Tobacco mosaic virus adsorption on self-assembled and Langmuir–Blodgett monolayers studied by TIRF and SFM

Britt, David W.; Buijs, Jos; Hlady, Vladimir

doi:10.1016/s0040-6090(98)00770-6

Cited by 26 publications

(19 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This phenomenon is probably connected with hydrophobic interaction of the bacteriophage protein head with graphite surface, which leads to the partial destruction of a phage particle. HOPG-induced partial destruction of tobacco mosaic virus was also reported elsewhere [25]–[26]. The shapes of the height histograms presented in figures 3C, F have two maximums, which demonstrate the presence of two states of bacteriophages: with full and (partially) empty heads.…”

Section: Resultssupporting

confidence: 78%

Atomic Force Microscopy Analysis of the Acinetobacter baumannii Bacteriophage AP22 Lytic Cycle

et al. 2012

View full text Add to dashboard Cite

Background Acinetobacter baumannii is known for its ability to develop resistance to the major groups of antibiotics, form biofilms, and survive for long periods in hospital environments. The prevalence of infections caused by multidrug-resistant A. baumannii is a significant problem for the modern health care system, and application of lytic bacteriophages for controlling this pathogen may become a solution.Methodology/Principal FindingsIn this study, using atomic force microscopy (AFM) and microbiological assessment we have investigated A. baumannii bacteriophage AP22, which has been recently described. AFM has revealed the morphology of bacteriophage AP22, adsorbed on the surfaces of mica, graphite and host bacterial cells. Besides, morphological changes of bacteriophage AP22-infected A. baumannii cells were characterized at different stages of the lytic cycle, from phage adsorption to the cell lysis. The phage latent period, estimated from AFM was in good agreement with that obtained by microbiological methods (40 min). Bacteriophage AP22, whose head diameter is 62±1 nm and tail length is 88±9 nm, was shown to disperse A. baumannii aggregates and adsorb to the bacterial surface right from the first minute of their mutual incubation at 37°C.Conclusions/SignificanceHigh rate of bacteriophage AP22 specific adsorption and its ability to disperse bacterial aggregates make this phage very promising for biomedical antimicrobial applications. Complementing microbiological results with AFM data, we demonstrate an effective approach, which allows not only comparing independently obtained characteristics of the lytic cycle but also visualizing the infection process.

show abstract

Section: Resultssupporting

confidence: 78%

Atomic Force Microscopy Analysis of the Acinetobacter baumannii Bacteriophage AP22 Lytic Cycle

et al. 2012

View full text Add to dashboard Cite

show abstract

“…glass slides with positive surface charge, a self assembled monolayer of EDA (N-(2-aminoethyl)(3-aminopropyl) trimethoxysilane) was established following the method described byStenger et al (1992) andBritt et al (1998), except that the application of EDA was conducted in a He atmosphere. The resulting zeta potential of glass beads following the same procedure was approximated by Smoluchowsky's equation after measuring the electrophoretic mobility with a Zetamaster (Malvern Instruments, USA).…”

mentioning

confidence: 99%

Virus removal by unsaturated wastewater filtration: effects of biofilm accumulation and hydrophobicity

Heistad

Scott

Skaarer

et al. 2009

Water Science and Technology

View full text Add to dashboard Cite

Enhanced treatment of septic tank effluent can improve the hydraulic function and performance of infiltration systems and constructed wetlands. By intermittent spray application of septic tank effluent onto a coarse-grained filter media, an unsaturated flow regime beneficial for pathogen removal is created. A column filtration study showed an increase in PRD-1 removal by time of operation with corresponding biofilm accumulation in the filter material. The same increased removal was observed for 1 mum polystyrene beads, irrespective of their hydrophilic/hydrophobic surface properties. A control experiment with sorption of 1 mum hydrophobic and hydrophilic polystyrene beads to different glass surfaces with hydrophobic and hydrophilic properties indicate that mechanisms other than hydrophobic interactions may govern the rate of attachment to the filter media. For a given volumetric flow-rate in the columns, the presence of biofilm altered the hydrodynamic characteristics and this resulted in increased retention time and particle removal.

show abstract

“…This means that these viruses have been partially split upon adsorption. This fact has been already observed on hydrophobic surfaces (Britt et al, 1998) and later on graphene (Dubrovin et al, 2004). Britt et al carried out a kinetic study of TMV adsorption on organic monolayers, observing that, while both electrostatic and hydrophobic interactions influence TMV adsorption, only in the case of hydrophobic surfaces viral disassembly and shortening occurs.…”

Section: Discussionmentioning

confidence: 65%

Key factors of scanning a plant virus with AFM in air and aqueous solution

Eleta-Lopez

Calò

2016

Microscopy Res & Technique

View full text Add to dashboard Cite

For tobacco mosaic virus (TMV) as a model virus, this article shows typical issues of scanning soft biological matter by atomic force microscopy (AFM). TMV adsorbed on chemically different flat surfaces, gold, mica, and APDMES-functionalized silicon, is studied in air and aqueous environment. In air, the TMV particles arrangement shows some variety, depending on the substrate. The height of TMV is reduced to 13.7, 15.8, and 15.6 nm, for gold, APDMES, and mica, respectively while the width is about ∼30 nm due to the influence of the tip radius. In aqueous solution, the surface charges of the virus and the solid support play an important role in the virus adsorption process. While deposition on negatively charged mica is favored only at low pH values, it is shown that positively charged APDMES functionalized silicon can be a suitable substrate to work with at neutral pHs. The effects of cantilever oscillation's free amplitude (A ) and the amplitude set-point (A) are also assessed here. While high A prompt reversible deformation of TMV in measurements performed in air, irreversible damage of the virus in liquid conditions (water) is observed using stiff cantilevers (0.35 N m ) and high A (81 nm), leading to a 6 nm reduction in the height of TMV after the first scan. Finally, low values of the amplitude set-point (A/A = 0.3), which means applying higher forces to the sample, also brings the damage of TMV virus assemblies, reducing its monolayer roughness to 0.3 nm. Microsc. Res. Tech. 80:18-29, 2017. © 2016 Wiley Periodicals, Inc.

show abstract

Tobacco mosaic virus adsorption on self-assembled and Langmuir–Blodgett monolayers studied by TIRF and SFM

Cited by 26 publications

References 17 publications

Atomic Force Microscopy Analysis of the Acinetobacter baumannii Bacteriophage AP22 Lytic Cycle

Atomic Force Microscopy Analysis of the Acinetobacter baumannii Bacteriophage AP22 Lytic Cycle

Virus removal by unsaturated wastewater filtration: effects of biofilm accumulation and hydrophobicity

Key factors of scanning a plant virus with AFM in air and aqueous solution

Contact Info

Product

Resources

About