Titanium Surface Properties Influence the Biological Activity and FasL Expression of Craniofacial Stromal Cells

Abstract

Mesenchymal stromal cells (MSCs) can be easily isolated form craniofacial bones during routine dentistry procedures. Due to their embryological origin from neural crest, they represent a suitable cell population to study cell-biomaterial interaction in the craniofacial field, including osteoinductive/osteointegrative processes. The biological and immunomodulatory properties of MSCs may be influenced by chemistry and topography of implant surfaces. We investigated if and how three different titanium surfaces, machined (MCH), sandblasted with resorbable blasting medium (RBM), and Ca++-nanostructured (NCA), may affect biological activity, osseointegration, and immunomodulatory properties of craniofacial MSCs. Cell proliferation, morphology, osteogenic markers, and FasL were evaluated on MSCs isolated from the mandibular bone after seeding on these three different surfaces. No statistically significant differences in cell proliferation were observed whereas different morphologies and growth patterns were detected for each type of surface. No difference in the expression of osteogenic markers was revealed. Interestingly, FasL expression, involved in the immunomodulatory activity of stem cells, was influenced by surface properties. Particularly, immunofluorescence analysis indicated that FasL expression increased on MCH surface compared to the others confirming the suggested role of FasL in promoting osteogenic differentiation. Titanium surface treatments and topography might reflect different biological behaviours of craniofacial MSCs and influence their osseointegration/immunomodulation properties.

Effects of a Novel Bioactive Glass Composition on Biological Properties of Human Dental Pulp Stem Cells

“…DABCO (Sigma Aldrich, Saint Louis, MO, USA) was used as an anti-fading mounting medium. Cell proliferation and morphology were assessed by using confocal microscopy (Nikon A1 confocal laser scanning microscope), as formerly described by Conserva et al [ 51 ]. Cell proliferation was evaluated by counting the DAPI-stained nuclei on 10 randomly selected fields measuring 2.85 × 10 5 μm 2 for each disk by an individual blinded to the experimental details.…”

Modulation of Cell Death and Promotion of Chondrogenic Differentiation by Fas/FasL in Human Dental Pulp Stem Cells (hDPSCs)

“…Moreover, areas with the strongest colocalized signals, corresponding to pixels for both detectors, were selected to generate colocalization binary maps. In order to evaluate the expression of the typical mesenchymal stem cells (MSCs) markers, immune-selected hDPSCs at passage 1 underwent FACS analysis against CD73, CD90, CD105, CD34, CD45, HLA-DR, as formerly described by Conserva et al (2019). Following trypsin dissociation, cells were resuspended in culture medium and were stained with the following fluorochrome-conjugated antibodies (Abs): anti-human-CD73-PE-CY7, -CD90-FITC, -CD105-APC, -CD45-PE, and -HLA-DR-PE-CY7 (all from BD Biosciences, Franklin Lakes, NJ, United States); and -CD34-ECD (Beckman Coulter, Fullerton, CA, United States).…”

“…To evaluate chondrogenic differentiation, cell pellets from each experimental group were embedded in paraffin and 5 µm thick sections were obtained by microtome. Histological analysis by using Alcian Blue and Masson's trichrome staining were performed (Pisciotta et al, 2015b;Conserva et al, 2019).…”

Enhanced VEGF/VEGF-R and RUNX2 Expression in Human Periodontal Ligament Stem Cells Cultured on Sandblasted/Etched Titanium Disk

“…Owing to their embryological origin from neural crest, they appear as a suitable cell population to evaluate cell-biomaterial connection in the craniofacial field, involving osteoinductive/osteointegrative events. The biological and immunomodulatory characteristics of MSCs could be affected by chemistry and topography of implant surfaces (Conserva et al, 2019). Until now, six different human dental stem cells have been reported in the literature: human dental pulp stem cells (DPSCs), human exfoliated deciduous teeth stem cells (SHED), hPDLSCs (Sinjari et al, 2019), human apical papilla stem cells (APSCs), human dental follicle stem cells (DFSCs), and human gingival GMSCs (Romeo et al, 2018;Trubiani et al, 2019b).…”