Tomato Yellow Leaf Curl Virus, a Whitefly-Borne Geminivirus of Tomatoes

Cohen, S.; Antignus, Y.

doi:10.1007/978-1-4612-2590-4_10

Cited by 148 publications

(89 citation statements)

References 66 publications

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“…TYLCV-infected tomatoes are distinguished by curling of their leaves (36), and tomato plants carrying a mutation within the SGS3 locus exhibit an aberrant leaf phenotype (Y. Eshed, personal communication). Consistent with this effect on leaf morphology, SGS3 also has been implicated in determining the adaxial identity of the leaf (37).…”

Section: Discussionmentioning

confidence: 99%

Interaction with host SGS3 is required for suppression of RNA silencing by tomato yellow leaf curl virus V2 protein

Glick

Zrachya

Levy

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

276

202

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The V2 protein of tomato yellow leaf curl geminivirus (TYLCV) functions as an RNA-silencing suppressor that counteracts the innate immune response of the host plant. The host-cell target of V2, however, remains unknown. Here we show that V2 interacts directly with SlSGS3, the tomato homolog of the Arabidopsis SGS3 protein (AtSGS3), which is known to be involved in the RNA-silencing pathway. SlSGS3 genetically complemented an AtSGS3 mutation and restored RNA silencing, indicating that SlSGS3 is indeed a functional homolog of AtSGS3. A point mutant of V2 that is unable to bind SlSGS3 also lost its ability to suppress RNA silencing, suggesting a correlation between the V2–SlSGS3 interaction in planta and the suppressor activity of V2.

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Section: Discussionmentioning

confidence: 99%

Interaction with host SGS3 is required for suppression of RNA silencing by tomato yellow leaf curl virus V2 protein

Glick

Zrachya

Levy

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

276

202

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“…Tomato yellow leaf curl virus (TYLCV), a major pathogen of tomato crops in the Mediterranean basin, southern Asia, Africa and Central America, is a geminivirus transmitted by the whitefly Bemisia tabaci (Cohen & Antignus, 1994). In contrast to most other known whitefly-transmitted geminiviruses, whose genomes are split between two single-stranded (ss) DNA components (DNA A and DNA B), the TYLCV genome consists of a single circular ssDNA molecule of 2787 nucleotides (Kheyr-Pour et al, 1991 ;Navot et al, 1991 ;Noris et al, 1994).…”

Section: Introductionmentioning

confidence: 99%

The capsid protein of tomato yellow leaf curl virus binds cooperatively to single-stranded DNA.

Palanichelvam

Kunik

Citovsky

et al. 1998

Journal of General Virology

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“…Older whiteflies still transmit but at a lower frequency. Multiple whiteflies per test plant (15-40 per plant) should be used for high rates of transmission since a ratio of 1 whitefly per plant often results in unacceptably low transmission rates 3,4 . The number needed depend upon the virus and the species of the acquisition and test plants.…”

Section: Methods For Inoculation Of Test Plantsmentioning

confidence: 99%

“…Many of these viruses are unable or cannot be easily transmitted by other means. Therefore maintenance of virus cultures, biological and molecular characterization (identification of host range and symptoms) 3,13 , ecology 2,12 , require that the viruses be transmitted to experimental hosts using the whitefly vector. In addition the development of new approaches to management, such as evaluation of new chemicals 14 or compounds 15 , new cultural approaches 1,4,19 , or the selection and development of resistant cultivars 7,8,18 , requires the use of whiteflies for virus transmission.…”

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confidence: 99%

Transmitting Plant Viruses Using Whiteflies

Polston

Capobianco

2013

JoVE

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Whiteflies, Hemiptera: Aleyrodidae, Bemisia tabaci, a complex of morphologically indistinquishable species 5 , are vectors of many plant viruses. Several genera of these whitefly-transmitted plant viruses (Begomovirus, Carlavirus, Crinivirus, Ipomovirus, Torradovirus) include several hundred species of emerging and economically significant pathogens of important food and fiber crops (reviewed by 9,10,16 ). These viruses do not replicate in their vector but nevertheless are moved readily from plant to plant by the adult whitefly by various means (reviewed by 2,6,7,9,10,11,17 ). For most of these viruses whitefly feeding is required for acquisition and inoculation, while for others only probing is required. Many of these viruses are unable or cannot be easily transmitted by other means. Therefore maintenance of virus cultures, biological and molecular characterization (identification of host range and symptoms) 3,13 , ecology 2,12 , require that the viruses be transmitted to experimental hosts using the whitefly vector. In addition the development of new approaches to management, such as evaluation of new chemicals 14 or compounds 15, new cultural approaches 1,4,19 , or the selection and development of resistant cultivars 7,8,18 , requires the use of whiteflies for virus transmission. The use of whitefly transmission of plant viruses for the selection and development of resistant cultivars in breeding programs is particularly challenging 7 . Effective selection and screening for resistance employs large numbers of plants and there is a need for 100% of the plants to be inoculated in order to find the few genotypes which possess resistance genes. These studies use very large numbers of viruliferous whiteflies, often several times per year.Whitefly maintenance described here can generate hundreds or thousands of adult whiteflies on plants each week, year round, without the contamination of other plant viruses. Plants free of both whiteflies and virus must be produced to introduce into the whitefly colony each week. Whitefly cultures must be kept free of whitefly pathogens, parasites, and parasitoids that can reduce whitefly populations and/or reduce the transmission efficiency of the virus. Colonies produced in the manner described can be quickly scaled to increase or decrease population numbers as needed, and can be adjusted to accommodate the feeding preferences of the whitefly based on the plant host of the virus.There are two basic types of whitefly colonies that can be maintained: a nonviruliferous and a viruliferous whitefly colony. The nonviruliferous colony is composed of whiteflies reared on virus-free plants and allows the weekly availability of whiteflies which can be used to transmit viruses from different cultures. The viruliferous whitefly colony, composed of whiteflies reared on virus-infected plants, allows weekly availability of whiteflies which have acquired the virus thus omitting one step in the virus transmission process.

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Tomato Yellow Leaf Curl Virus, a Whitefly-Borne Geminivirus of Tomatoes

Cited by 148 publications

References 66 publications

Interaction with host SGS3 is required for suppression of RNA silencing by tomato yellow leaf curl virus V2 protein

Interaction with host SGS3 is required for suppression of RNA silencing by tomato yellow leaf curl virus V2 protein

The capsid protein of tomato yellow leaf curl virus binds cooperatively to single-stranded DNA.

Transmitting Plant Viruses Using Whiteflies

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