Evaluation of different adjuvants formulations for bluetongue vaccine

Macedo, Ludmila Branco; Lobato, Zélia Inês Portela; Fialho, Sílvia Ligório; Viott, Aline de Marco; Guedes, Roberto Maurício Carvalho; Silva-Cunha, Armando

doi:10.1590/s1516-89132013005000002

Cited by 5 publications

(1 citation statement)

References 31 publications

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“… 39 Adjuvants have been used for more than 70 years even when the mode of their action had not been understood. 40 The use of adjuvants in inactivated FMD vaccine formulation plays a vital role in improving the development of rapid and long-lasting protective immunity. Thus, the findings of the present study are imperative in contributing valuable information in the understanding of the effective formulations of FMD vaccines and selection of adjuvants used in the eradication of the disease.…”

Section: Discussionmentioning

confidence: 99%

Combined Adjuvant Formulations Enhanced an Immune Response of Trivalent Foot and Mouth Disease Vaccine in Cattle

Ayele¹,

Getachew²,

Bari³

et al. 2023

VMRR

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Introduction Foot-and-mouth disease is globally one of the most economically important viral diseases of cloven-hoofed animals that can be controlled by different strategies, where vaccination plays an important role. Selection of adjuvant added to vaccine preparation is crucial in ensuring the protective effect of the vaccine. Aluminum hydroxide gel mixed with saponin (AS) is widely used adjuvant, with its suboptimal immune response in FMD vaccine. The present study was undertaken to evaluate different ingredients of adjuvants for inactivated trivalent (A, O and SAT 2) FMD vaccine and to demonstrate the effect of booster dose in cattle. Methods Cattle were grouped into five; four experimental and one control, with six animals in each group and immunized with trivalent vaccine with various formulations of adjuvants. Immune response was measured using Solid Phase Competitive Enzyme Linked Immune Sorbent Assay (SPCE). Results The antibody level in cattle immunised with a vaccine formulation containing a mixture of aluminum hydroxide gel and saponin (AS) were significantly lower than AS boosted group for the three serotypes (p<0.05, t -test), which directs the need for booster dose. Whereas the antibody response in the AS + oil group was higher followed by oil alone. The AS preparation with a booster dose has shown better immune response compared to the group without. Conclusion The findings of this study could suggest that oil based and AS with oil could replace the conventional aluminum hydroxide gel and saponin adjuvants in FMD vaccine preparations. Challenge test was not successful indicating the need for further research on the virus infectivity.

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Section: Discussionmentioning

confidence: 99%

Combined Adjuvant Formulations Enhanced an Immune Response of Trivalent Foot and Mouth Disease Vaccine in Cattle

Ayele¹,

Getachew²,

Bari³

et al. 2023

VMRR

View full text Add to dashboard Cite

show abstract

Development of novel cationic microemulsion as parenteral adjuvant for influenza vaccine

Lamaisakul

Tantituvanont

Lipipun

et al. 2020

Asian Journal of Pharmaceutical Sciences

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Evaluation of different adjuvant formulations of trivalent Foot and Mouth Disease vaccine in cattle

Ayele¹,

Getachew²,

Bari

et al. 2022

Preprint

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Background Foot-and-mouth disease is globally one of the most economically important viral diseases of cloven-hoofed animals that can be controlled by different strategies, where vaccination plays an important role. Selection of the adjuvants,that added to the vaccine is crucial in ensuring the protective effect of the vaccine. Aluminum hydroxide gel and saponin (AS) is widely used adjuvant, with its poor immune response in FMD vaccine. The present study was undertaken to evaluate different ingredients of adjuvants for inactivated trivalent (A, O and SAT 2) FMD vaccine and to demonstrate the effect of booster dose in cattle. Results Cattle were grouped into five; four experimental and one control, with six animals in each group and immunized with trivalent vaccine with various formulations of adjuvants. Immune response was measured using Solid Phase Competitive Enzyme Linked Immune Sorbent Assay (SPCE). The antibody level in cattle immunised with a vaccine formulation containing a mixture of aluminum hydroxide gel and saponin (AS) were significantly lower than AS boosted group for the three serotypes (p < 0.05, t. test), which directs the need for booster dose. Whereas the antibody response in the AS + oil group was higher followed by oil alone, AS boosted and AS at 95% CI. Conclusion The findings of this study could suggest that oil based and AS with oil could replace the conventional aluminum hydroxide gel and saponin adjuvants in FMD vaccine preparations. On different note, challenge test was not successful in this study indicating the need for further research on the virus infectivity.

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Evaluation of different adjuvants formulations for bluetongue vaccine

Abstract: This study investigated the adjuvant potential of W/O/W multiple emulsions and microemulsions, comparing them with traditional aluminum hydroxide and oil-in-water emulsion adjuvants against bluetongue vaccine (BTV). Local inflammatory reactions were assessed in rabbits by

Cited by 5 publications

References 31 publications

Combined Adjuvant Formulations Enhanced an Immune Response of Trivalent Foot and Mouth Disease Vaccine in Cattle

Combined Adjuvant Formulations Enhanced an Immune Response of Trivalent Foot and Mouth Disease Vaccine in Cattle

Development of novel cationic microemulsion as parenteral adjuvant for influenza vaccine

Evaluation of different adjuvant formulations of trivalent Foot and Mouth Disease vaccine in cattle

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