Tn<i>10</i>/IS<i>10</i> transposition is downregulated at the level of transposase expression by the RNA‐binding protein Hfq

Ross, Joseph A.; Wardle, Simon J.; Haniford, David B.

doi:10.1111/j.1365-2958.2010.07359.x

Cited by 34 publications

(60 citation statements)

References 59 publications

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“…Importantly, the increase in transposase expression was observed only in the situation where the reporter construct included both the native transposase promoter and sequences required for translational control. This is most consistent with Hfq acting as a posttranscriptional regulator of transposase expression (45). Roughly 20 years prior to the work described above a potent posttranscriptional regulatory system had been described for the Tn10 system.…”

Section: Regulation Of Tn10 and Tn5 Transposition By Hfq Backgroundsupporting

confidence: 53%

“…As DsrA is the mediator of posttranscriptional control of H-NS expression and Hfq is the accessory factor, it was inferred that the upregulation of Tn10 transposition seen in hfq deficiency was unrelated to increased H-NS protein levels. That is, Hfq acts independent of H-NS to downregulate Tn10 transposition (45). the possibility was tested that Hfq might be inhibiting Tn10 transposase expression.…”

Section: Regulation Of Tn10 and Tn5 Transposition By Hfq Backgroundmentioning

confidence: 99%

“…Consistent with this possibility it was found that the impact of disrupting the hfq gene on Tn10 transposition was greatly reduced (but not nullified) under conditions in which RNA-OUT was an ineffective inhibitor of transposase expression (i.e., when the transposon was present in the chromosome in a single copy). In addition, work in vitro demonstrated that Hfq could bind to both RNA-IN and RNA-OUT and accelerate the rate at which these molecules pair (45).…”

Section: Regulation Of Tn10 and Tn5 Transposition By Hfq Backgroundmentioning

confidence: 99%

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Transposons Tn 10 and Tn 5

Haniford

Ellis

2015

Microbiol Spectr

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The study of the bacterial transposons Tn10 and Tn5 has provided a wealth of information regarding steps in nonreplicative DNA transposition, transpososome dynamics and structure, as well as mechanisms employed to regulate transposition. The focus of ongoing research on these transposons is mainly on host regulation and the use of the Tn10 antisense system as a platform to develop riboregulators for applications in synthetic biology. Over the past decade two new regulators of both Tn10 and Tn5 transposition have been identified, namely H-NS and Hfq proteins. These are both global regulators of gene expression in enteric bacteria with functions linked to stress-response pathways and virulence and potentially could link the Tn10 and Tn5 systems (and thus the transfer of antibiotic resistance genes) to environmental cues. Work summarized here is consistent with the H-NS protein working directly on transposition complexes to upregulate both Tn10 and Tn5 transposition. In contrast, evidence is discussed that is consistent with Hfq working at the level of transposase expression to downregulate both systems. With regard to Tn10 and synthetic biology, some recent work that incorporates the Tn10 antisense RNA into both transcriptional and translational riboswitches is summarized.

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Section: Regulation Of Tn10 and Tn5 Transposition By Hfq Backgroundsupporting

confidence: 53%

Section: Regulation Of Tn10 and Tn5 Transposition By Hfq Backgroundmentioning

confidence: 99%

Section: Regulation Of Tn10 and Tn5 Transposition By Hfq Backgroundmentioning

confidence: 99%

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Transposons Tn 10 and Tn 5

Haniford

Ellis

2015

Microbiol Spectr

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“…7,9,[11][12][13] Nonetheless, Hfq has been shown to negatively regulate transposition in E. coli by promoting pairing of an asRNA to the IS10 transposase mRNA. 52,53 RNAseq-based transcriptomic profiling of S. meliloti has evidenced pervasive antisense transcription involving approximately 35% of the annotated protein-coding genes. 23 CoIP recovered only 2% of the annotated S. meliloti asRNAs, none of which transcribed opposite to genes encoding transposable elements, further supporting the presumably discrete influence of Hfq in asRNAmediated regulation of gene expression.…”

Section: Discussionmentioning

confidence: 99%

Genome-wide profiling of Hfq-binding RNAs uncovers extensive post-transcriptional rewiring of major stress response and symbiotic regulons inSinorhizobium meliloti

et al. 2014

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“…This suggests the potential involvement of sRNAs in inactivating transposition in bacteria (Landt et al 2008). The role of sRNAs regulation of transposition in E. coli was illustrated in an elegant study by Ross et al (2010). Hfq can be an indirect negative regulator of DsrAmediated transposition.…”

Section: Involvement Of Srnas In Mycobacterial Growth Metabolism Anmentioning

confidence: 98%

Identification of small RNAs in Mycobacterium smegmatis using heterologous Hfq

Qin

et al. 2012

RNA

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Gene regulation by small RNAs (sRNAs) has been extensively studied in various bacteria. However, the presence and roles of sRNAs in mycobacteria remain largely unclear. Immunoprecipitation of RNA chaperone Hfq to enrich for sRNAs is one of the effective methods to isolate sRNAs. However, the lack of an identified mycobacterial hfq restricts the feasibility of this approach. We developed a novel method that takes advantage of the conserved inherent sRNAs-binding capability of heterologous Hfq from Escherichia coli to enrich sRNAs from Mycobacterium smegmatis, a model organism for studying Mycobacterium tuberculosis. We validated 12 trans-encoded and 12 cis-encoded novel sRNAs in M. smegmatis. Many of these sRNAs are differentially expressed at exponential phase compared with stationary phase, suggesting that sRNAs are involved in the growth of mycobacteria. Intriguingly, five of the cis-encoded novel sRNAs target known transposases. Phylogenetic conservation analysis shows that these sRNAs are pathogenicity dependent. We believe that our findings will serve as an important reference for future analysis of sRNAs regulation in mycobacteria and will contribute significantly to the development of sRNAs prediction programs. Moreover, this novel method of using heterologous Hfq for sRNAs enrichment can be of general use for the discovery of bacterial sRNAs in which no endogenous Hfq is identified.

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Tn10/IS10 transposition is downregulated at the level of transposase expression by the RNA‐binding protein Hfq

Cited by 34 publications

References 59 publications

Transposons Tn 10 and Tn 5

Transposons Tn 10 and Tn 5

Genome-wide profiling of Hfq-binding RNAs uncovers extensive post-transcriptional rewiring of major stress response and symbiotic regulons inSinorhizobium meliloti

Identification of small RNAs in Mycobacterium smegmatis using heterologous Hfq

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