Tn-sequencing of Mycoplasma hyopneumoniae and Mycoplasma hyorhinis mutant libraries reveals non-essential genes of porcine mycoplasmas differing in pathogenicity

Trueeb, Bettina S.; Gerber, Simona; Maes, Dominiek; Gharib, Walid H.; Kuhnert, Peter

doi:10.1186/s13567-019-0674-7

Cited by 13 publications

(16 citation statements)

References 28 publications

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“…But the genetic operating system is still poorly studied for most of the mycoplasma species. In addition, DnaK plays an important role in bacterial growth and reproduction, which are predicted to be essential gene in M. hyorhinis ( Trueeb et al, 2019 ). Therefore, M. hyorhinis mutant missing DnaK gene seems to be unavailable.…”

Section: Discussionmentioning

confidence: 99%

DnaK Functions as a Moonlighting Protein on the Surface of Mycoplasma hyorhinis Cells

Wang

Liu

et al. 2022

Front. Microbiol.

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Mycoplasma hyorhinis is a common pathogen of swine and is also associated with various human tumors. It causes systemic inflammation, typically polyserositis and polyarthritis, in some infected pigs. However, the pathogenic mechanism of M. hyorhinis remains unclear. DnaK is a highly conserved protein belonging to the heat-shock protein 70 family of molecular chaperones, which plays important roles as a moonlighting protein in various bacteria. In the present study, we identified the surface exposure of M. hyorhinis DnaK. Two virulent strains expressed more DnaK on their surface than the avirulent strain. Thereafter, the potential moonlighting functions of DnaK were investigated. Recombinant M. hyorhinis DnaK (rMhr-DnaK) was found to be able to adhere to swine PK-15 cells and human NCI-H292 cells. It also bound to four extracellular matrix components—fibronectin, laminin, type IV collagen, and vitronectin—in a dose-dependent manner. ELISA demonstrated an interaction between rMhr-DnaK and plasminogen, which was significantly inhibited by a lysine analog, ε-aminocaproic acid. rMhr-DnaK-bound plasminogen was activated by tissue-type plasminogen activator (tPA), and the addition of rMhr-DnaK significantly enhanced the activation. Finally, a DnaK-specific antibody was detected in the serum of pigs immunized with inactivated vaccines, which indicated good immunogenicity of it. In summary, our findings imply that DnaK is an important multifunctional moonlighting protein in M. hyorhinis and likely participates extensively in the infection and pathogenesis processes of M. hyorhinis.

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Section: Discussionmentioning

confidence: 99%

DnaK Functions as a Moonlighting Protein on the Surface of Mycoplasma hyorhinis Cells

Wang

Liu

et al. 2022

Front. Microbiol.

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“…21 BLASTX v2.2.26 alignment was carried out for all the genomes assayed 22 All-versus-all BLASTP alignment (50% identity; e-value 1e-4 cutoff) was executed for protein sequences extracted from each strain. 23 BLASTP outputs were used as inputs to cluster proteins into families sharing the same function using the Markov Cluster Algorithm (MCL) with an inflation index of 2.5. 24 The gene families obtained were classified to the core genome or the dispensable genome, based on their existence in all or in a subset of the strains investigated.…”

Section: Bifidobacterial Genome Sequencing and Bioinformatics Analysismentioning

confidence: 99%

Development of gut microbiota and bifidobacterial communities of neonates in the first 6 weeks and their inheritance from mother

et al. 2021

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Microbiota especially Bifidobacterium play an important role in adjusting and maintaining homeostatic balance within the infant intestine. The aim of this study was to elucidate the relationship between maternal and infant gut microbiota and identify the Bifidobacterium species that may transfer from mother to infant over the first 42 days of the infant's life. Nineteen mother-infant-pair fecal samples were collected and the diversity and composition of the total bacterial and Bifidobacterium communities were analyzed via 16S rDNA and bifidobacterial groEL gene high throughput sequencing. The results revealed that the relative abundance of Bifidobacterium was significantly higher in the infant gut while Parabacteroides, Blautia, Coprococcus, Lachnospira and Faecalibacterium were at lower relative abundance in 7-day and 42-day infant fecal samples compared to the maternal samples. The maternal gut has more B. pseudocatenulatum. In the infant group, B. breve and B. dentium relative abundance increased while B. animalis subsp. lactis decreased from days 7 to 42. Additionally, B. longum subsp. longum isolated from FGZ16 and FGZ35 may have transferred from mother to infant and colonized the infant gut. The results of the current study provide insight toward the infant gut microbiota composition and structure during the first 42 days and may help guide Bifidobacterium supplementation strategies in mothers and infants.

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“…Further CDSs found in the genome of 1654_15 potentially related to virulence and pathogenicity of Mycoplasma sp. 1654_15 were, for example, a glycerol-3-phosphate oxidase (glpO, locus tag HF996_02520) shown to be involved in the production of cytotoxic H 2 O 2 in other mycoplasmas [33,34], a predicted sialic acid scavenging and degradation pathway comprising two exo-alpha-sialidases (HF996_00850 and _00865), a N-acetylmannosamine-6-phsophate 2-isomerase (nanE, HF996_00590), a sialic acid transporter (HF996_00595), a Nacetylneuraminate lyase (nanA, HF996_00605), and a N-acetylmannosamine kinase (nanK, locus tag HF996_00610), a predicted immunoglobuline A1 proteases (HF996-00875), a glycosyl transferase (HF996_02470) possibly contributing to capsule formation [35], as well as two tandem pairs of Mycoplasma Ig binding (MIB, HF996_03795 and _03750)/Mycoplasma Ig protease (MIP, HF996_03790 and _03745) proteins in contrast to one pair found in M. hyorhinis [36]. To analyse strain 1654_15 at the protein level, a proteome-wide comparison using average amino acid identity (AAI) profiler and Proteome Comparison tool at PATRIC was performed.…”

Section: Characterization Of Mycoplasma Sp Isolates Cultivated From mentioning

confidence: 99%

Swine Conjunctivitis Associated with a Novel Mycoplasma Species Closely Related to Mycoplasma hyorhinis

Hennig-Pauka¹,

Sudendey²,

Kleinschmidt

et al. 2020

Pathogens

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Conjunctivitis in swine is a common finding, usually considered to be a secondary symptom of respiratory or viral systemic disease, or a result of irritation by dust or ammonia, or of local infections with Mycoplasma (M.) hyorhinis or chlamydia. In three unrelated swine farms in Germany with a high prevalence of conjunctivitis, a novel mycoplasma species, tentatively named Mycoplasma sp. 1654_15, was isolated from conjunctival swabs taken from affected pigs. Although 16S rRNA gene sequences shared highest nucleotide similarities with M. hyorhinis, matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry, partial rpoB sequencing, and comparative whole genome analyses indicated the identification of a novel species within genus Mycoplasma. Noticeable differences between Mycoplasma sp. 1654_15 and M. hyorhinis were the lack of a vlp locus and the presence of a myo-inositol pathway in the genome of strain 1654_15. Since myo-inositol might be used as an alternative energy source by this pathogen on the conjunctival surface, robust colonization by outcompeting other bacteria could be the consequence. In summary, abundant isolation of Mycoplasma sp. 1654_15 from the conjunctiva of affected pigs, its close relationship to M. hyorhinis, and identification of a panel of coding sequences (CDSs) potentially associated with virulence and pathogenicity suggested a local eye disease caused by a so far unknown, highly specialized mycoplasma species.

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Tn-sequencing of Mycoplasma hyopneumoniae and Mycoplasma hyorhinis mutant libraries reveals non-essential genes of porcine mycoplasmas differing in pathogenicity

Cited by 13 publications

References 28 publications

DnaK Functions as a Moonlighting Protein on the Surface of Mycoplasma hyorhinis Cells

DnaK Functions as a Moonlighting Protein on the Surface of Mycoplasma hyorhinis Cells

Development of gut microbiota and bifidobacterial communities of neonates in the first 6 weeks and their inheritance from mother

Swine Conjunctivitis Associated with a Novel Mycoplasma Species Closely Related to Mycoplasma hyorhinis

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