Topical delivery of vismodegib using ablative fractional laser and micro‐emulsion formulation in vitro

Olesen, Uffe H.; Clergeaud, Gael; Lerche, Catharina M.; Andresen, Thomas Lars; Hædersdal, Merete

doi:10.1002/lsm.23013

Cited by 26 publications

(23 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…good correlation with previous in vitro results (Olesen et al, 2019). In contrast, hydrophilic anticancer drugs cisplatin and 5-fluorouracil have displayed markedly lower concentrations in vivo than in vitro (Wenande et al, 2018a;Wenande et al, 2017;Wenande et al, 2018b).…”

Section: Discussionsupporting

confidence: 88%

“…Ablative fractional lasers (AFLs) have been shown to enhance topical uptake of various drugs throughout the dermal layers by penetrating the skin in a grid of discrete microchannels, increasing clinical efficacy in treatment of skin malignancies (Choi et al, 2015;Haedersdal et al, 2014;Lee et al, 2011;Togsverd-Bo et al, 2015;Wenande et al, 2018b). Recently, substantially enhanced skin uptake of vismodegib has been shown in vitro and achieving concentrations well above previously reported plasma concentrations for oral vismodegib administration (Olesen et al, 2019). Similarly, enhanced delivery of topical vismodegib has been shown in vitro using microneedles, another physical method for increasing cutaneous drug uptake (Nguyen and Banga, 2015), and recently topical vismodegib uptake has been shown using specialized drug formulations (Briand et al, 2017;Calienni et al, 2019;Kandekar et al, 2019).…”

Section: Introductionmentioning

confidence: 96%

See 1 more Smart Citation

Enhanced and Sustained Cutaneous Delivery of Vismodegib by Ablative Fractional Laser and Microemulsion Formulation

Olesen

Clergeaud

Hendel

et al. 2020

Journal of Investigative Dermatology

Self Cite

View full text Add to dashboard Cite

Section: Discussionsupporting

confidence: 88%

Section: Introductionmentioning

confidence: 96%

Enhanced and Sustained Cutaneous Delivery of Vismodegib by Ablative Fractional Laser and Microemulsion Formulation

Olesen

Clergeaud

Hendel

et al. 2020

Journal of Investigative Dermatology

Self Cite

View full text Add to dashboard Cite

“…Transdermal drug delivery system (TDDS) is a system that delivers various drugs through the skin into the systemic circulation at a predetermined and controlled rate . As a new method of drug delivery, TDDS has its own unique advantages of avoiding first‐pass drug metabolism by liver and gastrointestinal hydrolytic enzymes and maintaining constant blood concentration . Physiologically, only a small amount of lipophilic molecules with molecular weight below 1 kDa can passively penetrate the skin.…”

Section: Introductionmentioning

confidence: 99%

Transdermal delivery of water‐soluble fluorescent antibody mediated by fractional Er:YAG laser for the diagnosis of lupus erythematosus in mice

et al. 2018

View full text Add to dashboard Cite

Objectives Although transdermal drug delivery system (TDDS) has been successfully used for delivering small molecules, its application in the delivery of diagnostic antibodies has been limited due to their large size. In this study, we aim to obtain a broad insight in the dynamics of TRITC‐conjugated Goat Anti‐Mouse IgG (T‐IgG) uptake in fractional Er:YAG laser pretreated skin and provide a new technical option for detecting lupus erythematosus (LE) in mice. Methods The skins of SD and MRL/lpr mice were treated by fractional Er:YAG laser followed by external application of T‐IgG. The classic Franz diffusion method was used to observe the effects of different fractional fluences, densities and antibody concentrations on transdermal delivery of T‐IgG at different time points (2, 4, 6, 8, 20, and 24 hours). Frozen tissue sections and confocal microscopy were used to observe the distribution of T‐IgG on the sagittal and coronal planes of murine skin. Results Increased laser fluence (12.5 J/cm2 to 37.5 J/cm2) within 24 hours resulted in the obvious increase in transdermal amounts of T‐IgG during the early stage (before 8 hours). However, increasing laser density (100 pores/cm2 to 200 pores/cm2) produced a significant increase in T‐IgG permeation during the late stage (20 and 24 hours). Unlike fluence and density, increase in T‐IgG loading concentration (0.5 to 2 μg/μl) led to continuous increase in the whole process of transdermal delivery. T‐IgG appeared in the micro‐pores of SD mice skin within 4 hours after treatment in vivo. After 24 hours, it was observed in the skin. In MRL/lpr mice, positive lupus band testing (LBT) could be found on the skin lesion after laser and T‐IgG external application. Conclusions Fractional Er:YAG laser can help antibodies (150 kDa) to implement effective and controllable transdermal delivery. LBT can be achieved in MRL/lpr mice using TDDS in vivo, which may contribute to the minimally invasive diagnosis of LE. Lasers Surg. Med. 51:268–277, 2019. © 2018 Wiley Periodicals, Inc.

show abstract

“…AFXL creates an array of microscopic ablation channels through which topical drugs can be delivered, while damage to the skin is minimized. Pretreatment with AFXL has proven to increase uptake of numerous topical substances, including photosensitizers, anesthetics, corticosteroids, methotrexate, and vismodegib . In clinical trials, AFXL assisted photodynamic therapy has led to more effective treatments for actinic keratoses .…”

Section: Introductionmentioning

confidence: 99%

Drug penetration enhancement techniques in ablative fractional laser assisted cutaneous delivery of indocyanine green

et al. 2019

View full text Add to dashboard Cite

Background and Objectives Topical drug delivery can be increased by pretreatment of the skin with ablative fractional laser (AFXL). Several physical penetration enhancement techniques have been investigated to further improve AFXL‐assisted drug delivery. This study investigated the influence of three of these techniques, namely massage, acoustic pressure wave treatment, and pressure vacuum alterations (PVP) on the distribution of the fluorescent drug indocyanine green (ICG) at different depths in the skin after topical application on AFXL pretreated skin. Materials and Methods In ex vivo human skin, test regions were pretreated with AFXL (10,600 nm, channel depth 300 μm, channel width 120 μm, density 15%). Subsequently, ICG was applied, followed by massage, acoustic pressure wave treatment or PVP. ICG fluorescence intensity (FI) was assessed after 1, 3, and 24 hours at several depths using fluorescence photography. Results FI was higher when using enhancement techniques compared to control (AFXL‐only) up to 3 hours application time (P < 0.05). After 3 hours, mean surface FI was highest after acoustic pressure wave treatment (61.5 arbitrary units; AU), followed by massage (57.5AU) and PVP (46.9AU), respectively (for comparison: AFXL‐only 31.6AU, no pretreatment 14.9AU). Comparable or higher FI was achieved already after 1 hour with enhancement techniques compared to 3–24 hours application time without. After 24 hours, no significant differences between enhancement techniques and AFXL‐only were observed (P = 0.31). Conclusion Penetration enhancement techniques, especially acoustic pressure wave treatment and massage, result in improved drug accumulation in AFXL‐pretreated skin and reduce the application time needed. Lasers Surg. Med. © 2019 The Authors. Lasers in Surgery and Medicine Published by Wiley Periodicals, Inc.

show abstract

Topical delivery of vismodegib using ablative fractional laser and micro‐emulsion formulation in vitro

Abstract: AFL enhances topical delivery of micro-emulsion formulated vismodegib, reaching concentrations similar to or above plasma concentrations previously reported in patients receiving oral vismodegib. Lasers Surg. Med. © 2018 Wiley Periodicals, Inc.

Cited by 26 publications

References 22 publications

Enhanced and Sustained Cutaneous Delivery of Vismodegib by Ablative Fractional Laser and Microemulsion Formulation

Enhanced and Sustained Cutaneous Delivery of Vismodegib by Ablative Fractional Laser and Microemulsion Formulation

Transdermal delivery of water‐soluble fluorescent antibody mediated by fractional Er:YAG laser for the diagnosis of lupus erythematosus in mice

Drug penetration enhancement techniques in ablative fractional laser assisted cutaneous delivery of indocyanine green

Contact Info

Product

Resources

About