Topical application of glycolic acid suppresses the UVB induced IL-6, IL-8, MCP-1 and COX-2 inflammation by modulating NF-κB signaling pathway in keratinocytes and mice skin

Tang, Sheau‐Chung; Liao, Pei-Yun; Hung, Sung-Jen; Ge, Jheng-Siang; Chen, Shiou-Mei; Lai, Ji-Ching; Hsiao, Yu‐Ping; Yang, Jen‐Hung

doi:10.1016/j.jdermsci.2017.03.004

Cited by 84 publications

(64 citation statements)

References 49 publications

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“…TNF-α, MCP-1, IL-6, and IL-8 are well characterized NF-κB transcriptional targets ( Tang et al, 2017 ). We therefore assessed the impact of EISO treatment on NF-κB activation by measuring p-p65 levels in LPS-stimulated DF, BEAS-2B, A549, and THP-1 cells.…”

Section: Resultsmentioning

confidence: 99%

East Indian Sandalwood Oil Is a Phosphodiesterase Inhibitor: A New Therapeutic Option in the Treatment of Inflammatory Skin Disease

Sharma

Levenson²,

Browning

et al. 2018

Front. Pharmacol.

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Cyclic adenosine monophosphate phosphodiesterases (PDEs) regulate pro-inflammatory cytokine production. One isoform, PDE4, is overactive in chronic relapsing inflammatory skin diseases: psoriasis and eczema/atopic dermatitis, and in several cancers. East Indian sandalwood oil (EISO) has significant anti-inflammatory properties. Here, we report that 75% of pediatric eczema/atopic dermatitis patients treated with topical EISO formulations achieved a >50% reduction in their Eczema Area and Severity Index score. EISO treatment of a psoriasis model reduced PDE4 expression and reversed histopathology. EISO directly inhibited PDE enzymatic activity in vitro . In lipopolysaccharide-stimulated human dermal fibroblast, BEAS-2B, A549, and THP-1 cells, EISO suppressed total cellular PDE activity, PDE4, and 7 transcript levels, nuclear factor kappa B (NF-κB) activation, and pro-inflammatory cytokines/chemokine production. These results suggest that EISO anti-inflammatory activity is mediated through suppressing PDE activity, thus facilitating cAMP-regulated inhibition of NF-κB and indicate EISO as an attractive natural therapeutic for chronic and acute inflammatory disorders.

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Section: Resultsmentioning

confidence: 99%

East Indian Sandalwood Oil Is a Phosphodiesterase Inhibitor: A New Therapeutic Option in the Treatment of Inflammatory Skin Disease

Sharma

Levenson²,

Browning

et al. 2018

Front. Pharmacol.

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“…as IL-1, IL-6, IL-7, IL-8, IL-12, IL-15, TNF-α, monocyte chemoattractant protein (MCP)-1, and granulocytemacrophage colony-stimulating factor (GM-CSF), in keratinocytes in the skin 24,25 . These cytokines are associated with the development of various inflammatory skin diseases, such as psoriasis and atopic dermatitis [46][47][48] .…”

Section: Discussionmentioning

confidence: 99%

“…Upon exposure to UVB radiation in the epidermis, several types of cells, especially keratinocytes, produce proinflammatory cytokines, such as IL-1α, IL-6, IL-8 and tumor necrosis factor (TNF)-α, leading to skin inflammation [23][24][25] . To determine whether lipin-1 is involved in the generation of proinflammatory responses to UVB radiation, NHEKs were transfected with vectors containing lipin-1 cDNA or the pCMV-AC empty vector as a negative control, stimulated with UVB radiation and analyzed to determine changes in the levels of proinflammatory factors using the Raybio human cytokine assay.…”

Section: Lipin-1 Regulates Uvb-induced Il-6 and Il-8 Productionmentioning

confidence: 99%

UVB-dependent inhibition of lipin-1 protects against proinflammatory responses in human keratinocytes

et al. 2020

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Lipin-1 is an Mg 2+-dependent phosphatidate phosphatase (PAP1) that catalyzes a critical step in the synthesis of glycerophospholipids and is also a cotranscriptional regulator. The role of lipin-1 in the regulation of inflammatory responses has been extensively studied in various cell types but not in skin cells. In the present study, the function of lipin-1 in UVB-induced proinflammatory responses was assessed in normal human epidermal keratinocytes (NHEKs). UVB radiation downregulated lipin-1 expression. Lipin-1 inhibition was mediated by UVB-dependent sterol-response element binding protein-1 (SREBP-1) inhibition. The UVB-dependent inhibition of lipin-1 and SREBP-1 was mediated by AMPK activation. UVB-induced activation of JNK was dependent on AMPK activation and mediated lipin-1 inhibition. Prevention of UVB-mediated lipin-1 repression by introducing a lipin-1 expression vector stimulated IL-6 and IL-8 production, suggesting that lipin-1 inhibition attenuates UVB-induced IL-6 and IL-8 production. The downregulation of lipin-1 ameliorated UVB-induced NF-ĸB phosphorylation, which might be attributed to the suppression of UVB-induced accumulation of free fatty acids (FFAs). Pharmacological inhibition of PAP1 with propranolol suppressed UVB-induced production of IL-6 and IL-8 in NHEKs and reconstituted human skin models. Taken together, lipin-1 is downregulated by exposure to UVB radiation, which confers protection against UVB-induced proinflammatory responses; therefore, the inhibition of lipin-1 is a potential strategy for photoaging.

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“…QDG treatment in keratinocytes not only reduced the phosphorylation of p38, JNK, and ERK in a concentration-dependent manner, but also decreased NF-κB levels and the generation of chronic inflammatory disease factors due to UVB irradiation. According to the report, glycolic acid induced by UVB stimulation inhibits overexpressed factors by UVB-mediated NF-κB signaling in the HaCaT cells, suggesting that it inhibits inflammation [ 49 ]. Our study also suggests that QDG has an anti-inflammatory effect on the overexpression of inflammatory factors by UVB stimulation.…”

Section: Discussionmentioning

confidence: 99%

Anti-Inflammatory and Skin-Moisturizing Effects of a Flavonoid Glycoside Extracted from the Aquatic Plant Nymphoides indica in Human Keratinocytes

Kim

Park

et al. 2018

Molecules

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Nymphoides indica, an aquatic plant, is used as folk medicine in some countries. Our previous study demonstrated that the methanol extract of N. indica inhibited the activity of tyrosinases, tyrosine related protein (TRP)1 and TRP2, and microphthalmia-associated transcription factor, as well as the activity of protein kinase A, by effectively inhibiting cyclic adenosine monophosphate. Although the biological activities of N. indica extract have been reported, there are no reports on the skin bioactivity of the main compound(s) on human keratinocytes. This study investigated the anti-inflammatory and moisturizing effects of quercetin 3,7-dimethyl ether 4′-glucoside (QDG) isolated from N. indica. In brief, ultraviolet B irradiated keratinocytes were pretreated with different concentrations of QDG, and the effects of QDG on various inflammatory markers were determined. QDG significantly inhibited inflammation-related cytokines and chemokines and enhanced the activation of skin barrier factors. Additionally, QDG also attenuated phosphorylation inhibition of the upstream cytokines and nuclear factor-κB expression. These results suggest that QDG isolated from N. indica may serve as a potential source of bioactive substances for chronic inflammatory skin diseases.

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Topical application of glycolic acid suppresses the UVB induced IL-6, IL-8, MCP-1 and COX-2 inflammation by modulating NF-κB signaling pathway in keratinocytes and mice skin

Abstract: We clarified the molecular mechanism of GA protection against UVB-induced inflammation by modulating NF-κB signaling pathways and determined the optimal concentration of GA in mice skin exposed to UVB irradiation.

Cited by 84 publications

References 49 publications

East Indian Sandalwood Oil Is a Phosphodiesterase Inhibitor: A New Therapeutic Option in the Treatment of Inflammatory Skin Disease

East Indian Sandalwood Oil Is a Phosphodiesterase Inhibitor: A New Therapeutic Option in the Treatment of Inflammatory Skin Disease

UVB-dependent inhibition of lipin-1 protects against proinflammatory responses in human keratinocytes

Anti-Inflammatory and Skin-Moisturizing Effects of a Flavonoid Glycoside Extracted from the Aquatic Plant Nymphoides indica in Human Keratinocytes

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