Tocopherols and Tocotrienols: an Adapted Methodology by UHPLC/MS Without Sample Pretreatment Steps

Quality Parameters of Mechanically Extracted Edible Macauba Oils (Acrocomia aculeata) for Potential Food and Alternative Industrial Feedstock Application

“…Oil stability index: AOCS Official Method Cd 12b‐92 Tocols content: Determined by Ultra High‐Performance Liquid Chromatography coupled to Mass Spectrometry (UHPLC/MS), according to an adapted methodology previously optimized by Ansolin et al …”

“…[34] Oil stability index: AOCS Official Method Cd 12b-92. [34] Tocols content: Determined by Ultra High-Performance Liquid Chromatography coupled to Mass Spectrometry (UHPLC/MS), according to an adapted methodology previously optimized by Ansolin et al [37] The analytical results were performed by analysis of variance. Differences were tested between means at 5% probability [38] by Tukey's test.…”

Effect of the type and level of hydration of alcoholic solvents on the simultaneous extraction of oil and chlorogenic acids from sunflower seed press cake

“…The determination of tocopherol content in SFO obtained by sequential extraction in three stages of contact at 90 °C, with alcoholic solvents, as well as in crude and refined SFO, was accomplished using ultra‐performance liquid chromatography coupled with mass spectrometry (Acquity Ultra performance; Waters, Milford, MA, USA) and a single quadrupole detector (single quadrupole) (Waters). Data were analyzed using Mass Lynx, version 4.1 (Waters) in accordance with the methodology suggested by Ansolin et al …”

“…The determination of tocopherol content in SFO obtained by sequential extraction in three stages of contact at 90 ∘ C, with alcoholic solvents, as well as in crude and refined SFO, was accomplished using ultra-performance liquid chromatography coupled with mass spectrometry (Acquity Ultra performance; Waters, Milford, MA, USA) and a single quadrupole detector (single quadrupole) (Waters). Data were analyzed using Mass Lynx, version 4.1 (Waters) in accordance with the methodology suggested by Ansolin et al 29 The analysis of phosphorus content was carried out by plasma coupled optical emission spectrometry in accordance with method Ca 20-99. 26 According to the method Ca 12-55 26 , the amount of phosphorus was converted into equivalent phospholipids, multiplying the result in terms of phosphorus by the conversion factor 30.…”

Determination of tocopherols and tocotrienols in human breast adipose tissue with the use of high performance liquid chromatography–fluorescence detection

“…Qualitative and quantitative determination of T and T3 in various matrices is usually performed with the application of fluorescence (FLD) (Chajès, Lhuillery, Sattler, Kostner, & Bougnoux, ; Handelman, Epstein, Machlin, van Kuijk, & Dratz, ; Rupérez, Barbas, Castro, Martínez, & Herrera, ; Shim et al, ; Takahashi et al, ; Weiser, Riss, & Kormann, ; Yeum et al, ) and diode‐array detection (Gleize, Steib, André, & Reboul, ; Yang et al, ; Yuan, Ju, Jin, Ren, & Liu, ). Mass spectrometry (MS) is rarely implemented in determination of T and T3 (Ansolin, de Souza, de Almeida Meirelles, & Batista, ; Bartosińska, Buszewska‐Forajta, & Siluk, ; Nagy et al, ; Viñas et al, ) as the ionization process of these compounds is problematic. To our best knowledge, few authors have described the use of LC–MS methods for the simultaneous quantification of more than one tocochromanol, for instance the normal‐phase liquid chromatography coupled with atmospheric pressure chemical ionization mass spectrometry (NP–LC–APCI–MS) method presented by Nagy et al (), the RP–LC–APCI–MS method described by Viñas et al (), the reversed‐phase liquid chromatography coupled with electrospray ionization mass spectrometry (RP–LC–ESI–MS) method by Bustamante‐Rangel, Delgado‐Zamarreño, Sánchez‐Pérez, & Carabias‐Martínez () or the RP–UPLC–ESI–MS n method presented by Górnaś, Pugajeva, & Segliņa ().…”