Topical DNA Vaccination with DNA/Lipid Based Complex

Choi, Myeong Jun; Kim, Jong Heon; Maibach, Howard I.

doi:10.2174/156720106775197484

Cited by 31 publications

(13 citation statements)

References 55 publications

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“…SLNs and NLCs have been used for topical application[10] for various drugs such as tropolide[47], imidazole antifungals[14], anticancers[48], vitamin A[49], isotretinoin[50], ketoconazole[51], DNA[52], flurbiprofen[53] and glucocorticoids[54]. The penetration of podophyllotoxin-SLN into stratum corneum along with skin surface lead to the epidermal targeting[48].…”

Section: Applicationsmentioning

confidence: 99%

Solid lipid nanoparticles: A modern formulation approach in drug delivery system

Mukherjee¹,

Ray²,

Thakur³

2009

Indian J Pharm Sci

789

390

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Solid lipid nanoparticles are at the forefront of the rapidly developing field of nanotechnology with several potential applications in drug delivery, clinical medicine and research, as well as in other varied sciences. Due to their unique size-dependent properties, lipid nanoparticles offer the possibility to develop new therapeutics. The ability to incorporate drugs into nanocarriers offers a new prototype in drug delivery that could be used for secondary and tertiary levels of drug targeting. Hence, solid lipid nanoparticles hold great promise for reaching the goal of controlled and site specific drug delivery and hence have attracted wide attention of researchers. This review presents a broad treatment of solid lipid nanoparticles discussing their advantages, limitations and their possible remedies. The different types of nanocarriers which were based on solid lipid like solid lipid nanoparticles, nanostructured lipid carriers, lipid drug conjugates are discussed with their structural differences. Different production methods which are suitable for large scale production and applications of solid lipid nanoparticles are described. Appropriate analytical techniques for characterization of solid lipid nanoparticles like photon correlation spectroscopy, scanning electron microscopy, differential scanning calorimetry are highlighted. Aspects of solid lipid nanoparticles route of administration and their biodistribution are also incorporated. If appropriately investigated, solid lipid nanoparticles may open new vistas in therapy of complex diseases.

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Section: Applicationsmentioning

confidence: 99%

Solid lipid nanoparticles: A modern formulation approach in drug delivery system

Mukherjee¹,

Ray²,

Thakur³

2009

Indian J Pharm Sci

789

390

View full text Add to dashboard Cite

show abstract

“…Many different strategies have been in focus for improved in vivo DNA delivery. These include formulations such as lipids [39], salts [40], local anesthetics [41], sugars [42], and devices such as the gene gun [43] and the Biojet [44].…”

Section: Electroporationmentioning

confidence: 99%

DNA vaccines: developing new strategies to enhance immune responses

2008

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We have focused our research on understanding the basic biology of and developing novel therapeutic and prophylactic DNA vaccines. We have among others three distinct primary areas of interest which include: 1. Enhancing in vivo delivery and transfection of DNA vaccine vectors 2. Improving DNA vaccine construct immunogenicity 3. Using molecular adjuvants to modulate and skew immune responses. Key to the immunogenicity of DNA vaccines is the presentation of expressed antigen to antigen-presenting cells. To improve expression and presentation of antigen, we have investigated various immunization methods with current focus on a combination of intramuscular injection and electroporation. To improve our vaccine constructs, we also employed methods such as RNA/codon optimization and antigen consensus to enhance expression and cellular/humoral cross-reactivity, respectively. Our lab also researches the potential of various molecular adjuvants to skew Th1/Th2 responses, enhance cellular/humoral responses, and improve protection in various animal models. Through improving our understanding of basic immunology as it is related to DNA vaccine technology, our goal is to develop the technology to the point of utility for human and animal health.

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“…This goal can be reached by the use of adjuvants that are able to improve the immunogenicity. Adjuvants have been used for about 70 years even before the mechanism of their action had been understood (Choi et al 2006). Nowadays, according to several studies, adjuvants can be classified by their mode of action that includes immunomodulation, presentation, induction of cytotoxic T lymphocytes, targeting and depot formation (Cox and Coulter 1997;Horzinek et al 1997;Bowersock and Martin 1999;Khan et al 2007;Gregorio et al 2009).…”

Section: Discussionmentioning

confidence: 99%