Toll-like Receptor 4 Signaling Confers Cardiac Protection against Ischemic Injury via  Inducible Nitric Oxide Synthase- and Soluble Guanylate Cyclase-dependent Mechanisms

Abstract

Background: Prior administration of a small dose of lipopolysaccharide confers a cardiac protection against ischemiareperfusion injury. However, the signaling mechanisms that control the protection are incompletely understood. We tested the hypothesis that Toll-like receptor 4 (TLR4) mediates the ability of lipopolysaccharide to protect against cardiac ischemia-reperfusion injury through distinct intracellular pathways involving myeloid differentiation factor 88 (MyD88), TIR-domain-containing adaptor protein-inducing interferon-␤-mediated transcription factor (Trif), in-

Toll-like Receptors in the Vascular System: Sensing the Dangers Within

“…LPS-induced TLR4 activation before myocardial ischemia has been also shown to have cardioprotective effects (Zacharowski et al, 2000). These effects have been primarily attributed to a TLR4-mediated increased production in inducible nitric oxide synthase and nitric 156 oxide with improvements in ventricular function (Wang et al, 2011a).…”

Myocardial Ischemia Activates an Injurious Innate Immune Signaling via Cardiac Heat Shock Protein 60 and Toll-like Receptor 4

“…Ex Vivo Model of Myocardial I/R-The ex vivo model has been described previously (20,24). Briefly, mice were heparinized and euthanized.…”

High Mobility Group Box 1 Inhibits Human Pulmonary Artery Endothelial Cell Migration via a Toll-like Receptor 4- and Interferon Response Factor 3-dependent Mechanism(s)

“…As a final method to examine MyD88-dependent versus MyD88-independent signaling in HPAECs we examined downstream induction of MyD88/NFkB-dependent (iNOS) (27,28) and IRF3-dependent (IFN␤ and ISG20) (29,30) genes by RT-PCR. As expected, HMGB1 stimulated the induction of IFN␤ and ISG20 but not iNOS in HPAECs (Fig.…”