Tonggyu-tang, a traditional Korean medicine, suppresses pro-inflammatory cytokine production through inhibition of MAPK and NF-κB activation in human mast cells and keratinocytes

Kim, Hyo In; Hong, Se Hyang; Ku, Jin Mo; Kang, Seong Joon; Kim, Tai Young; Shin, Yong Cheol; Ko, Seong‐Gyu

doi:10.1186/s12906-017-1704-5

Cited by 23 publications

(18 citation statements)

References 43 publications

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“…Some studies also showed that herbal decoctions could reduce the release of inflammatory factors in allergic diseases and keratinocytes by inhibiting mitogen-activated protein kinase and NF-jB pathways. 32,33 In addition, the decoction composed of AM and angelica sinensis could inhibit the NF-kB pathway and reduce allergic inflammation of the skin. 34 In our study, OVA significantly increased the mRNA expression of p65, while AM significantly decreased this trend.…”

Section: Discussionmentioning

confidence: 99%

Effect of Astragalus membranaceus in Ovalbumin-Induced Allergic Rhinitis Mouse Model

Zhong

Jin-Tao

Liu

et al. 2019

Am J Rhinol�Allergy

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Background Astragalus membranaceus (AM), a traditional Chinese medicine, has been used to treat allergic diseases, but the mechanism for treating allergic rhinitis (AR) remains unclear. Objective The purpose of this study was to look at the anti-inflammatory effect of AM on AR and the mechanism of anti-allergy. Methods The mouse model of AR was induced by ovalbumin. Allergic symptoms, number of eosinophils in nasal mucosa, and levels of inflammatory cells in nasal lavage fluid were analyzed. We explored the serum immunoglobulin E (IgE), interleukin-4 (IL-4), IL-5, IL-13, interferon-γ (IFN-γ), and IL-10. Besides, the relative mRNA of IL-4, IL-5, and IL-13 was also detected in nasal mucosa tissue. The proportion of CD4+ CD25+ Foxp3+ T cells in the spleen and nasal lymphoid tissue were analyzed. The mRNA levels of nuclear factor-kappa B p65 (NF-κB p65) and inhibitory kappa B alpha (IκBα), as well as NF-κB p65 DNA binding activity, were tested. We also measured the protein levels of NF-κB p65 and p-NF-κB p65 in nasal mucosa. Results AM could reduce the number of eosinophils in the nasal mucosa and decrease the levels of inflammatory cells in nasal lavage fluid. The serum IgE, IL-4, IL-5, and IL-13 were also decreased, while levels of IFN-γ and IL-10 were increased. The relative mRNA of IL-4, IL-5, and IL-13 was decreased by AM. AM increased the proportion of CD4+ CD25+ Foxp3+ T cells in the spleen and nasal lymphoid tissue. In addition, AM could reduce the activity of NF-kB by inhibiting the mRNA expression and DNA binding activity of NF-κB p65. However, AM had no significant effect on mRNA of IκBα. Above all, AM could reduce the p-NF-κB p65 protein expression of nasal mucosa. Conclusions AM could reduce the secretion of inflammatory cytokines by increasing the level of CD4+ CD25+ Foxp3+ T cells and inhibiting the activation of the NF-κB.

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Section: Discussionmentioning

confidence: 99%

Effect of Astragalus membranaceus in Ovalbumin-Induced Allergic Rhinitis Mouse Model

Zhong

Jin-Tao

Liu

et al. 2019

Am J Rhinol�Allergy

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“…MAPK can transmit signals from receptors on the cell surface to DNA in the nucleus and participate in biological processes, such as cell growth, death, and cell cycle, and also in the regulation of pathological processes, such as inflammation and stress response. As a member of the MAPK family, MAPK3 plays an important role in the process of proliferation, differentiation, and formation of inflammatory cells [ 33 , 34 ]. PTGS2 is an inducible immediate response gene that is not expressed in most cells under normal physiological conditions, but during pathological reactions such as inflammation or tumors, the expression of PTGS2 is rapidly upregulated, producing a large amount of prostaglandins.…”

Section: Discussionmentioning

confidence: 99%

Network Pharmacology Identifies the Mechanisms of Sang‐Xing‐Zhi‐Ke‐Fang against Pharyngitis

Deng

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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Background. Sang-Xing-Zhi-Ke-Fang (SXZKF) demonstrates good therapeutic effect against pharyngitis. Nevertheless, the pharmacological mechanism underlying its effectiveness is still unclear. Objective. To investigate the underlying mechanisms of SXZKF against pharyngitis using network pharmacology method. Methods. Bioactive ingredients of SXZKF were collected and screened using published literature and two public databases. Using four public databases, the overlapping genes between these bioactive compound-related and pharyngitis-related genes were identified by Venn diagram. Protein-protein interaction (PPI) was obtained using “Search Tool for the Retrieval of Interacting Genes (STRING)” database. “Database for Annotation, Visualization, and Integrated Discovery ver. 6.8 (DAVID 6.8)” was used to perform Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis to explore the molecular mechanisms of SXZKF against pharyngitis. Finally, Cytoscape 3.7.2 software was used to construct and visualize the networks. Result. A total of 102 bioactive compounds were identified. Among them, 886 compounds-related and 6258 pharyngitis-related genes were identified, including 387 overlapping genes. Sixty-three core targets were obtained, including ALB, PPARγ, MAPK3, EGF, and PTGS2. Signaling pathways closely related to mechanisms of SXZKF for pharyngitis were identified, including serotonergic synapse, VEGF signaling pathway, Fc epsilon RI signaling pathway, Ras signaling pathway, MAPK signaling pathway, and influenza A. Conclusion. This is the first identification of in-depth study of SXZKF against pharyngitis using network pharmacology. This new evidence could be informative in providing new support on the clinical effects of SXZKF on pharyngitis and for the development of personalized medicine for pharyngitis.

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“…MAPKs are closely associated with the expression of inflammatory cytokines and chemokines upon pathogen challenge. The phosphorylation of ERK, JNK and p38 MAPKs can also activate NF-Κb ( 26 ). Previous studies have suggested that Evo inhibits the migration and invasiveness of NPC cells accounting for the inactivation of NF-κB and MAPKs ( 27 ).…”

Section: Discussionmentioning

confidence: 99%

Evodiamine alleviates severe pneumonia induced by methicillin-susceptible Staphylococcus�aureus following cytomegalovirus reactivation through suppressing NF-κB and MAPKs

Chen

Zhou

2018

Int J Mol Med

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Viral and bacterial severe pneumonia are leading causes of mortality across the globe. Evodiamine (Evo), a botanical alkaloid, has anti-inflammatory and antibacterial properties. In the present study, the effect of Evo on severe pneumonia induced by methicillin-susceptible Staphylococcus aureus (MSSA) following cytomegalovirus (CMV) reactivation, and its mechanism, were evaluated. In vitro, the protein and mRNA expression levels of inflammatory cytokines were determined by enzyme-linked immunosorbent assay and reverse transcription-quantitative polymerase chain reaction analysis, respectively. The expression levels of associated proteins of the nuclear factor (NF)-κB and mitogen-activated protein kinase (MAPK) signaling pathways were measured by western blot analysis. In vivo, mortality rate, weight loss, histological changes, lung bacteria count, inflammatory cytokines, and the expression proteins of associated with the NF-κB and MAPK signaling pathways were examined. The results revealed that Evo dose-dependently reduced the protein and mRNA expression levels of tumor necrosis factor (TNF)-α, interleukin (IL)-6 and IL-1β, and inhibited the levels of phosphorylated (p-) inhibitor of NF-κBα, p-extracellular signal-regulated kinase, p-c-Jun N-terminal kinase and p-p38, and decreased the nuclear trans-location of NF-κB/p65 in BEAS-2B cells infected with MSSA. Furthermore, Evo markedly improved survival rate, decreased body weight loss and bacterial count, and attenuated lung histological alterations and the levels of inflammatory factors. In addition, the NF-κB and MAPK signaling pathways were significantly inhibited. Taken together, Evo effectively alleviated pneumonia via the NF-κB and MAPK pathways and may be a potential therapeutic agent for treating severe pneumonia.

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Tonggyu-tang, a traditional Korean medicine, suppresses pro-inflammatory cytokine production through inhibition of MAPK and NF-κB activation in human mast cells and keratinocytes

Cited by 23 publications

References 43 publications

Effect of Astragalus membranaceus in Ovalbumin-Induced Allergic Rhinitis Mouse Model

Effect of Astragalus membranaceus in Ovalbumin-Induced Allergic Rhinitis Mouse Model

Network Pharmacology Identifies the Mechanisms of Sang‐Xing‐Zhi‐Ke‐Fang against Pharyngitis

Evodiamine alleviates severe pneumonia induced by methicillin-susceptible Staphylococcus�aureus following cytomegalovirus reactivation through suppressing NF-κB and MAPKs

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