Topical Application of TAT‐Superoxide Dismutase in Acupoints LI 20 on Allergic Rhinitis

Abstract

Reactive oxygen species are products of cellular metabolism and assigned important roles in biomedical science as deleterious factors in pathologies. In fact, some studies have shown that the therapeutic benefits of taking antioxidants were limited and the potential for therapeutic intervention remains unclear. New evidences showed that ROS have some ability of intercellular transportation. For treating allergic rhinitis, as a novel intracellular superoxide quencher, TAT-SOD applied to acupoints LI 20 instead of directly to nasal cavity can be used to test that. TTA group apply TAT-SOD cream prepared by adding purified TAT-SOD to the vehicle cream to acupoints LI 20, while placebo group used the vehicle cream instead. TTN group applied the same TAT-SOD cream directly to nasal cavity three times daily. Symptom scores were recorded at baseline and days 8 and 15. For the overall efficacy rate, TTA group was 81.0%, while placebo group was 5.9% and TTN was 0%. Malondialdehyde levels decreased observably in TTA group, and superoxide dismutase, catalase, and glutathione peroxidase levels remained basically unaffected. Enzymatic scavenging of the intracellular superoxide at acupoints LI 20 proved to be effective in treating allergic rhinitis, while no improvement was observed with the placebo group and TTN group.

Effects of TAT-SOD at Acupoints on Essential Hypertension by Monitoring Meridians Electrical Potential

“…The enzymatic scavenging of the intracellular superoxide at acupoints in the clinical setting achieved an efficacy in treating essential hypertension patients comparable to acupuncture. It further proves that the enzymatic scavenging of intracellular superoxide could generate acupuncture-like efficacy as was shown in the treatments of simple obesity [10,11] and allergic rhinitis [12] .…”

“…They were related to neither veins nor nerves but could be almost perfectly superimposable on a standard human acupuncture meridian network [9] . Moreover, topical application of TAT-Cu,Zn-superoxide dismutase (TAT-SOD) to acupoints were found to result in acupuncture-like action for treating simple obesity [10,11] and allergic rhinitis [12] . TAT-SOD is a recombinant protein of human liver Cu,Zn-superoxide dismutase (SOD) fused with TAT peptide, which enables the fusion protein to be delivered cross the cell membrane while maintaining SOD activities, making it an intracellular superoxide quenching enzyme [13] .…”

The Meridian Tropism and Classification of Red Yeast Rice Investigated by Monitoring Dermal Electrical Potential

“…ROS has a strong connection with REDOX potential (ORP) and antioxidant activity. Moreover, topical application of antioxidants to acupoints was found to result in an acupuncture-like action [ 10 , 11 , 22 , 23 ]. All those results point to a new applaudable hypothesis of meridians as channels of ROS and ROS modulation along meridians as the acupuncture mechanism.…”

Visualising reactive oxygen species in live mammals and revealing of ROS-related system

“…The frontal abdominal walls were excised from wild type mice (C57BL/6, group mito-cpYFP-) and transgenic mice (C57BL6-TgH, group mito-cpYFP+), respectively. A DC ampere meter was connected ( Fig 5A) [15,16]. Whilst connective tissue was confirmed to be the source of fluorescence, superoxide should be the ROS which effectively oxidizes the probes.…”