TLC analysis of some phenolic compounds in kombucha beverage

Malbaša, Radomir V.; Lončar, Eva S.; Kolarov, Ljiljana A.

doi:10.2298/apt0435199m

Cited by 9 publications

(8 citation statements)

References 6 publications

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“…The same solvent system was used to separate phenolic compounds from kombucha beverages. 6 Alkaloids and flavonoids have also been reported from the root of M. jalapa. 13…”

Section: Discussionmentioning

confidence: 99%

“…The following six different solvent systems were used as developing systems: hexane:ethyl acetate:methanol (5:3:2); benzene:methanol (45:55); chloroform:methanol:acetic acid:water (5:4:0.2:1); 1-butanol:acetic acid:water (4:1:5); petroleum ether:chloroform:methanol for terpenoids; and chloroform:ethyl acetate:formic acid for flavonoids. 5 , 6 The plates were air dried and visualized by spraying with vanillin–sulfuric acid spray followed by heating for 5 minutes at 110°C for detection of steroids and triterpenoids. 7 Flavonoids were detected under ultraviolet rays at 365 nm.…”

Section: Methodsmentioning

confidence: 99%

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Isolation and characterization of bioactive components from Mirabilis jalapa L. radix

Gogoi

Nakhuru

Policegoudra

et al. 2016

Journal of Traditional and Complementary Medicine

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The present investigation was carried out to isolate and characterize bioactive components from Mirabilis jalapa L. radix (紫茉莉根 zǐ mò lì gēn). Thin-layer chromatography was used for the separation of spots from fractions of the crude extract. Separated spots were collected for identification of their activities. Free-radical scavenging activity was evaluated by spraying thin-layer chromatography plates (spotted with fractions) with 0.2% of 2,2-diphenyl-1-picrylhydrazyl solution. Activity against human pathogens such as Staphylococcus aureus and Candida albicans were determined using the agar diffusion method. Potential spots were subjected to infrared (IR) analysis and gas chromatography for characterization. Two spots (5F1 and 1F3) showed free-radical scavenging activity. The 1F3 spot was active against both S. aureus and C. albicans, whereas the 5F1 spot was active against S. aureus only. IR spectral analysis indicated that 5F1 spot to be a triterpenoid. Using IR spectral analysis and an IR library search, the 1F3 spot was identified to be a flavone, which may have a hydroxyl group in ring “A” of the flavone nucleus. Our results indicated that the 1F3 and 5F1 spots are potential free-radical scavengers. Both 1F3 and 5F1 exhibited antimicrobial activity. IR spectral analysis coupled with an IR library search indicated 1F3 and 5F1 to be a flavone and a triterpenoid, respectively.

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“…The same solvent system was used to separate phenolic compounds from kombucha beverages. 6 Alkaloids and flavonoids have also been reported from the root of M. jalapa. 13…”

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Isolation and characterization of bioactive components from Mirabilis jalapa L. radix

Gogoi

Nakhuru

Policegoudra

et al. 2016

Journal of Traditional and Complementary Medicine

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“…The Thin layer chromatography (TLC) performed for all five extracts on analytical plates over silica gel-G of 0.2 mm thickness. These plates were developed in three different mobile phases as described in literatures and their composition is listed in Table 3 13,14 .…”

Section: Tlc Analysis Of Leaves Extracts Of Jasminum Multiflorummentioning

confidence: 99%

Untitled

2013

IJPSR

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Jasminum multiflorum belongs to the Oleaceae family. It is used as a traditional medicine from ancient times. It is a large evergreen twinner shrub found throughout India. Present study reports the phytochemical properties which have been carried out on the leaves extract using ethanol, methanol, ethyl acetate, acetone and n-hexane. The phytochemicals analysis show the presence of Phenols, Saponins, Terpenoids etc; TLC has been carried out on different extracts of leaves of Jasminum multiflorum, which have shown different R f values. The presences of phenols, terpenoids and saponins based phytochemicals are reported.

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“…Efficient detection and rapid characterization of natural products play an important role as an analytical support in the work of natural products chemists. Existing methods for the analysis of phenolic compounds involved high-performance liquid chromatography (HPLC) (Weisz et al, 2009;Proestos et al, 2005), thin layer chromatography (TLC) (Malbaša et al, 2004), capillary electrophoresis (CE) (Hinneburg et al, 2004;Peng et al, 2005), and gas chromatography (GC) (Du et al, 2009). Among several chromatographic methods, HPLC is the most widely used technique for both qualitative and quantitative analysis of phytochemical substance.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous analysis of eight phenolic compounds in Phyllanthus simplex Retz by HPLC-DAD-ESI/MS

Aboul‐Enein¹,

El-Ela²,

Shalaby³

et al. 2012

J. Med. Plants Res.

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A new, simple and sensitive high-performance liquid chromatography-diode array detector (HPLC-DAD) method was developed for the simultaneous determination of eight phenolic components in Phyllanthus simplex Retz. The chemical profile of the eight components, including gallic acid, caffeic acid, quercetin, ferulic acid, luteolin-7-O-glucoside, 8,9-epoxy brevifolin, brevifolin and globulariacitrin were acquired by using high-performance liquid chromatography-diode array detector coupled to an electrospray ionization mass spectrometer (HPLC-DAD-ESI/MS). The optimal condition of separation was achieved on a ZORBAX Extend C 18 column (5 µm, 250×4.6 mm i.d.) with a gradient elution of methanol and 1% acetic acid at a flow rate of 1.0 ml/min. Under ultra violent (UV) detection at 275 nm, the recoveries of the analytes were in the range of 99.2 to 99.8% with a RSD of 0.41 to 1.50%. The limits of detection and quantification for the analytes were ranged from 55 to 140 ng/ml and from 155 to 320 ng/ml, respectively. The intra-day RSD% ranged from 0.32 to 0.82% and the inter-day RSD% were not higher than 5.0%. The proposed method is suitable for quantitative and qualitative determination of the eight bioactive phenolic components in P. simplex Retz.

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TLC analysis of some phenolic compounds in kombucha beverage

Cited by 9 publications

References 6 publications

Isolation and characterization of bioactive components from Mirabilis jalapa L. radix

Isolation and characterization of bioactive components from Mirabilis jalapa L. radix

Untitled

Simultaneous analysis of eight phenolic compounds in Phyllanthus simplex Retz by HPLC-DAD-ESI/MS

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