Toll-like receptor agonists stimulate differential functional activation and cytokine and chemokine gene expression in heterophils isolated from chickens with differential innate responses

Kogut, Michael H.; Swaggerty, Christina L.; He, Haiqi; Pevzner, I. Y.; Kaiser, Pete

doi:10.1016/j.micinf.2006.02.026

Cited by 101 publications

(73 citation statements)

References 29 publications

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“…Cytokines, chemokines and nitric oxide are induced via chTLRs in response to their agonists (20,30,31). These findings imply that chTLR2 (30), chTLR15 (19), chTLR4 (20), chTLR5 (32), chTLR7 (33) and chTLR3 (34) are involved in leukocyte activation in chicken.…”

mentioning

confidence: 81%

Combinational recognition of bacterial lipoproteins and peptidoglycan by chicken Toll-like receptor 2 subfamily

Higuchi

Matsuo

Shingai

et al. 2008

Developmental & Comparative Immunology

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2 SummaryHuman TLR2 subfamily recognizes bacterial lipoproteins (BLP) and peptidoglycan (PGN). According to the genome information, chicken has structural orthologs of TLRs 1 and 2, in addition to TLRs3, 4, 5 and 7. Chicken has two additional TLRs, TLR15 and TLR21, which orthologs human lacks. The chicken (ch)TLR1 and 2 genes are individually duplicated to encode for two different proteins, chTLRs, chTLR1-1, 1-2, 2-1 and 2-2, of the TLR2 subfamily. Here we investigated the functional profile of these TLR2 subfamily proteins of chicken. By NF-κB reporter assay using HEK293 cells, we found that chTLR2-1 and chTLR1-2 cooperatively signal the presence of PGN. A combination of chTLR2-1 and chTLR1-2 also most efficiently recognized diacylated BLP, Malp-2, while the combination of chTLR2-1 and chTLR1-1 failed to recognize Malp-2. All combinations, however, recognized triacylated BLP, Pam3. Consistent with these results, human TLR2-stimulating mycobacteria preparations, BCG-cell wall and cell lysate of Mycobacterium avium, induced activation of NF-κB in cells expressing chTLR2-1 and 1-2 and to lesser extents, cells with chTLR2-2 and either of chTLR1. Strikingly, expression of either of these alone did not activate the reporter for NF-κB. These chTLRs are likely to have the combination functional feature as in the human TLR2 subfamily. Confocal and immunoprecipitation analyses of human cell transfectants showed that they cluster on the cell surface by a physical molecular association, causing all of them to merge and coprecipitate. These results suggest that chTLR2 subfamily members discriminate between their ligands by combinational events.

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mentioning

confidence: 81%

Combinational recognition of bacterial lipoproteins and peptidoglycan by chicken Toll-like receptor 2 subfamily

Higuchi

Matsuo

Shingai

et al. 2008

Developmental & Comparative Immunology

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“…Binding of PAMPs leads to TLR activation, which, in turn, initiates MAPKor nuclear factor kappa B (NFkB)-dependent cascades that culminate in a proinflammatory response. This response involves the secretion of cytokines and antibacterial substances, such as b-defensins (Froy 2005, Kogut et al 2006. TLRs and b-defensins have been identified as the key components of innate immune recognition in the reproductive tract of male vertebrate species, providing the first line of defense against potential pathogens (Li et al 2001, Com et al 2003, Palladino et al 2003, Avellar et al 2004, Nishimura & Naito 2005, Rodrigues et al 2008, Anastasiadou et al 2011.…”

Section: Introductionmentioning

confidence: 99%

Activation of innate immune system in response to lipopolysaccharide in chicken Sertoli cells

Michailidis¹,

Anastasiadou²,

Guibert³

et al. 2014

Reproduction

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Sertoli cells (SCs) play an important physiological role in the testis, as they support, nourish, and protect the germ cells. As protection of the developing spermatozoa is an emerging aspect of reproductive physiology, this study examined the expression pattern of innate immune-related genes, including avian b-defensins (AvBDs), Toll-like receptors (TLRs), and cytokines, and investigated the time course of an inflammatory response in rooster SCs triggered by exposure to the bacterial endotoxin lipopolysaccharide (LPS). SCs were isolated from 6-week-old chicken, cultured in vitro, and stimulated with 1 mg/ml LPS at different time courses (0, 6, 12, 24, and 48 h). Data on expression analysis revealed that all ten members of the chicken TLR family, nine members of the AvBD family, as well as eight cytokine genes were expressed in SCs. Quantitative real-time PCR analysis revealed that LPS treatment resulted in significant induction of the expression levels of six TLRs, six AvBDs, and four cytokine genes, while two cytokine genes were downregulated and two other genes were unchanged. The increasing interleukin 1b (IL1b) production was confirmed in the conditioned medium. Furthermore, the phagocytosis of SCs was increased after LPS treatment. In conclusion, these findings provide evidence that SCs express innate immunerelated genes and respond directly to bacterial ligands. These genes represent an important component of the immune system, which could be integrated into semen, and present a distinctive constituent of the protective repertoire of the testis against ascending infections.

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“…Both T cell subsets were recruited in the efferent duct and ductus deferens, and likewise with CD8+ T cells in the rete testis. LPS can be recognized by TLR4 (Temperley et al, 2008), and the Zhang et al: T cells in Seminal Tract interaction of LPS with TLR4 can lead to the expression of proinflammatory cytokines and chemokines (Kogut et al, 2006). IL-1β may induce T cell proliferation (Staeheli et al, 2001), whereas CXCLi2 may be involved in the recruitment of heterophils (Poh et al, 2008;Redmond et al, 2011), monocytes (Barker et al, 1993), and T cells (Min et al, 2002) to the site of infection in birds.…”

Section: Discussionmentioning

confidence: 99%

“…Lipopolysaccharide (LPS), a pathogenic component of Gram-negative bacteria, induces pathophysiological responses (Tilders et al, 1994). It activates some types of host immune cells via Tolllike receptor (TLR) 4 signaling pathways (Muzio et al, 2000;Temperley et al, 2008), leading to upregulation of proinflammatory cytokine and chemokine expression (Kogut et al, 2006). The T cell subsets play an essential role in cellmediated immune response in order to eliminate pathogenic organisms.…”

Section: Introductionmentioning

confidence: 99%

Effects of Lipopolysaccharide on the Recruitment of T Cells in the Seminal Tract of Roosters

2013

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Toll-like receptor agonists stimulate differential functional activation and cytokine and chemokine gene expression in heterophils isolated from chickens with differential innate responses

Cited by 101 publications

References 29 publications

Combinational recognition of bacterial lipoproteins and peptidoglycan by chicken Toll-like receptor 2 subfamily

Combinational recognition of bacterial lipoproteins and peptidoglycan by chicken Toll-like receptor 2 subfamily

Activation of innate immune system in response to lipopolysaccharide in chicken Sertoli cells

Effects of Lipopolysaccharide on the Recruitment of T Cells in the Seminal Tract of Roosters

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