TLC Screening for Antioxidant Activity of Extracts from Fifteen Bamboo Species and Identification of Antioxidant Flavone Glycosides from Leaves of Bambusa. textilis McClure

Wang, Jin; Yue, Yongde; Tang, Feng; Sun, Jia

doi:10.3390/molecules171012297

Cited by 78 publications

(46 citation statements)

References 21 publications

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“…Glucoraphanin, precursor of the most active antioxidant glucosinolate found in broccoli, was assessed as the prevalent compound: 14.06 to 24.17 µ mol/g [116]. HPLC chromatographic assay of the methanolic extract of Bambusa textilis McClure indicated active antiradical fractions, as presented in Figure 1 [87]. …”

Section: Significant Analytical Applications To Plant and Plant Exmentioning

confidence: 99%

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Antioxidant Capacity Determination in Plants and Plant‐Derived Products: A Review

Pisoschi

Cîmpeanu

et al. 2016

Oxidative Medicine and Cellular Longevity

222

149

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The present paper aims at reviewing and commenting on the analytical methods applied to antioxidant and antioxidant capacity assessment in plant-derived products. Aspects related to oxidative stress, reactive oxidative species' influence on key biomolecules, and antioxidant benefits and modalities of action are discussed. Also, the oxidant-antioxidant balance is critically discussed. The conventional and nonconventional extraction procedures applied prior to analysis are also presented, as the extraction step is of pivotal importance for isolation and concentration of the compound(s) of interest before analysis. Then, the chromatographic, spectrometric, and electrochemical methods for antioxidant and antioxidant capacity determination in plant-derived products are detailed with respect to their principles, characteristics, and specific applications. Peculiarities related to the matrix characteristics and other factors influencing the method's performances are discussed. Health benefits of plants and derived products are described, as indicated in the original source. Finally, critical and conclusive aspects are given when it comes to the choice of a particular extraction procedure and detection method, which should consider the nature of the sample, prevalent antioxidant/antioxidant class, and the mechanism underlying each technique. Advantages and disadvantages are discussed for each method.

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Section: Significant Analytical Applications To Plant and Plant Exmentioning

confidence: 99%

“…HPLC chromatogram (at 330 nm) of the methanolic extract of Bambusa , with illustration of the antioxidant fractions F1, F2, F9, and F11 [87]. …”

Section: Figurementioning

confidence: 99%

Antioxidant Capacity Determination in Plants and Plant‐Derived Products: A Review

Pisoschi

Cîmpeanu

et al. 2016

Oxidative Medicine and Cellular Longevity

222

149

View full text Add to dashboard Cite

show abstract

“…An aliquot of aqueous solution of the nine medicinal herbs studies (40 μL) was directly deposited (as spots or bands) onto silica gel 60F254 TLC plates (Merck, Germany) (20). TLC plates were developed to a distance of 75 mm, in a 20 cm 20 cm glass flat-bottom chamber after equilibration with mobile phase vapor for 30 min with ethyl acetateformic acid-acetic acid-water (100:11:11:26) as developing reagents.…”

Section: Tlc Bioautography Methodsmentioning

confidence: 99%

Culinary Decoctions: Spectrophotometric Determination of Various Polyphenols Coupled with their Antioxidant Activities

Babili¹,

Babili²,

Souchard³

et al. 2013

TOPHARMCJ

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Abstract:The aqueous extracts of sweet basil (Ocimum basilicum L.), tarragon (Artemisia dracunculus L.), fennel (Foeniculum vulgare Miller), olive (Olea europaea L.), sage (Salvia officinalis L.), thyme (Thymus vulgaris L.), wild thyme (T. serpyllum L.), tea (Camellia sinensis (L.) Kuntze), and verbena (Verbena officinalis L.) were investigated for polyphenol, tannins, antocyanins and flavonoids. Among the nine culinary herbs, tea and wild thyme extracts contained the higher phenol content of 874.10 ± 3.50 GAE [g gallic acid equivalents/Kg (dry wt.) extract] and 945.70 ± 0.81 GAE, respectively, while the fennel extract contained the lowest content at 149.90 ± 1.16 GAE. Sage and verbena extracts do not contain any anthocyanins and have low levels of tannins (2.40 ± 0.12 and 1.97± 0.06 CE, respectively). Tea rich in tannins and verbena and sage, totally devoid of anthocyanins, have higher antioxidant activities according to the ABTS/DPPH assays. However, the results fail to show any positive correlation between phenol contents and antioxidant activities.

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“…This method prescribed on which concentration prepared showed highest intensity of white spot capacity on the TLC plate. It was an eye-detected semiquantitatively rapid screening method and only based on the inhibition of the accumulation of oxidized products [21] and the white colour represented the nature of radical scavenger presented in selected concentration of extract [12].…”

Section: Semi-quantitative Analysis Of Antioxidant Activity: Dpph Stamentioning

confidence: 99%

Comparative Study of Antioxidant and Antimicrobial Activity of Root, Stem and Leaves of Leea Indica Species

Harun¹,

Rahim²,

Jalil³

et al. 2016

MJS

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In this paper, we investigate the potency of Leea indica locally known as Memali plant from Tasik Chini, Pahang as a potent antioxidant and antimicrobial agent. Antibacterial activity of root, stem and leaf extracts of L. indica was evaluated against skin disease caused-microb Staphylococcus aureus and Staphylococcus epidermidis. Antioxidant activity was assessed by 2,2 diphenyl-1-picrylhydrazyl ( DPPH) thin layer chromatography (TLC) and dot blot assay method. The dichloromethane (DCM) extracts of all three parts displayed obvious antibacterial effect compared to other extracts and DCM extracts from leaves was the most effective in inhibiting the growth of S. epidermis with inhibition zone (IZ) of 18 mm. The methanol extracts from stem scavenged DPPH radicals more effective than root and leaf extracts at a concentration as low as 0.19 mg/ml. The antibacterial and antioxidant properties of leaves and stem might be attributed to the high content of terpenoid and phenolic. Thus, Memali appears to be a promising source of bioactive ingredients with antioxidant and antimicrobial activity. Keywords: Leea indica, antimicrobial, antioxidant, phytochemicals, secondary metabolitesAbstrak Kertas kerja ini meliputi penyelidikan ke atas potensi pokok Leea indica dari Tasik Chini, Pahang yang juga dipanggil Memali sebagai agen antioksidan dan antimikrob. Bahagian akar, batang dan daun dipilih untuk kajian aktiviti antibakteria dan antioksidan di mana ujian antibakteria menggunakan bakteria penyakit kulit seperti Staphylococcus aureus and Staphylococcus epidermidis. Ujian antioksidan menggunakan kaedah kromatografi lapisan nipis 2,2 diphenyl-1-picrylhydrazyl (DPPH) dan ujian dot blot. Didapati ekstrak dari diklorometana (DCM) menunjukkan aktiviti antimikrob yang lebih tinggi berbanding dengan ekstrak lain dan ekstrak dari daun mempunyai aktiviti yang paling tinggi dengan zon perencatan 18 mm. Ujian antioksidan menunujukkan ekstrak metanol bahagian batang lebih efektif memerangkap radikal DPPH pada kepekatan serendah 0.19 mg/ml. Sifat antioksidan dan antimikrob ini berkemungkinan akibat daripada adanya kandungan bahan seperti terpen dan fenolik yang tinggi. Maka pokok Memali dilihat sebagai punca asas bahan bioaktif yang bersifat antimikrob dan antioksidan.

show abstract

TLC Screening for Antioxidant Activity of Extracts from Fifteen Bamboo Species and Identification of Antioxidant Flavone Glycosides from Leaves of Bambusa. textilis McClure

Cited by 78 publications

References 21 publications

Antioxidant Capacity Determination in Plants and Plant‐Derived Products: A Review

Antioxidant Capacity Determination in Plants and Plant‐Derived Products: A Review

Culinary Decoctions: Spectrophotometric Determination of Various Polyphenols Coupled with their Antioxidant Activities

Comparative Study of Antioxidant and Antimicrobial Activity of Root, Stem and Leaves of Leea Indica Species

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