Tolerance of blackgrass (Alopecurus myosuroide) to sylfonylurea herbicides in the Czech Republic

Abstract

Slavíková L, Mikulka J., Kundu J.K. (2011): Tolerance of blackgrass (Alopecurus myosuroide) to sylfonylurea herbicides in the Czech Republic. Plant Protect. Sci., 47: 55-61.Seeds of three blackgrass populations from Southern Bohemia were collected in [2007][2008]. Biological tests with chlorsulfuron were performed at the 2-3 leaf stage. Some plants survived after treatment with the highest dose 37.5 g/ha. Biological tests showed a resistant phenotype to chlorsulfuron. Leaves of these plants were analysed by dCAPS assay. Two domains of ALS gene: domain A -P197 and domain B -W574 were targeted by PCR with regenerated primers P197 containing BamHI site and W574 containing site BstXI. PCR products of all tested samples were cleaved by BamHI in the codon P197. No mutation of proline in P197 was found out. The codon W574 PCR product of the samples was not cleaved by BstXI.

Weed Resistance to Herbicides in the Czech Republic: History, Occurrence, Detection and Management

“…Alopecurus myosuroides is the weed with local importance in the Czech Republic. However, the poor weed control had been reported in several fields and the tested populations showed sulfonylurea resistant phenotype (Slavíková et al, 2011). On arable land, the most important seems to be ALS-resistant Apera spica-venti (Hamouzová et al, 2011).…”