Topical Application of <i>Rosa multiflora</i> Root Extract Improves Atopic Dermatitis-Like Skin Lesions Induced by Mite Antigen in NC/Nga Mice

Abstract

The roots of Rosa multiflora THUNB. (RM) has been used in oriental traditional medicines as remedies for scabies, rheumatic arthralgia and stomatitis which were practicably related with today's inflammatory and allergic diseases. In the present study, we evaluated whether RM root extract (RME) and its major constituent, 2-(3,4-dihydroxyphenyl)-6-(4-hydroxyphenyl)-8-(2,4-dihydroxyphenyl)-2,3-trans-6,7-cis-7,8-trans-3,4,7,8-tetrahydro-2H,6H-pyrano[2,3-f ] chromene-3,7,9-triol (RM-3) belongs to condensed tannins, improve atopic dermatitis (AD)-like skin lesions in NC/Nga mice induced by mite antigen. Topical application of RME as well as RM-3 improved skin severity and suppressed mRNA levels of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) on skin tissues, in addition, significantly reduced T helper 2 (Th2) immune responses via interleukin 10 (IL-10) up-regulation. Thus, RME, contains lots of condensed tannins such as RM-3 which possesses potent anti-inflammtory and immune-modulatory effects, may be useful for treatment of skin allergies and can be developed as new alternative herbal therapy against AD.Key words Rosa multiflora; Rosaceae; condensed tannin; proanthocyanidin; phlobatannin; atopic dermatitis Atopic dermatitis (AD) is a chronic inflammatory skin disease characterized by pruritic and eczematous skin lesion. This disease primarily occurs in infants and children, and the incidence is gradually increasing in developing as well as developed countries. Although the cause of AD has been still unclear, it has been understood as immune disorder characterized by increased immunoglobulin E (IgE) and T helper 2 (Th2) cytokine levels as well as presence of blood eosinophilia.1) Meanwhile, topical corticosteroids have been widely used as the main therapeutic strategy against AD, however they could only be used for short periods of time and in limited skin regions because of serious adverse effects such as aggravation and recurrence.2) Therefore, the demands of alternative therapies for AD have been increasing.Rosa multiflora THUNB. (RM), called as multiflora rose, baby rose, many-flowered rose and seven-sisters rose, is native to Eastern Asia. While RM has been used as rootstock for ornamental roses in Western, all parts of RM has been known as folk medicine in Eastern. Especially, hips have been widely used as diuretics and also registered on Korean and Japanese Pharmacopoeia. Meanwhile, root of RM have been rarely researched in recent year although it has been traditionally used for rheumatism, scabies and stomatitis.3) In our previous works, condensed tannins including novel stereoisomers (RM-1, 2 and 3) as well as catechin (CAT), and procyanidin B-3 (ProB3) were isolated from the roots of RM, and RM-1 and ProB3 showed to improve AD-like skin lesion in vivo. 4,5) As part of the research for development of herbal new drug, the present study was conducted to evaluate therapeutic effects of RM root extract (RME) as well as additional main compound, RM-3, belongs to phlobatannin which is a kind ...

Therapeutic Applications of Rose Hips from Different Rosa Species

“…Of the different components that can be found in Rosa multiflora roots, the most likely candidate to act against AD is the condensed tannin RM-3. RM-3 is the most abundant phytochemical in Rosa multiflora root extract, and Park et al [177] proved that this isolated tannin reproduced the effects previously observed for the whole extract.…”

“…Topical application of extracts from Rosa multiflora root extracts improved AD-like injury in mice [177]. Anti-AD activity of Rosa multiflora seems to be related to its anti-inflammatory properties, since application of extracts reduced mRNA levels of inflammatory mediators cyclooxygenase 2 (COX-2) and inducible Nitric Oxide Synthase (iNOS).…”

The Potential of Plant Phenolics in Prevention and Therapy of Skin Disorders

“…Hence, natural immune modulators from herbal extracts or derivatives may be useful for treating AD symptoms. Such modulators include 7,8,4’-trihydroxyisoflavone (a metabolite of soy daidzin), flavonoids from Averrhoa carambola L. [ 277 , 278 ], eriodictyol (also a flavonoid) from Acer mono and Eriodictyon californicum [ 279 ], and tannins from Rosa multiflora [ 280 ]. In their research on apigenin, Hou et al revealed that this compound facilitates epidermal permeability barrier by stimulating epidermal differentiation, lipid synthesis and secretion, as well as cutaneous antimicrobial peptide production [ 281 ].…”

Tannic acid modulates NFκB signaling pathway and skin inflammation in NC/Nga mice through PPARγ expression

“…It has strong antioxidant, anti-inflammatory, anticarcinogenic and antimutagenic activities [11]. In addition, the condensed tannin attenuates inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)2 expression in AD NC/Nga mouse model [12]. Another similar study reported that two condensed tannins effectively suppress Th2 cytokines in AD mice [13].…”