Isolation and characterization of bioactive components from Mirabilis jalapa L. radix

Gogoi, Jyotchna; Nakhuru, Khonamai Sewa; Policegoudra, R.S.; Chattopadhyay, Pronobesh; Kumar, Ashok; Veer, Vijay

doi:10.1016/j.jtcme.2014.11.028

Cited by 32 publications

(32 citation statements)

References 8 publications

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“…The presence of these bioactive compounds as observed in this study conferred preservative property on the leaf extract of M. macrostachyum hence the activity of plant extracts can be ascribed to the presence of antifungal agents that hinder the growth of the test fungi (Martins et al, 2011;Gogoi et al, 2016). The qualitative phytochemical screening of the extracts from the leaf of M. macrostachyum revealed the presence of alkaloids, tannins, saponins, flavonoids, steroids and terpenoids.…”

Section: Discussionsupporting

confidence: 59%

Chemical Composition, Toxicity and Antifungal Activities of Megaphrynium macrostachyum (K. Schum) Leaf Extract against Foodborne Fungi

Adeogun¹,

Adekunle²,

Adongbede³

et al. 2017

Not Sci Biol

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This study aimed to examine the preservative potential of Megaphrynium macrostachyum on fungi responsible for the deterioration of orange juice and corn Jell-O. The phytochemicals from plants' leaves were extracted with four solvents: acetone, aqueous, ethanol and hexane. The solvents were differently and tested against fungi isolated from orange juice and corn Jell-O using disc diffusion method. Phytochemical screening of the extracts from the leaves was carried out, and the most active extract was tested via GC-MS for the essential oils and HPLC fingerprinting. The toxicity test of the extracts against brine shrimp was carried out after exposure for 24 hours. The toxicity test showed that the extracts were non-toxic on the Brine Shrimps at LC 50 (379.21μg/ml and 107.21μg/ml for aqueous and ethanol extracts). The qualitative phytochemical test reported the presence of alkaloids, tannins, saponins, flavonoids, steroids and terpenoids in different extracts of the plant' leaves. The quantitative phytochemical determination of the most active extract revealed alkaloids with the highest contents of 107.48 mg/100 g. The GC-MS analyses of the fresh leaves of the plants revealed the presence of isodecane with the highest percentage at 15.56%. The GC-MS analyses of the dried leaves revealed isodecane with the highest percentage at 10.43%. The HPLC analysis revealed the presence of various phytochemical constituents in the dried leaves. This study has been able to establish the potency of Megaphrynium macrostachyum leaves on fungi associated with the spoilage of Citrus sinensis (orange) juice and Corn Jell-O ('Eko') which contribute to tremendous research towards the use and acknowledgment of natural antimicrobials for the preservation of food.

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Section: Discussionsupporting

confidence: 59%

Chemical Composition, Toxicity and Antifungal Activities of Megaphrynium macrostachyum (K. Schum) Leaf Extract against Foodborne Fungi

Adeogun¹,

Adekunle²,

Adongbede³

et al. 2017

Not Sci Biol

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“…Mirabilis jalapa (Nyctaginaceae) commonly known as “four o’clock plant” and “Marvel of Peru”, is an ornamental flowering plant. The plant is been used as traditional medicine throughout the world for the treatment of various diseases and disorders including sexually transmitted diseases, urinary and kidney infections, acute arthritis, anesthesia, inflammation, irregular menstruation and cancerous growths [ 20 – 24 ]. The phytochemical constituents and medicinal property of this plant are well characterized and rich in many active compounds including triterpenes, flavonoids, alkaloids, and steroids [ 20 , 24 ].…”

Section: Introductionmentioning

confidence: 99%

“…The plant is been used as traditional medicine throughout the world for the treatment of various diseases and disorders including sexually transmitted diseases, urinary and kidney infections, acute arthritis, anesthesia, inflammation, irregular menstruation and cancerous growths [ 20 – 24 ]. The phytochemical constituents and medicinal property of this plant are well characterized and rich in many active compounds including triterpenes, flavonoids, alkaloids, and steroids [ 20 , 24 ]. For example, an antiviral ribosome-inactivating protein (RIP) and rotenoids showed an effective inhibition of HIV-1 reverse transcriptase activity isolated from Mirabilis jalapa [ 22 , 25 ].…”

Section: Introductionmentioning

confidence: 99%

Determination and production of antimicrobial compounds by Aspergillus clavatonanicus strain MJ31, an endophytic fungus from Mirabilis jalapa L. using UPLC-ESI-MS/MS and TD-GC-MS analysis

et al. 2017

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Endophytic fungi associated with medicinal plants are reported as potent producers of diverse classes of secondary metabolites. In the present study, an endophytic fungi, Aspergillus clavatonanicus strain MJ31, exhibiting significant antimicrobial activity was isolated from roots of Mirabilis jalapa L., was identified by sequencing three nuclear genes i.e. internal transcribed spacers ribosomal RNA (ITS rRNA), 28S ribosomal RNA (28S rRNA) and translation elongation factor 1- alpha (EF 1α). Ethyl acetate extract of strain MJ31displayed significant antimicrobial potential against Bacillus subtilis, followed by Micrococccus luteus and Staphylococcus aureus with minimum inhibitory concentrations (MIC) of 0.078, 0.156 and 0.312 mg/ml respectively. In addition, the strain was evaluated for its ability to synthesize bioactive compounds by the amplification of polyketide synthase (PKS) and non ribosomal peptide synthetase (NRPS) genes. Further, seven antibiotics (miconazole, ketoconazole, fluconazole, ampicillin, streptomycin, chloramphenicol, and rifampicin) were detected and quantified using UPLC-ESI-MS/MS. Additionally, thermal desorption-gas chromatography mass spectrometry (TD-GC-MS) analysis of strain MJ31 showed the presence of 28 volatile compounds. This is the first report on A. clavatonanicus as an endophyte obtained from M. jalapa. We conclude that A. clavatonanicus strain MJ31 has prolific antimicrobial potential against both plant and human pathogens and can be exploited for the discovery of new antimicrobial compounds and could be an alternate source for the production of secondary metabolites.

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“…However, the results of phytochemical screening in our study were not positive for steroids. This could perhaps be because our extract comprised flowers, leaves, and tubers of M. jalapa L., whereas the extracts mentioned in the previous studies could have contained different components or concentrations of M. jalapa L. Furthermore, our extract was autoclaved at 121°C before use, and this could have possibly altered the stability of any steroid phytotoxins that were present [10][11][12][13]. Table 4, the highest mean wound reduction percentage at day 13 was by dose 3 (97.42%) followed by that by dose 1, the positive…”

Section: Discussionmentioning

confidence: 99%

Effect of the Water Extract of the Four O’clock Herb (Mirabilis Jalapa L.) on the Healing of Open Wounds in Rats

Puspasari

Saputri

2018

Int J App Pharm

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Objective: This study aimed to analyze the effect on wound healing following treatment with a water extract of Mirabilis jalapa L. by investigating wound contraction and the associated histopathological changes in rat skin. Methods:Male Sprague-Dawley rats were divided into five groups, namely negative control, positive control (povidone-iodine), dose 1, dose 2, and dose 3. A 20-×10-mm rectangular wound area was created for the test. In dose 1, 2, and 3 groups, the corresponding dose variation of a 0.5-mL M. jalapa L. water extract (dose 1: 5% v/v, ≈243.1 mg/kg body weight BW; dose 2: 10% v/v, ≈486.2 mg/kg BW; and dose 3: 20% v/v, ≈972.4 mg/kg BW) was topically applied for 14 days on open wounds of rats. Widespread wound contractions were measured on days 1, 3, 5, 7, 9, 11, and 13, and histopathological changes in the skin were observed on day 15 using hematoxylin and eosin staining.Results: The M. jalapa L. water extract accelerated wound healing. The optimal dose was found to be 20% v/v (≈972.4 mg/kg BW). Conclusion: M. jalapa L. extracts are potential healing agents for open wounds. Puspasari and Saputri contraction parameters and histopathological indicators. An effective dose of 972.4 mg/kg body weight was determined.

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Isolation and characterization of bioactive components from Mirabilis jalapa L. radix

Cited by 32 publications

References 8 publications

Chemical Composition, Toxicity and Antifungal Activities of Megaphrynium macrostachyum (K. Schum) Leaf Extract against Foodborne Fungi

Chemical Composition, Toxicity and Antifungal Activities of Megaphrynium macrostachyum (K. Schum) Leaf Extract against Foodborne Fungi

Determination and production of antimicrobial compounds by Aspergillus clavatonanicus strain MJ31, an endophytic fungus from Mirabilis jalapa L. using UPLC-ESI-MS/MS and TD-GC-MS analysis

Effect of the Water Extract of the Four O’clock Herb (Mirabilis Jalapa L.) on the Healing of Open Wounds in Rats

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