Topical delivery of growth hormone releasing peptide using liposomal systems: An in vitro study using hairless mouse skin

Fleisher, David; Niemiec, S. M.; Oh, Choon; Hu, Zhilian; Ramachandran, Chandrasekharan; Weiner, Norman

doi:10.1016/0024-3205(95)02086-x

Cited by 26 publications

(17 citation statements)

References 8 publications

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“…There are some reports describing that liposomes have been used to enhance transfollicular and transdermal delivery of the low-/high-molecular weight and hydrophilic/lipophilic compounds (Fleisher et al, 1995;Li and Hoffman, 1996;Niemiec et al, 1997;Niemiec et al, 1995;Seiberg et al, 1997). However, we have confirmed that in vivo permeation of liposomes from the hair follicles into dermis/epidermis was not appeared under passive condition ( Supplementary Fig.…”

Section: Discussionsupporting

confidence: 69%

Noninvasive and persistent transfollicular drug delivery system using a combination of liposomes and iontophoresis

Kajimoto

Yamamoto

Watanabe

et al. 2011

International Journal of Pharmaceutics

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Iontophoresis is a promising technique for enhancing transdermal administration of charged drugs. However, conventional iontophoresis is not sufficient for effective delivery of large, hydrophilic, or electrically neutral molecules. In this study, we utilized charged liposomes as carriers, focused on a transfollicular route for delivery of the liposomes, and optimized iontophoretic conditions and lipid composition for this method in both in vitro and in vivo conditions.As a result, we identified the optimum condition (lipid composition:DOTAP/EPC/Chol=2:2:1, current supply: 0.45 mA/cm 2 , duration: 1 hr) for effective iontophoretic delivery of aqueous solution, which can not be transferred into the skin without charged liposomes. We also examined the pharmacological effects of iontophoresis of liposomes encapsulating insulin (INS-lipo) using a rat model of type I diabetes. Interestingly, iontophoresis of INS-lipo onto a diabetes rat skin resulted in a gradual decrease in blood glucose levels, with levels reaching 20% of initial values at 18 hr after administration. These lower blood glucose levels were maintained for up to 24 hr. Significant amount of insulin were also detected in plasma 18 hr after iontophoresis of INS-lipo. We succeeded in developing a non-invasive and persistent transfollicular drug delivery system that used a combination of liposomes and iontophoresis.

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Section: Discussionsupporting

confidence: 69%

Noninvasive and persistent transfollicular drug delivery system using a combination of liposomes and iontophoresis

Kajimoto

Yamamoto

Watanabe

et al. 2011

International Journal of Pharmaceutics

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“…10 More efficient delivery of these macromolecules was achieved compared with those obtainable with conventional phospholipid-based liposomes and conventional solutions and gels. [11][12][13][14] Further, it was found that clinically significant levels of macromolecular proteins could be delivered to the vicinity of target sites by our nonionic formulations. 15,16 Based on these studies we hypothesized that expression plasmid DNA could be substituted as the charged macromolecule in nonionic liposomal formulations.…”

Section: Introductionmentioning

confidence: 99%

Perifollicular Transgenic Expression of Human Interleukin-1 Receptor Antagonist Protein following Topical Application of Novel Liposome-Plasmid DNA Formulations In Vivo

Niemiec

Latta

Ramachandran

et al. 1997

Journal of Pharmaceutical Sciences

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Expression plasmid DNA for the human interleukin-1 receptor antagonist (IL-1ra) protein was formulated with nonionic:cationic (NC) liposomes or phosphatidylcholine:cationic (PC) liposomes and applied to the auricular skin of hamsters in single- and multiple-dose protocols. Confocal microscopy identified delivery of plasmid DNA proximal to perifollicular cells, and successful transfection of perifollicular cells was identified by immunohistochemistry and ELISA. Skin treated for 3 days with the NC liposomes had statistically significant levels of transgenic IL-1ra present for 5 days post-treatment. Expression of transgenic IL-1ra was specific to areas of skin treated with NC liposomes but not PC liposomes. The results indicate that the NC liposomes can deliver expression plasmid DNA to perifollicular cells and mediate transient transfection in vivo.

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“…Fleisher et al [55] reported that nonionic emulsions are potent enhancers of niosomal systems. Nonionic emulsions are composed of niosomes and isopropyl myristate at a 80: 20 ratio by weight followed by sonication for 2 min.…”

Section: Topical Delivery Of Niosomesmentioning

confidence: 99%

Liposomes and Niosomes as Topical Drug Delivery Systems

Choi

Maibach

2005

Skin Pharmacol Physiol

215

104

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The skin acts as a major target as well as a principle barrier for topical/transdermal (TT) drug delivery. The stratum corneum plays a crucial role in barrier function for TT drug delivery. Despite major research and development efforts in TT systems and the advantages of these routes, low stratum corneum permeability limits the usefulness of topical drug delivery. To overcome this, methods have been assessed to increase permeation. One controversial method is the use of vesicular systems, such as liposomes and niosomes, whose effectiveness depends on their physicochemical properties. This review focuses on the effect of liposomes and niosomes on enhancing drug penetration, and defines the effect of composition, size and type of the vesicular system on TT delivery.

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Topical delivery of growth hormone releasing peptide using liposomal systems: An in vitro study using hairless mouse skin

Cited by 26 publications

References 8 publications

Noninvasive and persistent transfollicular drug delivery system using a combination of liposomes and iontophoresis

Noninvasive and persistent transfollicular drug delivery system using a combination of liposomes and iontophoresis

Perifollicular Transgenic Expression of Human Interleukin-1 Receptor Antagonist Protein following Topical Application of Novel Liposome-Plasmid DNA Formulations In Vivo

Liposomes and Niosomes as Topical Drug Delivery Systems

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