In this research work, we explore the UV-protection and antibacterial activity of cotton fabric functionalized with copper oxide- titanium oxide nano composites (CuO@TiO2 NCs). The cotton fabrics based textile modified with L-methionine as cross-linking agents, by padding and rolling technique at higher temperature, and after, in situ synthesis and immobilization of the CuO@TiO2 NCs carried out using cupper nitrate (Cu(NO3)2 and titanium isopropoxide (TIP) as a source of Cu(OH)2 and TiO2 colloid which further converting into CuO and TiO2 nanoparticles (NPs) respectively to prepare UV-protective and antibacterial cotton fabrics. By this method 18.09 nm CuO and 32.5 nm TiO2 NPs of average crystal size were prepared respectively. The morphology and structure of the textile samples were characterized using FTIR, Raman Spectroscopy, High resolution scanning electron microscopy (HR-SEM), Energy-dispersive X-ray spectroscopy (EDS), X-ray photo spectroscopy (XPS), thermal gravimetric analysis (TGA) and XRD. The optical properties of the cotton fabric based textile samples were discussed using UV-vis diffuse reflectance spectrum (UV-DRS), and the antibacterial activity, ultraviolet protection performance, water absorption and vapor permeability of the cotton fabric based textile samples were analyzed. The results showed that the carboxyl groups of L-methionine were esterified with hydroxyl groups of the cotton fabric and the amine and the thiol ether group formed on the surface of the cotton fabrics which further enhance the binding of NPs. CuO@TiO2 NCs were successfully loaded onto the cotton fabrics by strong electrostatic interaction, causing the outstanding antibacterial activity against gram positive bacterial Staphylococcus aureus and gram negative bacteria E.coli. In addition, compared with the unfunctionalized cotton fabrics, the UV protection factor reached 50+, thus, multifunctional cotton fabrics with excellent antibacterial and anti-ultraviolet properties were obtained.