Top-down synthesized TiO2 nanowires as a solid matrix for surface-assisted laser desorption/ionization time-of-flight (SALDI-TOF) mass spectrometry

Kim, Jo Il; Park, Jong Min; Hwang, Seung Ju; Kang, Min Jung; Pyun, Jae Chul

doi:10.1016/j.aca.2014.05.041

Cited by 33 publications

(42 citation statements)

References 44 publications

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“…Organic dispersive liquid medium was eliminated to completely reduce interferences in the low mass range below m/z 1000. TiO 2 thin films 13,14 , nanoparticles 15,16 , nanotubes 17,18 , and nanowires 19 were developed as very effective materials for SALDI-MS.…”

Section: Introductionmentioning

confidence: 99%

“…20,21 However, Kim et al have recently presented that TiO 2 nanowires can be easily synthesized through the modified hydrothermal process simply by changing aqueous solutions at ambient atmospheric conditions. 19 Nanowires can be synthesized from the planar Ti plate, which is a more attractive approach for IMS applications. Potentially, TiO 2 nanowire surface can be used as a solid LDI-IMS substrate, although it has a notable superhydrophilic character, in which water droplets deposited on its surface spreads completely and deteriorate the sensitivity, as well as the lateral resolution of subsequent IMS.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Functionalized Titanium Oxide Nanowire Substrate for Surface-Assisted Laser Desorption/ionization Imaging Mass Spectrometry

Ep¹,

H²,

Hsu³

et al. 2019

Preprint

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Imaging mass spectrometry (IMS) is a powerful technique that enables analysis of various molecular species at a high spatial resolution with low detection limits. In contrast to the standard matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) approach, surface-assisted laser desorption/ionization (SALDI) is more effective in the detection of small molecules due to the absence of interfering background signals in low m/z ranges. We developed a functionalized TiO2 nanowire as a solid substrate for IMS of low-molecular-weight species in biological specimens. We prepared TiO2 nanowires using the inexpensive modified hydrothermal process and subsequently functionalized it chemically with various silane analogs to overcome the problem of superhydrophilicity of the substrate. Chemical modification changed the selectivity of imprinting of samples deposited on the surface of the plate and thus improved the detection limits. Due to the enhanced performance, the functionalized TiO2 nanowire substrate could be successfully used for imaging of complex native samples. We applied our new substrate to image distribution of the secondary metabolites in (1) petal of the medicinal plant Catharanthus roseus and (2) microbial co-culture of Burkholderia cenocepacia 869T2 vs Phellinus noxius. We observed that secondary metabolites are distributed heterogeneously in a petal, which is consistent with previous results reported for the C. roseus plant leaf and stem. We verified the semi-quantitative capabilities of the imprinting/imaging approach by comparing results using standard LC-MS analysis of the plant extracts. Several bacteria-related metabolites produced by B. cenocepacia 869T2 in presence of P. noxius, which were unable to be detected by MALDI-MS approach, were revealed by our newly developed approach. This suggested that the functionalized TiO2 nanowire substrates-based SALDI is a powerful technique complementary to MALDI-MS.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Functionalized Titanium Oxide Nanowire Substrate for Surface-Assisted Laser Desorption/ionization Imaging Mass Spectrometry

Ep¹,

H²,

Hsu³

et al. 2019

Preprint

View full text Add to dashboard Cite

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“…In the last decade, One-dimensional (1D) TiO 2 including nanorods, nanowires, and nanotubes have been extensively investigated since it confirmed to be superior photoelectrochemical (PEC) properties to other dimensional TiO 2 nanostructures. [1][2][3][4][5][6] Among them, single crystal TiO 2 nanorods (TNRs) or nanowires further offer direct electrical pathways for photogenerated electrons and could increase the oriented charge separation and the electron transport rate, [7][8][9][10][11] and the length of nanorods is a key factor influencing the charge collection efficiency. Yang et al reported that, with increasing the length of the single crystalline rutile TiO 2 nanorods to about 1.8 μm, the photocurrent density was close to the maximum.…”

Section: Introductionmentioning

confidence: 99%

Visible light photoelectrochemical properties of a hydrogenated TiO₂ nanorod film and its application in the detection of chemical oxygen demand

et al. 2015

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“…Top down synthesized TiO 2 nanowires were presented as an ideal solid matrix for LDI-TOF MS to analyze small biomolecules by Kim et al [94]. The TiO 2 nanowires were synthesized as arrays using a modified hydrothermal process directly on the surface of a Ti plate.…”

Section: Nanomaterial-assisted Ldi For the Analysis Of Small Biolomentioning

confidence: 99%

Nanomaterials as Assisted Matrix of Laser Desorption/Ionization Time-of-Flight Mass Spectrometry for the Analysis of Small Molecules

Yang

et al. 2017

Nanomaterials

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Matrix-assisted laser desorption/ionization (MALDI), a soft ionization method, coupling with time-of-flight mass spectrometry (TOF MS) has become an indispensible tool for analyzing macromolecules, such as peptides, proteins, nucleic acids and polymers. However, the application of MALDI for the analysis of small molecules (<700 Da) has become the great challenge because of the interference from the conventional matrix in low mass region. To overcome this drawback, more attention has been paid to explore interference-free methods in the past decade. The technique of applying nanomaterials as matrix of laser desorption/ionization (LDI), also called nanomaterial-assisted laser desorption/ionization (nanomaterial-assisted LDI), has attracted considerable attention in the analysis of low-molecular weight compounds in TOF MS. This review mainly summarized the applications of different types of nanomaterials including carbon-based, metal-based and metal-organic frameworks as assisted matrices for LDI in the analysis of small biological molecules, environmental pollutants and other low-molecular weight compounds.

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Top-down synthesized TiO2 nanowires as a solid matrix for surface-assisted laser desorption/ionization time-of-flight (SALDI-TOF) mass spectrometry

Cited by 33 publications

References 44 publications

Functionalized Titanium Oxide Nanowire Substrate for Surface-Assisted Laser Desorption/ionization Imaging Mass Spectrometry

Functionalized Titanium Oxide Nanowire Substrate for Surface-Assisted Laser Desorption/ionization Imaging Mass Spectrometry

Visible light photoelectrochemical properties of a hydrogenated TiO₂ nanorod film and its application in the detection of chemical oxygen demand

Nanomaterials as Assisted Matrix of Laser Desorption/Ionization Time-of-Flight Mass Spectrometry for the Analysis of Small Molecules

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Top-down synthesized TiO2 nanowires as a solid matrix for surface-assisted laser desorption/ionization time-of-flight (SALDI-TOF) mass spectrometry

Cited by 33 publications

References 44 publications

Functionalized Titanium Oxide Nanowire Substrate for Surface-Assisted Laser Desorption/ionization Imaging Mass Spectrometry

Functionalized Titanium Oxide Nanowire Substrate for Surface-Assisted Laser Desorption/ionization Imaging Mass Spectrometry

Visible light photoelectrochemical properties of a hydrogenated TiO2 nanorod film and its application in the detection of chemical oxygen demand

Nanomaterials as Assisted Matrix of Laser Desorption/Ionization Time-of-Flight Mass Spectrometry for the Analysis of Small Molecules

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Product

Resources

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Visible light photoelectrochemical properties of a hydrogenated TiO₂ nanorod film and its application in the detection of chemical oxygen demand