TNFα Triggers an Augmented Inflammatory Response in Brain Neurons from Dahl Salt-Sensitive Rats Compared with Normal Sprague Dawley Rats

Gao, Huanjia; Bigalke, Jeremy; Fan, Yuanyuan; Chen, Bojun; Chen, Qing‐Hui; Shan, Zhiying

doi:10.1007/s10571-021-01056-9

Cited by 11 publications

(10 citation statements)

References 43 publications

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“…In neuroscience research, primary neuron cells derived from postnatal rat brains are extensively used as an in vitro model to explore neuronal development, function, and neurological disorders. 22,25 Neurons were isolated from the brains of 1-day-old SD pups using a papain dissociation system (Worthington Biochemical Co.) following the manufacturer's instruction. First, SD pups were euthanized using an overdose of isoflurane, and their whole brains were removed and placed in a sterile culture dish containing cold PBS.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

Section: Animalsmentioning

confidence: 99%

“…This study was carried out in strict accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health. The Michigan Technological University Institutional Animal Care and Use Committee approved the protocol 22.…”

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confidence: 99%

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Engineering Exosomes to Specifically Target the Mitochondria of Brain Cells

Yan,

Chen,

Shan

et al. 2023

ACS Omega

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Mitochondrial dysfunction is associated with various health conditions, including cardiovascular and neurodegenerative diseases. Mitochondrial-targeting therapy aims to restore or enhance mitochondrial function to treat or alleviate these conditions. Exosomes, small vesicles that cells secrete, containing a variety of biomolecules, are critical in cell-to-cell communication and have been studied as potential therapeutic agents. Exosome-based therapy has the potential to treat both cardiovascular and neurodegenerative diseases. Combining these two approaches involves using exosomes as carriers to transport mitochondrial-targeting agents to dysfunctional or damaged mitochondria within target cells. This article presents a new technique for engineering brain-derived exosomes that target mitochondria and has demonstrated promise in initial tests with primary neuron cells and healthy rats. This promising development represents a significant step forward in treating these debilitating conditions.

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Section: ■ Materials and Methodsmentioning

confidence: 99%

Section: Animalsmentioning

confidence: 99%

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Engineering Exosomes to Specifically Target the Mitochondria of Brain Cells

Yan,

Chen,

Shan

et al. 2023

ACS Omega

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“… 42 , 43 TNF-α is one of the important inflammatory indicators of hypertensive plaques, which can inhibit autophagy and aggravate the formation of hypertensive plaques by inducing p38MAPK phosphorylation. 44 , 45 Reducing serum IL-1β and nuclear factor kappa B (NF-κB) levels can improve hypothalamic leptin resistance, thereby inhibiting central sympathetic nerve excitation-mediated increase in blood pressure. 46 , 47 The above cytokines also mediate the inflammatory response of acne.…”

Section: The Relationship Between Acne and Various Metabolic Diseasesmentioning

confidence: 99%

Acne Comorbidities

Wang¹,

Zhu²,

Wu³

et al. 2022

CCID

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Acne vulgaris is a multifactorial chronic inflammatory disorder of the pilosebaceous unit, and it represents the most common skin disease affecting about 85% of adolescents in Western populations. The prevalence of acne vulgaris in developed countries is higher than that in developing countries.Emerging data has shown some systemic diseases closely associated with acne, including obesity, diabetes mellitus, cardiovascular diseases, metabolic syndrome (Mets), and so on.This review summarizes acneassociated diseases that have been reported in studies, and analyzes the possible co-pathogenesis of these diseases and acne.

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“…At the biomolecular level, caspase-1 and tumor necrosis factor-alpha (TNF-alpha) are two key proteins involved in the development and progression of dysregulated inflammation. From one side, caspase-1 acts as an inflammatory response initiator, inducing a proinflammatory response by cleaving (and thus activating) two inflammatory cytokines [ 6 , 7 , 8 ] known as interleukin 1β (IL-1β) and interleukin 18 (IL-18) while inducing pyroptosis (a programmed lytic cell-death pathway) [ 9 , 10 ] through the cleavage of the protein gasdermin D. On the other hand, TNF-alpha is a cytokine that has been identified as a central player in the pathogenesis of inflammation and autoimmune diseases [ 11 ] and can trigger several inflammation-related proteins such as caspase-1 [ 12 ], as well as other cytokines and chemokines [ 13 ]. An important aspect to highlight here is that both caspase-1 and TNF-alpha are essential in the development of the hyperinflammatory physiological reaction known as cytokine storm, which is present in viral infections caused by coronaviruses [ 14 , 15 ], including the causal agent of the current COVID-19 pandemic (SARS-CoV-2) [ 14 , 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

In Silico Drug Repurposing for Anti-Inflammatory Therapy: Virtual Search for Dual Inhibitors of Caspase-1 and TNF-Alpha

2021

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Inflammation involves a complex biological response of the body tissues to damaging stimuli. When dysregulated, inflammation led by biomolecular mediators such as caspase-1 and tumor necrosis factor-alpha (TNF-alpha) can play a detrimental role in the progression of different medical conditions such as cancer, neurological disorders, autoimmune diseases, and cytokine storms caused by viral infections such as COVID-19. Computational approaches can accelerate the search for dual-target drugs able to simultaneously inhibit the aforementioned proteins, enabling the discovery of wide-spectrum anti-inflammatory agents. This work reports the first multicondition model based on quantitative structure–activity relationships and a multilayer perceptron neural network (mtc-QSAR-MLP) for the virtual screening of agency-regulated chemicals as versatile anti-inflammatory therapeutics. The mtc-QSAR-MLP model displayed accuracy higher than 88%, and was interpreted from a physicochemical and structural point of view. When using the mtc-QSAR-MLP model as a virtual screening tool, we could identify several agency-regulated chemicals as dual inhibitors of caspase-1 and TNF-alpha, and the experimental information later retrieved from the scientific literature converged with our computational results. This study supports the capabilities of our mtc-QSAR-MLP model in anti-inflammatory therapy with direct applications to current health issues such as the COVID-19 pandemic.

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TNFα Triggers an Augmented Inflammatory Response in Brain Neurons from Dahl Salt-Sensitive Rats Compared with Normal Sprague Dawley Rats

Cited by 11 publications

References 43 publications

Engineering Exosomes to Specifically Target the Mitochondria of Brain Cells

Engineering Exosomes to Specifically Target the Mitochondria of Brain Cells

Acne Comorbidities

In Silico Drug Repurposing for Anti-Inflammatory Therapy: Virtual Search for Dual Inhibitors of Caspase-1 and TNF-Alpha

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