Titania Nanotube Arrays Surface Anodization and Various Human Cell Line Models Adherence Profiles

Abstract

Titania nanotube arrays (TNA) have high biocompatibility, less toxicity, and a large surface area per volume; thus, TNA offer great potential in biomedical applications. Interactions between micro environment and cell on the TNA surface are intensively investigated regarding cell attachment and interaction. Anodization was used to create a highly ordered nano-porous oxide layer with nano-sized pores on the surface of the titanium. This process was carried out at 30 V with a sweep rate of 1 V/sec for a different duration (10 s, 1 min, 2 min, 5 min, 10 min, 20 min, 30 min, 1 h, 2 h, 3 h and 6 h). On an anodized titanium surface, the cell adhesion of several adherence cells was observed using a field emission scanning electron microscope (FESEM). Due to its important role in controlling the morphology of the nanotube structures, extending anodization time causes the length of the nanotubes increases. Hence, the optimised anodization time on the TNA surface at 30 V with a sweep rate of 1 V/s directly impacts cell adhesion after incubating for 48 h. The anodic potential of TNA was successfully obtained at 30 V with a sweep rate of 1 V/sec for 30 min, which could modulate diverse cellular responses of cell adhesion observed by FESEM.

Mechano‐cytoskeleton remodeling mechanism and molecular docking studies on nanosurface technology: Titania nanotube arrays

“…8,9 TNA have been described previously with unique surface roughness properties, contact angle, and surface energy that is favorable for cell interaction. [10][11][12][13][14][15] Studies have reported that TNA nanosurface provides better cell spreading and differentiation behavior. Most notably, this study has provided insights into understanding the cell-TNA mechanotransduction response.…”

“…TNA have been described previously with unique surface roughness properties, contact angle, and surface energy that is favorable for cell interaction 10–15 . Studies have reported that TNA nanosurface provides better cell spreading and differentiation behavior.…”