluded and 157 healthy control group. The frequency of ASCA (IgG or IgA) was significantly higher in T1D patients than in the control group (24.5% vs. 2.5%, p < 10(-7)). The same observation was found in children and in adult patients when we compare them to healthy children and blood donors group respectively. Compared to children, adult patients with T1D showed significantly higher frequencies of ASCA of any isotype (38% vs. 13.7%, p < 10(-4)), both ASCA IgG and IgA (12% vs. 1.6%, p = 0.002), ASCA IgG (35% vs. 9.8%, p < 10(-5)) and ASCA IgA (15% vs. 5.6%, p = 0.001). The frequency of ASCA was statistically higher in females of all T1D than in males (30.8% vs.17.7%, p = 0.03), in girls than in boys (22% vs.6.2%, p = 0.017), and significantly higher in men than in boys (35.7% vs. 6.2%, p < 10(-4)). The frequency of ASCA IgG was significantly higher than that of ASCA IgA in all T1D patients (21% vs. 9.8%, p < 0.002), in all females (26.5% vs. 10.2%, p < 0.002), in women (37.9% vs. 12%, p < 0.001). The frequency of ASCA was significantly higher in all long-term T1D than in an inaugural T1D (29% vs. 14.5%, p = 0.019). The same observation was found in adults (45.8% vs. 17.8%, p = 0.01). In long-term T1D patients, ASCA were significantly more frequent in adults than children (45.8% vs. 14.5%, p < 10(-4)). The frequency of ASCA IgG was significantly higher in long-term T1D than in an inaugural T1D (25.2% vs. 11.6%, p = 0.03). Patients with T1D had a high frequency of ASCA.

show abstract

Section: Methodsmentioning

confidence: 99%

Anti-Saccharomyces cerevisiae Antibodies are Frequent in Type 1 Diabetes

et al. 2010

View full text Add to dashboard Cite

show abstract

“…However, discordant data between adults and children have been reported as the anti-tTG assay is less accurate in very young patients and autoantibody levels can fluctuate (9)(10)(11). In fact, the reported sensitivity for IgA anti-tTG, including all ages, ranges between 67% and 100%, with a specificity between 96% and 100% (5,(11)(12)(13)(14)(15)(16)(17)(18)(19), while in very young patients the sensitivity is lower, with a value ranging from 67% to 83% (11,17,18).…”

Section: Introductionmentioning

confidence: 99%

“…EMA sensitivity in children ranges from 83% to 100% (12,17,18,20), but is lower in children under 2 years of age, being approximately 85% (17,18,20).…”

Section: Introductionmentioning

confidence: 99%

The old and new tests for celiac disease: which is the best test combination to diagnose celiac disease in pediatric patients?

Brusca

Carroccio²,

Tonutti³

et al. 2012

Clinical Chemistry and Laboratory Medicine

View full text Add to dashboard Cite

Background: In the diagnosis of celiac disease (CD), serum assays for anti-endomysium (EMA) and anti-transglutaminase (anti-tTG) antibodies have excellent diagnostic accuracy. However, these assays are less sensitive in young pediatric patients. Recently, a new ELISA test using deamidated gliadin peptides (DGP) as antigen has proved to be very sensitive and specific even in pediatric patients. In addition, anti-actin IgA antibodies (AAA) is another test that can be used in CD patients because antibody concentrations correlate with the degree of villous atrophy. This study evaluated the clinical accuracy of anti-tTG, EMA, AGA, anti-DGP and AAA and the effectiveness of these in different combinations for diagnosing CD in a large cohort of pediatric patients. Methods: Sera of 150 children under 6 years of age were tested: 95 patients had a diagnosis of CD, while 55 patients who did not suffer from CD were used as controls. Anti-DGP IgA/IgG and AAA were assayed with ELISA kits, while anti-tTG IgA/IgG and AGA IgG/IgA were assayed using a quantitative fluoroimmunoassay. The EMA test was conducted by indirect immunofluorescence.

show abstract

“…A new generation of AGA antibody assays has been developed recently to detect antibodies to synthetic deamidated homologous gliadin peptides in the serum of patients with CD (10,11); nevertheless, these tests, although highly sensitive and specific, are not yet widely implemented, and the test results in young children are still lacking. Over the years, in clinical practice the AGA test has given way to the EMA test, because the latter has a sensitivity and specificity approaching 100% (6,7); however data in the literature show that the assay may be less accurate in children younger than 2 years, with a sensitivity of 83% to 87% (5,12,13). As far as anti-TG2 autoantibodies are concerned, they are present in the serum of untreated patients with CD and are now detected by an ELISA test based on the use of human recombinant tissue transglutaminase; the sensitivity of the test in children ranges from 91% to 97%, with a specificity varying from 96% to 100% (10,14,15), these values being similar to those in EMA.…”

mentioning

confidence: 99%