TMT based deep proteome analysis of buffalo mammary epithelial cells and identification of novel protein signatures during lactogenic differentiation

Jaswal, Shalini; Anand, Vijay; Ali, Syed Azmal; Jena, Manoj Kumar; Kumar, Sanjay; Kaushik, Jai K.; Mohanty, Ashok Kumar

doi:10.1096/fj.202002476rr

Cited by 10 publications

(5 citation statements)

References 78 publications

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“…The extensive proliferation of MECs is followed by their terminal differentiation during the late stages of pregnancy. To understand the protein machinery governing the differentiation in buffalo mammary epithelial cells (BuMECs), a differential proteome study was performed by Jaswal et al (2021) [ 57 ]. The tandem mass tag (TMT)-based differential proteome analysis of hormone-treated (prolactin, 8 µg/mL; bovine insulin, 1 µg/mL; and hydrocortisone, 1 µg/mL) BuMECs at different time points of 3, 6, 12, and 15 days identified a total of 4934 proteins; of them, 681 were differentially regulated.…”

Section: Structural and Functional Development Of The Bovine Mammary ...mentioning

confidence: 99%

Critical Review on Physiological and Molecular Features during Bovine Mammary Gland Development: Recent Advances

Jaswal

Jena

Anand

et al. 2022

Cells

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The mammary gland is a unique organ with the ability to undergo repeated cyclic changes throughout the life of mammals. Among domesticated livestock species, ruminants (cattle and buffalo) constitute a distinct class of livestock species that are known milk producers. Cattle and buffalo contribute to 51 and 13% of the total milk supply in the world, respectively. They also play an essential role in the development of the economy for farming communities by providing milk, meat, and draft power. The development of the ruminant mammary gland is highly dynamic and multiphase in nature. There are six developmental stages: embryonic, prepubertal, pubertal, pregnancy, lactation, and involution. There has been substantial advancement in our understanding of the development of the mammary gland in both mouse and human models. Until now, there has not been a thorough investigation into the molecular processes that underlie the various stages of cow udder development. The current review sheds light on the morphological and molecular changes that occur during various developmental phases in diverse species, with a particular focus on the cow udder. It aims to explain the physiological differences between cattle and non-ruminant mammalian species such as humans, mice, and monkeys. Understanding the developmental biology of the mammary gland in molecular detail, as well as species-specific variations, will facilitate the researchers working in this area in further studies on cellular proliferation, differentiation, apoptosis, organogenesis, and carcinogenesis. Additionally, in-depth knowledge of the mammary gland will promote its use as a model organ for research work and promote enhanced milk yield in livestock animals without affecting their health and welfare.

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Section: Structural and Functional Development Of The Bovine Mammary ...mentioning

confidence: 99%

Critical Review on Physiological and Molecular Features during Bovine Mammary Gland Development: Recent Advances

Jaswal

Jena

Anand

et al. 2022

Cells

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“…On the other hand, genome editing might be utilised to precisely knock-in valuable alleles (such as heat tolerance, illness resistance), as well as haplotypes into our native locally well-adapted cattle breeds genome, subsequently to improve their productivity [ 90 ]. We recently used the buffalo mammary epithelial cells to understand lactogenic signalling [ 91 , 92 ].…”

Section: Genome Editing In Ruminants Such As Cattle and Buffalosmentioning

confidence: 99%

Impact of CRISPR-Cas9-Based Genome Engineering in Farm Animals

Singh

Ali

2021

Veterinary Sciences

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Humans are sorely over-dependent on livestock for their daily basic need of food in the form of meat, milk, and eggs. Therefore, genetic engineering and transgenesis provide the opportunity for more significant gains and production in a short span of time. One of the best strategies is the genetic alteration of livestock to enhance the efficiency of food production (e.g., meat and milk), animal health, and welfare (animal population and disease). Moreover, genome engineering in the bovine is majorly focused on subjects such as disease resistance (e.g., tuberculosis), eradicate allergens (e.g., beta-lactoglobulin knock-out), products generation (e.g., meat from male and milk from female), male or female birth specifically (animal sexing), the introduction of valuable traits (e.g., stress tolerance and disease resistance) and their wellbeing (e.g., hornlessness). This review addressed the impressive genome engineering method CRISPR, its fundamental principle for generating highly efficient target-specific guide RNA, and the accompanying web-based tools. However, we have covered the remarkable roadmap of the CRISPR method from its conception to its use in cattle. Additionally, we have updated the comprehensive information on CRISPR-based gene editing in cattle.

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“…However, the immune response is not obviously linked to other observations. TTLL12 is: a target for serum autoantibodies after acute angle closure glaucoma attack [28], a candidate gene for triglycerides in mice [29], and a gene that is differently expressed during lactogenic differentiation of buffalo mammary epithelial cells [30]. Interestingly, TTLL12 is required for the formation of primary ciliary axonemes, which are critical sensory organelles in polarized epithelial cells [12].…”

Section: Introductionmentioning

confidence: 99%

TTLL12 has a potential oncogenic activity, suppression of ligation of nitrotyrosine to the C-terminus of detyrosinated α-tubulin, that can be overcome by molecules identified by screening a compound library

Deshpande,

Brants,

Wasylyk

et al. 2024

PLoS ONE

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Tubulin tyrosine ligase 12 (TTLL12) is a promising target for therapeutic intervention since it has been implicated in tumour progression, the innate immune response to viral infection, ciliogenesis and abnormal cell division. It is the most mysterious of a fourteen-member TTL/TTLL family, since, although it is the topmost conserved in evolution, it does not have predicted enzymatic activities. TTLL12 seems to act as a pseudo-enzyme that modulates various processes indirectly. Given the need to target its functions, we initially set out to identify a property of TTLL12 that could be used to develop a reliable high-throughput screening assay. We discovered that TTLL12 suppresses the cell toxicity of nitrotyrosine (3-nitrotyrosine) and its ligation to the C-terminus of detyrosinated α-tubulin (abbreviated to ligated-nitrotyrosine). Nitrotyrosine is produced by oxidative stress and is associated with cancer progression. Ligation of nitrotyrosine has been postulated to be a check-point induced by excessive cell stress. We found that the cytotoxicities of nitrotyrosine and tubulin poisons are independent of one another, suggesting that drugs that increase nitrotyrosination could be complementary to current tubulin-directed therapeutics. TTLL12 suppression of nitrotyrosination of α-tubulin was used to develop a robust cell-based ELISA assay that detects increased nitrotyrosination in cells that overexpress TTLL12 We adapted it to a high throughput format and used it to screen a 10,000 molecule World Biological Diversity SETTM collection of low-molecular weight molecules. Two molecules were identified that robustly activate nitrotyrosine ligation at 1 μM concentration. This is the pioneer screen for molecules that modulate nitrotyrosination of α-tubulin. The molecules from the screen will be useful for the study of TTLL12, as well as leads for the development of drugs to treat cancer and other pathologies that involve nitrotyrosination.

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TMT based deep proteome analysis of buffalo mammary epithelial cells and identification of novel protein signatures during lactogenic differentiation

Cited by 10 publications

References 78 publications

Critical Review on Physiological and Molecular Features during Bovine Mammary Gland Development: Recent Advances

Critical Review on Physiological and Molecular Features during Bovine Mammary Gland Development: Recent Advances

Impact of CRISPR-Cas9-Based Genome Engineering in Farm Animals

TTLL12 has a potential oncogenic activity, suppression of ligation of nitrotyrosine to the C-terminus of detyrosinated α-tubulin, that can be overcome by molecules identified by screening a compound library

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