Topical grape (Vitis vinifera) seed extract promotes repair of full thickness wound in rabbit

Abstract

In recent years, oxidative stress and free radicals have been implicated in impaired wound healing. Grape (Vitis vinifera) seed extract (GSE) possesses anti-inflammatory and antioxidant properties. The present study was undertaken to assess the potential activity of grape seed hydroalcoholic extract in wound healing in rabbits. Rabbits of either sex were subjected to a 20 × 20 mm square excision made over the skin of the back. The animals were randomly divided into seven experimental groups, as negative and positive control, eucerin and treatments. Negative control group did not receive any treatment. Positive control and eucerin groups received phenytoin cream (1%) and topical eucerin, respectively, twice a day from the beginning of experiments to complete wound closure. Treatment groups were treated topically by cream of GSE (2, 5, 10 and 70% w/w) in eucerin base, twice daily. For evaluation of the percentage of wound healing, area of the wound was measured daily. Histological studies were performed on the 7th and 15th days of treatments. After complete healing, hydroxyproline content and tensile strength measurement of tissue samples were done. Results showed that there were statistically significant differences between GSE treatments groups and eucerin animals (P < 0·05) in most of the days. Rabbits treated with 2% GSE had best results (completed healing in 13 days, higher hydroxyproline content and higher tissue resistance). We concluded that the extract of 2% GSE administered topically has a good potential to promote wound healing in wound model of rabbits.

Skin Wound Healing and Phytomedicine: A Review

“…Grape (Vitis vinifera) seed extract exhibits anti-inflammatory and antioxidant features [19]. It has been explained that topical application of grape seed proanthocyanidin extract promotes wound restoration.…”

Review of the Pharmacological Effects ofVitis vinifera(Grape) and its Bioactive Constituents: An Update

“…Furthermore, topical grape seed extract 2% w/w in a eucerin base had significant wound healing effects in rabbits and this effect was greater than that of topical phenytoin (Hemmati et al ., ).…”

The Effects of Grape Seed Extract Ointment on Approximation of Cesarean Section Wound

“…In line with our findings, their findings confirmed the positive effects of grape seed extract on wound healing. In another study, Hemmati et al (2011) also found that 2% grape seed extract accelerated the process of wound contraction and closure and reduced wound healing time from 20 to 13 days (20). Nayak et al (2010) also assessed the effects of grape skin powder on full-thickness skin lesions in mice and found that complete wound contraction and healing occurred thirteen days after the beginning of their treatment (21).…”