The notion of mutual unbiasedness for coarse-grained measurements of quantum continuous variable systems is considered. It is shown that while the procedure of "standard" coarse graining breaks the mutual unbiasedness between conjugate variables, this desired feature can be theoretically established and experimentally observed in periodic coarse graining. We illustrate our results in an optics experiment implementing Fraunhofer diffraction through a periodic diffraction grating, finding excellent agreement with the derived theory. Our results are an important step in developing a formal connection between discrete and continuous variable quantum mechanics.Introduction. The ability to measure a system in an infinite number of non-commuting bases distinguishes the quantum world from classical physics. Wave-particle duality and more generally the complementarity principle are directly rooted in this feature of quantum mechanics. Though one can measure a quantum system in several distinct bases, uncertainty relations limit the amount of information that can be obtained. It is well known that projection onto an eigenstate of one basis reduces the information that can be obtained through or inferred about subsequent measurement in a different basis. The information is minimum for mutually unbiased bases (MUBs), for which all outcomes of the second measurement are equally likely, so that total uncertainty is always substantial (the sharpest uncertainty relations [1]) and most insensitive to input states [2]. MUBs play an important role in complementarity [3], quantum cryptography [4] and quantum tomography [5,6], are useful for certifying quantum randomness [7], and for detecting quantum correlations such as entanglement [8][9][10] and steering [11][12][13][14][15][16][17][18].