Tolerance to repeated stress in rats with lesions of the serotoninergic neurons of the Median Raphe Nucleus and chronically treated with imipramine

Silva, Kely Rodrigues da; Carvalho, Milene Cristina de; Padovan, Cláudia Maria

doi:10.1016/j.bbr.2016.01.025

Cited by 6 publications

(1 citation statement)

References 27 publications

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“…Rats were divided into three groups. Group 1 acted as control and received only the vehicle (DMSO: Tween: distilled water (1:1:8)) and group 2 (positive control) received a single-dose of imipramine hydrochloride ® (15 mg/kg) dissolved in the vehicle (Silva et al 2016). Group 3 received the alkaloidal fraction (100 mg/kg dissolved in the vehicle).…”

Section: Forced Swimming Test (Fst)mentioning

confidence: 99%

Antidepressant potential of Mesembryanthemum cordifolium roots assisted by metabolomic analysis and virtual screening

Said

Ali

Attia

et al. 2020

Natural Product Research

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Depression is a common mental disturbance that can be categorized as mild, moderate or severe. Mesemberine alkaloids, the main recognized phytoconstituents of some plants belonging to family Mesembryanthemaceae, are well-known as serotonin reuptake inhibitors. Therefore, the objective of this study is to evaluate the antidepressant activity of the alkaloidal fraction of Mesembryanthemum cordifolium L.f. (Aptenia cordifolia) roots, family Mesembryanthemaceae using forced swimming test, assisted by metabolomic analysis and in silico ligand-based and structure-based screening. Results showed that the alkaloidal fraction displayed an antidepressant activity superior to imipramine hydrochloride, a standard antidepressant agent. Nine alkaloids were annotated from the metabolomic analysis. Interestingly, among the dereplicated constituents, mesembrane (5) displayed strong binding affinity to SERT protein, which is slightly higher than the antidepressant drug venlafaxine. In conclusion, the alkaloidal fraction of the M. cordifolium (A. cordifolia) root exhibits an antidepressant activity which can be attributed in part to mesembrane (5).

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Section: Forced Swimming Test (Fst)mentioning

confidence: 99%

Antidepressant potential of Mesembryanthemum cordifolium roots assisted by metabolomic analysis and virtual screening

Said

Ali

Attia

et al. 2020

Natural Product Research

View full text Add to dashboard Cite

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Behavioral Consequences of Hippocampal 5‐HT7 Receptors Blockade in Stressed Rats

Pereira,

Gonçalez,

Prado

et al. 2024

Hippocampus

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Serotonin (5‐HT) has long been involved in response to stress and its effect may be, in part, mediated by 5‐HT1a and 5‐HT7 receptor subtypes in different brain structures. Both pre‐ and post‐synaptic activation of 5‐HT1a receptor, respectively, in the rat median raphe nucleus (MnRN) and hippocampus, lead to adaptation to acute inescapable stressors such as restraint and forced swim. 5‐HT7 receptor (5HT7r), a stimulatory G‐protein coupled receptor, has also been investigated as a possible candidate for mediating stress response. In the MnRN, activation of 5‐HT7r has antidepressant effects, while in the hippocampus, 5HT7r mRNA expression is increased after exposure to restraint stress, but the functional significance of these receptors remains to be determined. Therefore, the aim of this study was to investigate whether blockade of hippocampal 5HT7r would prevent and/or attenuate the behavioral effects of stress. Male adult Wistar rats with bilateral cannulas aimed at the dorsal hippocampus were restrained for 2 h and tested in the elevated plus maze (EPM) 24 h later. SB 258741 (3 nmoles/0.5 μL/side; selective 5HT7r antagonist) was administered bilateraly into the hippocampus according to the experimental protocol: immediately before or after stress, or 24 h after it (immediately before the test). In a second experiment, rats were exposed to 15 min. of forced swim, and tested 24 h later. Intra‐hippocampal treatment was performed as described for the restraint stress protocol. We found that blockade of hippocampal 5‐HT7r immediately after, but not before, the exposure to restraint or forced swim attenuated stress‐induced behavioral changes. Similar results were obtained when SB was administered before the test in previously stressed rats. Our data suggest that activation of hippocampal 5‐HT7r is crucial for the consolidation and retrieval of aversive stimulus‐related memories, such as those caused by a stressful experience, probably through mechanisms involving stress‐induced changes in 5‐HT7r expression.

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Both serotonergic and noradrenergic systems modulate the development of tolerance to chronic stress in rats with lesions of the serotonergic neurons of the median raphe nucleus

Pereira

Carvalho

Padovan

2019

Behavioural Brain Research

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Tolerance to repeated stress in rats with lesions of the serotoninergic neurons of the Median Raphe Nucleus and chronically treated with imipramine

Cited by 6 publications

References 27 publications

Antidepressant potential of Mesembryanthemum cordifolium roots assisted by metabolomic analysis and virtual screening

Antidepressant potential of Mesembryanthemum cordifolium roots assisted by metabolomic analysis and virtual screening

Behavioral Consequences of Hippocampal 5‐HT7 Receptors Blockade in Stressed Rats

Both serotonergic and noradrenergic systems modulate the development of tolerance to chronic stress in rats with lesions of the serotonergic neurons of the median raphe nucleus

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