Topical mitomycin C effect on swine vocal folds healing

Camargo, Paulo Antonio Monteiro; Campos, Antônio Carlos Ligocki; Matias, Jorge Eduardo Fouto; Rispoli, Daniel Zeni; Przysiezny, Paulo Eduardo; Fonseca, Vinícius Ribas

doi:10.1016/s1808-8694(15)31015-6

Cited by 8 publications

(12 citation statements)

References 11 publications

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“…This result agrees with the other studies findings 11 , showing lower collagen's deposition when using mitomycin.…”

Section: Discussionsupporting

confidence: 94%

“…Camargo et al 11 , when studying a pigs' group with laser exeresis and later application of mitomycin, had a mean area of 2648.03 µm 2 concerning to the vocal fold's total collagen in the control group, while the experimental group's total collagen was 2200.30 µm 2 (P = 0.0043), demonstrating a significant difference when using mitomycin.…”

Section: Discussionmentioning

confidence: 98%

“…CO2 laser utilization, associated with other topical medications, inhibits the proliferation of cells involved in laryngeal vocal cord cicatrisation, decreasing collagen formation and contributing to postoperative vocal quality 11 .…”

Section: Groupsmentioning

confidence: 99%

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Comparative study in swines' vocal cords healing after excision of fragment with CO2 laser with mitomycin and 5-fluorouracil postoperative topical application

et al. 2009

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PURPOSE: To evaluate the deposition of collagen fibers at pig's vocal folds after topical use of mitomycin or 5-fluorouracil, when partial exeresis of mucosa layer had been promoted by CO2 laser. METHODS: There were used 18 Larger white pigs which were anesthetized and submitted to mucosa fragment's exeresis, bilaterally, at its free border. The animals were divided into 3 groups, each one with 6 animals: control group, without topical drug application; mitomycin group; and 5-fluorouracil group. After 30 days, the animals were subjected to euthanasia, and samples of the vocal folds were collected and stained by picrosirius red technique with polarization for quantification of total collagen deposition. RESULTS: In control group, the mean rate of right vocal fold's collagen deposition at submucosa consisted in a 3428.66 micrometers area. There was found an area whose size had, in average, 2196.36 micrometers, in mitomycin group, and 2269.19 micrometers, in 5-fluorouracil group. CONCLUSION: Mitomycin and 5-fluorouracil had promoted beneficial change in vocal fold's cicatrization with less collagen deposition, but there was no significant statistically difference when they were compared between themselves.

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“…This result agrees with the other studies findings 11 , showing lower collagen's deposition when using mitomycin.…”

Section: Discussionsupporting

confidence: 94%

Section: Discussionmentioning

confidence: 98%

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Comparative study in swines' vocal cords healing after excision of fragment with CO2 laser with mitomycin and 5-fluorouracil postoperative topical application

et al. 2009

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“…For modulation of wound‐healing processes and for attenuating scarring, one of the possible solutions described in the scientific literature is the application of antifibrotic drugs on primary lesions. For this purpose, various antifibrotic pharmaceutical agents such as corticosteroids, 5‐fluorouracil, carnitine, and mitomycin‐C (MMC) have already been employed either alone in short‐term local surface applications or as at our clinic in combination with preceding laser excision of cicatricle tissue in laryngotracheal stenosis treatment. Although MMC is a traditional chemotherapeutic drug, isolated from Streptomyces sp ., it has also been regarded as a compound with high potential to prevent tissue fibrosis.…”

Section: Introductionmentioning

confidence: 99%

Antifibrotic effect of mitomycin‐C on human vocal cord fibroblasts

et al. 2019

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Objective: Acquired laryngotracheal stenosis is a potentially life-threatening situation and a very difficult and challenging problem in laryngology. Therefore, new trends and innovative approaches based on antifibrotic drugs and minimally invasive regimens are being developed to attenuate laryngotracheal fibrosis and scarring. The purpose of this study was to examine the efficacy of mitomycin-C (MMC) to reverse the transforming growth factor (TGF)-β-induced differentiation of MRC-5 fibroblast and human primary vocal cord fibroblasts to reveal the possible applicability of MMC to laryngotracheal fibrotic conditions.Methods: Human primary fibroblast cells were isolated from vocal cord specimens of patients undergoing total laryngectomy. The established primary vocal cord fibroblast cell cultures as well as the MRC-5 human fibroblast cells were treated with 5 ng/mL TGF-β alone and then with 0.5 μg/mL MMC for 24 hours. Differentiation of fibroblasts was characterized by α-smooth muscle actin (α-SMA) immunhistochemistry, Western blot analysis, and real-time polymerase chain reaction. Cell motility was assessed by wound-healing assay.Results: Elevated α-SMA mRNA and protein expression as well as increased cell motility were observed upon TGF-β exposures. However, after MMC treatments the TGF-β-induced fibroblasts exhibited a significant decrease in α-SMA expression and wound-healing activity. Therefore, TGF-β-stimulated fibroblast-myofibroblast transformation was reversed at least in part by MMC treatment. Histopathological examinations of tissue specimens of a laryngotracheal stenosis patient supported these findings.Conclusion: Antifibrotic effects of MMC were demonstrated on the human MRC-5 cell line and on primary vocal cord fibroblast cultures. These results verify that MMC can be used with success to reverse upper airway stenosis by reverting the myofibroblast phenotype.

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“…Authors suggested that mitomycin C may delay but does not necessarily prevent the recurrence of stenosis. Animal studies including canines, [20][21][22][23][24] rabbits, 25 and swine 26,27 also showed the improvement of airway wound healing after mitomycin C application. Specific to the management of vocal fold scarring, topical application of mitomycin C has been shown to lead to (1) reduced anterior glottic web formation, (2) decreased total collagen deposition, (3) improved cricoarytenoid joint mobility, and (4) decreased granulation tissue development.…”

Section: Introductionmentioning

confidence: 99%

Dose‐dependent effect of mitomycin C on human vocal fold fibroblasts

Chen

Dikkers

et al. 2013

Head & Neck

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Background The purpose of this study was to evaluate in vitro cytotoxicity and antifibrotic effects of mitomycin C on normal and scarred human vocal fold fibroblasts. Methods Fibroblasts were subjected to mitomycin C treatment at 0.2, 0.5, or 1 mg/mL, or serum control. Cytotoxicity, immunocytochemistry, and Western blot for collagen I/III were performed at days 0, 1, 3, and 5. Results Significant decreases in live cells were measured for mitomycin C-treated cells on days 3 and 5 for all doses. Extracellular staining of collagen I/III was observed in mitomycin C-treated cells across all doses and times. Extracellular staining suggests apoptosis with necrosis, compromising the integrity of cell membranes and release of cytosolic proteins into the extracellular environment. Western blot indicates inhibition of collagen at all doses except 0.2 mg/mL at day 1. Conclusion A total of 0.2 mg/mL mitomycin C may provide initial and transient stimulation of collagen for necessary repair to damaged tissue without the long-term risk of fibrosis.

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Topical mitomycin C effect on swine vocal folds healing

Abstract: topical application of MTC after the exeresis of a mucous fragment of vocal fold with CO2 laser in swine significantly decreased total collagen deposition.

Cited by 8 publications

References 11 publications

Comparative study in swines' vocal cords healing after excision of fragment with CO2 laser with mitomycin and 5-fluorouracil postoperative topical application

Comparative study in swines' vocal cords healing after excision of fragment with CO2 laser with mitomycin and 5-fluorouracil postoperative topical application

Antifibrotic effect of mitomycin‐C on human vocal cord fibroblasts

Dose‐dependent effect of mitomycin C on human vocal fold fibroblasts

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