Tong-Xie-Yao-Fang alleviates diarrhea-predominant irritable bowel syndrome in rats via the GCN2/PERK-eIF2α-ATF4 signaling pathway

Zhang, Min; Zheng, Yijun; Li, Xia; Wu, Haomeng; Liu, Ping; Zhang, Kunli; Shi, Zhongfei; Lv, Mi; Wang, Fengyun; Tang, Xudong

doi:10.1016/j.phymed.2022.154350

Cited by 27 publications

(11 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Folic acid de ciency can lead to severe carbon metabolism abnormalities and lead to chronic disease and developmental disorders (58). Patients with IBS showed lower levels of lysine (59). Lysine is one of the essential amino acids in the human body.…”

Section: Discussionmentioning

confidence: 99%

Diarrhea Accompanies Intestinal Inflammation and Intestinal Mucosal Microbiota Dysbiosis during Fatigue Combined with a High-Fat Diet

Liu

Qiao

Cai

et al. 2023

Preprint

View full text Add to dashboard Cite

Objective: It was reported fatigue or a high-fat diet triggers gastrointestinal (GI) disorders, and intestinal microbiota may play central roles in GI disorders. Therefore, we investigated the association between the intestinal mucosal microbiota and the intestinal mucosal barrier from fatigue combined with a high-fat diet. Method: This study divided the Specific pathogen-free (SPF) male into the normal group (MCN) and the standing united lard group (MSLD). After establishing the model for 14 days, interleukin-6 (IL-6), interleukin-17 (IL-17), immunoglobulin A (sIgA), mucin 2 (Muc2), and intestinal mucosal microbiota were analyzed. Furthermore, the correlations between bacterial genera, inflammation, and immune function were measured. Result: The pathological analysis proved obvious damage to the small intestinal structure after fatigue combined with a high-fat diet. MSLD mice presented an increased trend of IL-6 and IL-17 and a decreased tendency of sIgA and Muc2, aggravating the injury of the intestinal mucus barrier and GI symptoms. Fatigue combined with a high-fat diet considerably decreased Limosilactobacillus reuteri, positively associated with Muc2 and negatively with IL-6. Conclusion: The interactions between Limosilactobacillus reuteriand intestinal inflammation might be involved in the process of intestinal mucosal barrier impairment in fatigue combined with high-fat diet-induced diarrhea.

show abstract

Section: Discussionmentioning

confidence: 99%

Diarrhea Accompanies Intestinal Inflammation and Intestinal Mucosal Microbiota Dysbiosis during Fatigue Combined with a High-Fat Diet

Liu

Qiao

Cai

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…This process was repeated until the weight of the feces no longer changed. Fecal water content was calculated as follows: feces water content (%) = (wet weight of feces + dry weight of feces)/ wet weight of feces×100% [ 12 ]).…”

Section: Methodsmentioning

confidence: 99%

“…Through this mechanism, it improves communication between the brain and intestines, coordination between upper and lower systems, emotional well-being, and normal qi movement. Overall, TCM provides a holistic approach to balance the bowels and relieve abdominal pain [ 12 – 14 ]. The above TCM theories coincide with modern medicine’s perspective of the brain-gut interaction.…”

Section: Introductionmentioning

confidence: 99%

Efficacy of Shu-yi-ning-chang decoction on IBS-D: Modulating Nr4a3 pathway to reduce visceral hypersensitivity

Guo,

Lu,

Yang

et al. 2024

PLoS ONE

View full text Add to dashboard Cite

Aim of the study To evaluate the therapeutic effect of SYNC in diarrhea irritable bowel syndrome (IBS-D) and explore its underlying mechanism through transcriptomic sequencing (RNA-Seq). Materials and methods A rat model of IBS-D was constructed to elucidate the effects of SYNC. Abdominal withdrawal reflex (AWR), fecal water content (FWC), and recording body weight were calculated to assess visceral sensitivity in rats. Histopathological changes in the colon and alterations in mast cell (MC) count were determined. Immunohistochemistry was employed to assess mast cell tryptase (MCT) expression in rat colons. Serum levels of corticotropin-releasing Hormone (CRH), interleukin-6 (IL-6), calcitonin gene-related peptide (CGRP), and 5-hydroxytryptamine (5-HT) were quantified using ELISA. RNA-Seq of colon tissue was performed, followed by Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses. Western blot analysis was conducted to quantify the expression levels of key proteins in the Nr4a3 pathway in the colon and hypothalamus tissues of rats. Results SYNC alleviated visceral hypersensitivity and mood disorders in rats with IBS-D. Moreover, it was positively correlated with its dosage and the observed effects, such as the enhancement of the colon’s mucosal lining condition and reduction in the number and activation of MCs within the model group. SYNC reduced the expression levels of factors related to the brain-gut axis and inflammatory markers in the bloodstream. RNA-Seq analysis indicated that SYNC down-regulated the expression of Nr4a3 and PI3K. These SYNC-targeted genes primarily played roles in immune regulation and inflammatory responses, correlating with the modulation of Nr4a3 and the PI3K/AKT pathway. Western blot analysis further confirmed SYNC’s influence on inflammation-related MC activation by downregulating key proteins in the Nr4a3/PI3K pathway. Conclusions SYNC inhibited mast cell activation and attenuated visceral hypersensitivity in the colon tissues of IBS-D rats. These effects were mediated by the Nr4a3/PI3K signaling pathway.

show abstract

“…A composição desequilibrada da microbiota auxilia na inflamação intestinal descontrolada, comprometendo a barreira intestinal e o sistema imunológico. Ademais, os desgastes que os radicais livres acarretam para o tecido e a morte celular por apoptose, contribuem para a inflamação e até mesmo para ulcerações (LUU et al, 2022;PAPADAKIS, 2019;ZHANG et al, 2022;WANG et al, 2022). As DII se tornaram bastante incidentes em países cuja industrialização é relativamente recente, o que reforça os estudos sobre o papel da disbiose no desenvolvimento de patologias intestinais inflamatórias, podendo contribuir para o aumento da incidência de DII-CCR em um futuro não muito distante (MULLER et al, 2020).…”

Section: Objectiveunclassified

Implementação da educação em saúde na prevenção de neoplasias penianas: revisão narrativa

Pedro¹,

Lima²,

Assis³

et al. 2022

O Cenário Pós-Pandêmico No Brasil E No Mundo: Lições Aprendidas E Reflexões Sobre a Saúde Pública E a Importância Da Adoção De

View full text Add to dashboard Cite

Teelessaúde e Atenção Primária à Saúde na p .... OBJETIVO: Descrever a importância da implementação da educação em saúde na prevenção de neoplasias penianas. MÉTODOS: Trata-se de uma revisão narrativa da literatura, realizada entre os meses de junho e julho de 2022, através dos bancos de dados da Biblioteca Virtual em Saúde. Utilizados os Descritores em Ciências da Saúde (DeCS) em cruzamento com o operador booleano AND. Estabelecidos os critérios de inclusão e exclusão, desta forma, foram selecionados cinco artigos para compor o estudo. RESULTADOS: Os principais resultados evidenciam a diminuição da incidência da neoplasia peniana conforme a implementação de ações educacionais, desta forma constata-se que a educação em saúde é uma ferramenta essencial na promoção da saúde do homem, em relação a saúde sexual na prevenção de neoplasias, e o profissional da enfermagem destaca-se neste processo. Em relação ao perfil social dos pacientes acometidos pela neoplasia destaca-se a baixa escolaridade, evidenciando na importância da assistência adequada e compreensível para todos os níveis sociais. CONCLUSÃO: A educação é um instrumento imprescindível na promoção da saúde, e desta forma faz-se necessário a formulação de protocolos assistenciais, além da inclusão de componentes relacionados à saúde homem, na formação acadêmica do profissional. PALAVRAS-CHAVE: Neoplasias penianas. Educação em saúde. Saúde do homem. OBJECTIVE:To describe the importance of implementing health education in the prevention of penile neoplasms. METHODS: This is a narrative review of the literature, carried out between June and July 2022, through the Virtual Health Library databases. Descriptors in Health Sciences (DeCS) were used in crossing with the Boolean operator AND. Having established the inclusion and exclusion criteria, in this way, five articles were selected to compose the study. RESULTS: The main results show the decrease in the incidence of penile neoplasia, in relation to the social profile of patients affected by the neoplasia, identified: Married, white, heterosexual, average age -above 40 to 65 years, low education and retirees; Health education proves to be an essential tool in the promotion of men's health, in relation to sexual health in the prevention of neoplasms, and the nursing professional stands out in this process. CONCLUSION: Education is an essential tool in health promotion, and thus, it is necessary to formulate care protocols, in addition to the inclusion of components related to men's health, in the professional's academic training.

show abstract

Tong-Xie-Yao-Fang alleviates diarrhea-predominant irritable bowel syndrome in rats via the GCN2/PERK-eIF2α-ATF4 signaling pathway

Cited by 27 publications

References 48 publications

Diarrhea Accompanies Intestinal Inflammation and Intestinal Mucosal Microbiota Dysbiosis during Fatigue Combined with a High-Fat Diet

Diarrhea Accompanies Intestinal Inflammation and Intestinal Mucosal Microbiota Dysbiosis during Fatigue Combined with a High-Fat Diet

Efficacy of Shu-yi-ning-chang decoction on IBS-D: Modulating Nr4a3 pathway to reduce visceral hypersensitivity

Implementação da educação em saúde na prevenção de neoplasias penianas: revisão narrativa

Contact Info

Product

Resources

About